Publicações - ciências biológicas
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LEAO, ANIELE C. RIBAS;
BAURA, VALTER;
Helisson Faoro;
SFEIR, MICHELLE ZIBETTI TADRA;
BALSANELLI, EDUARDO;
MORENO, LEANDRO F.;
NAJAFZADEH, M. JAVAD;
DE HOOG, SYBREN;
Emanuel Maltempi de Souza;
WEISS, VINICIUS ALMIR;
VICENTE, VANIA APARECIDA;
COSTA, FLAVIA;
BOMBASSARO, AMANDA;
RAITTZ, ROBERTO TADEU;
STEFFENS, M;
PEDROSA, FÁBIO OLIVEIRA;
GOMES, RENATA R.
Palavra-chave:
Fonsecaea multimorphosa;
draft genome sequence
Áreas do conhecimento:
Ciências Biológicas; Microbiologia; Biologia e Fisiologia dos Microorganismos; Virologia;
Ciências Biológicas; Bioquímica; Bioinformática
resumo ...
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Camila Candido;
Ana Teresa Lombardi
Palavra-chave:
vinhaça;
Toxicidade;
fisiologia algal
Áreas do conhecimento:
Ciências Biológicas; ECOTOXICOLOGIA
resumo ...
Vinasse is a residue of the sugarcane industry. It can be biodigested or not, in this case referred as conventional. The conventional or biodigested vinasses have high content of organic matter and mineral elements, leading to their common use as soil fertilizer for the sugarcane crop. However, vinasses are toxic residues and they can salinize the soil if used too much. On the other hand, the production of photosynthetic microalgae is costly and using a residue to support its growth may contribute to cost reduction. However, because of the vinasse dark color and toxicity, high dilution is necessary to accomplish microalgal growth. Here we present results on the growth and biomass yield of Chlorella vulgaris in conventional and in biodigested vinasses that have been treated by filtration or centrifugation before their use as microalgae culture medium. A concentration range of 10 to 100 % was tested and microalgal growth occurred in vinasse concentration as high as 80 %, with no nutrient addition. We evaluated pH, electrical conductivity, absorbance at 570 nm, and cell density every 24 h in a 6-day incubation experiment. Specific growth rates were calculated and the results showed that in 60 % filtered conventional and 80 % biodigested vinasses, C. vulgaris performed as well as the controls in nutrient rich synthetic culture media, with growth rates of up to 1.2 day−1. Thus, we propose the use of treated vinasse as culture medium for lowering the costs of microalgae production, with the advantage of increasing the residue value.
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Maria Rosa Dmengeon P de Souza;
Lucelia Donatti;
Tatiana Herrerias;
Tânia Zaleski;
Mariana Forgati;
Priscila K Kandalski;
Cíntia Machado;
Dilza T Silva;
Cláudio A Piechnik;
Mauricio Osvaldo Moura
Palavra-chave:
adaptação;
stress térmico
Áreas do conhecimento:
Ciências Biológicas; Ecologia; Ecologia Aplicada
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VALDAMERI, GLAUCIO;
KRIEGER, NADIA;
MOURE, VIVIAN ROTUNO;
KOKOT, THIAGO BORBA;
KUKOLJ, CAROLINE;
KRIEGER, NADIA;
Rose Adele Monteiro;
PEDROSA, FABIO DE OLIVEIRA;
Emanuel Maltempi de Souza
Áreas do conhecimento:
Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia;
Ciências Biológicas; Bioquímica; Bioquímica dos Microorganismos
resumo ...
Herbaspirillum rubrisubalbicans decreases growth of rice. Inoculation of rice with H. rubrisubalbicans
increased the ACCO mRNA levels and ethylene production. The H. rubrisubalbicans rice
interactions were further characterized by proteomic approach.
Herbaspirillum rubrisubalbicans is a well-known growth-promoting rhizobacteria that can also act as a mild phyto-pathogen. During colonisation of rice, RT-qPCR analyses showed that H. rubrisubalbicans up-regulates the methionine recycling pathway as well as phyto-siderophore synthesis genes. mRNA levels of ACC oxidase and ethylene levels also increased in rice roots but inoculation with H. rubrisubalbicans impaired growth of the rice plant. A proteomic approach was used to identify proteins specifically modulated by H. rubrisubalbicans in rice and amongst the differentially expressed proteins a V-ATPase and a 14-3-3 protein were down-regulated. Several proteins of H. rubrisubalbicans were identified, including the type VI secretion system effector Hcp1, suggesting that protein secretion play a role colonisation in rice. Finally, the alkyl hydroperoxide reductase, a primary scavenger of endogenous hydrogen peroxide was also identified. Monitoring