Publicações - ciências biológicas
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GEHRKE, ALINE GONÇALVES;
WIELEWSKI, LEONARDO PELLIZZARI;
WIGGERS, VINICYUS RODOLFO;
BOTTON, VANDERLEIA;
MITCHELL, DAVID ALEXANDER;
KRIEGER, NADIA
Palavra-chave:
lipase;
bio-óleo;
esterificação
Áreas do conhecimento:
Ciências Biológicas; Biotecnologia; Biotecnologia Industrial
resumo ...
Fast pyrolysis of vegetable oils and residues generates bio-oil (BO), a renewable hydrocarbon source with high acidity that limits its direct use in refineries. In this study, BOs were produced from refined soybean oil (RSO) and waste cooking oil (WCO) at 525 °C in a continuous bench-scale pyrolysis at 525 °C, with a 390 ± 8 g h−1 feed rate, under steady-state conditions. The resulting bio-oils exhibited high acidity (acid index of 145 and 127 mg KOH g−1, respectively) and elevated olefinic and oxygen contents, making them corrosive and unsuitable for co-refining with petroleum. To reduce acidity, ethyl esterification was performed using lipase B from Candida antarctica (CALB), using a Box–Behnken 33 factorial design. Variables included temperature (40–60 °C), bio-oil:ethanol mass ratio (1:1–1:5), and catalyst concentration (3–10% w/w). The acid index was reduced by up to 76%, with optimal conditions (62 °C, 1:1 mass ratio, 11% CALB) yielding a final value of 28 mg KOH g−1. Similar reductions were obtained for waste cooking oil bio-oil, confirming robustness across feedstocks. CALB retained over 70% activity after three cycles, demonstrating stability. This enzymatic esterification process shows strong potential for lowering bio-oil acidity, enabling integration into petroleum refineries, diversifying feedstocks, and advancing renewable fuel production.
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RIBEIRO, L. S.;
ZEQUI, J. A. C.;
PINHEIRO, V. C. S.;
SOARES-DA-SILVA, J.;
PALACIO-CORTÉS, A. M.;
Mário Antonio NavarroSilva;
SOUZA, I. M. S.;
SILVA, S. A. S.;
SANTOS, T. P. C.;
SILVA, R. S.;
LIMA, A. S.;
LEONARDO, F. S.
Palavra-chave:
Diptera;
Culicidae;
Aedes aegypti;
Arbovírus
Áreas do conhecimento:
Ciências Biológicas; Zoologia; Taxonomia dos Grupos Recentes;
Ciências Biológicas; Zoologia; Zoologia Aplicada; Conservação das Espécies Animais
resumo ...
Abstract Aedes aegypti is the primary vector of dengue virus (DENV), Zika virus (ZIKV), and...
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DE OLIVEIRA, FERNANDO GARRIDO;
PALMEIRO, ALESSANDRA SVONKA;
BRITO, PATRICIA ELENA MANUITT;
MOTTA, WELTON GYDEONNY;
Cestari, M.M.;
DE ASSIS, HELENA CRISTINA DA SILVA;
DOS SANTOS, LILIAN DENA
Palavra-chave:
ensaio cometa;
cascudo;
Genotoxicidade
Áreas do conhecimento:
Ciências Biológicas; Genética; Genotoxicidade em Peixes;
Ciências Biológicas; Farmacologia; toxicidade em peixes
resumo ...
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Danilo A. Zavatin;
Renato Ramos;
Jefferson Prado;
Arthur Sousa Soares;
Marcelo Monge;
Raymond M. Harley
Palavra-chave:
campos rupestres;
endemism;
Labiatae;
Nepetoideae;
Ocimeae
Áreas do conhecimento:
Ciências Biológicas; Botânica; Taxonomia Vegetal; Taxonomia de Fanerógamos
resumo ...
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MOREIRA SILVA, ENZO ZINI;
RODRIGUES, ANA CAROLINA;
KOHLER, ANA FLÁVIA;
CRUZ, JULIANA VARELLA;
PRADO, KARIN BRAUN;
Daniela Fiori Gradia;
DE OLIVEIRA, DANIELLE PALMA;
PESTANA, CYNTHIA BOMFIM;
LEME, DANIELA MORAIS
Palavra-chave:
Organophosphorus flame retardants;
Reconstructed human epidermis;
In silico prediction;
Immortalized human keratinocyte;
Cytokine release;
DNA methylation
Áreas do conhecimento:
Ciências Biológicas; Biotecnologia; Biotecnologia em Saúde Humana e Animal; Metodologias Alternativas ao Uso de Animais
resumo ...
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PELLIZZARI WIELEWSKI, LEONARDO;
LUJAN FERREIRA, MARIA;
ALNOCH, ROBSON CARLOS;
MITCHELL, DAVID ALEXANDER;
KRIEGER, NADIA
Palavra-chave:
lipase;
bioimprinting
Áreas do conhecimento:
Ciências Biológicas; Bioquímica; Enzimologia; Biotecnologia
resumo ...
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KAVA, VANESSA;
ORDONEZ, JUAN;
OLIVEIRA, ANNE;
MARIANO, ANDRÉ B.;
CONOR, GABRIELA;
Jóse Viriato Coelho Vargas;
MELENEK, NICHOLAS;
RAMPI, MURILO
Palavra-chave:
agricultural practices;
plant growth promoters;
mathematical model;
sustainability;
cogeneration;
exergy analysis
Áreas do conhecimento:
Engenharias; Engenharia Mecânica; Engenharia Térmica; Aproveitamento da Energia;
Ciências Biológicas; Biotecnologia; Biotecnologia Industrial
resumo ...
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DE OLIVEIRA, KETELEN MICHELE GUILHERME;
MAGALHÃES, WASHINGTON LUIZ ESTEVES;
LEME, DANIELA MORAIS;
Rayssa Barbary Pedrosa Moura;
Stephânia Fleury Taveira;
Helena Lourenço Zielonka;
Darwin de Oliveira Brum;
Enzo Zini Moreira Silva;
Edvaldo da Silva Trindade;
Cestari, M.M.;
Pedro Henrique Gonzalez de Cadermatori
Palavra-chave:
cultivo celular;
citotoxicidade
Áreas do conhecimento:
Ciências Biológicas; Biologia Geral; citotoxicidade;
Ciências Biológicas; Genética; genotoxicidade
resumo ...
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PALACIO-CORTÉS, ANGELA MARIA;
VALENCIA-MARÍN, BRYAN STEVEN;
Mário Antonio NavarroSilva
Palavra-chave:
Diptera;
Culicidae;
Aedes albopictus;
ND5
Áreas do conhecimento:
Ciências Biológicas; Zoologia; Taxonomia dos Grupos Recentes;
Ciências Biológicas; Genética; Genética Animal
resumo ...
Aedes (Stegomyia) albopictus, commonly known as the Asian Tiger mosquito, is native to tropical and subtropical regions of South and East Asia and has rapidly spread globally. Due to its role as a vector of several medically important arboviruses, understanding its genetic diversity and dispersal patterns is crucial for effective disease control. This study aimed to evaluate the genetic structure insights of Ae. albopictus populations in southeastern Brazil. Mosquito samples from five populations in southeastern Brazil were analyzed using two mitochondrial genes: cytochrome c oxidase subunit 1 (COI) and NADH dehydrogenase subunit 5 (ND5). Phylogenetic analysis and population genetic metrics were employed to assess patterns of genetic diversity and population structure. Moderate haplotype diversity was observed, with five COI haplotypes (Hd = 0.43) and eleven ND5 haplotypes (Hd = 0.52), along with low nucleotide diversity. Neutrality tests for ND5 yielded significantly negative values (Fs = -2.435*). Phylogenetic trees identified two major clades, with ND5 haplotypes from Paranaguá and Guaraqueçaba clustering together. A positive correlation between genetic and geographic distance (COI r = 0.78; ND5 r = 0.69) proposing isolation by distance. The close genetic relationships and limited variation among Brazilian Ae. albopictus populations indicate ongoing gene flow and a shared ancestry. The association of Paraná haplotypes with Asian lineages suggests a genetic link to the region of origin of species, although the timeline of introduction remains uncertain. These results provide important molecular insights to support vector surveillance and control efforts in southern Brazil.
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DA SILVA, ANA CAROLINA FELIPE;
PEREIRA, LETÍCIA DA SILVA;
BRANT, RODRIGO SOARES CALDEIRA;
VICENTINI, MAIARA;
Helena Cristina da Silva de Assis
Palavra-chave:
Proteômica
resumo ...
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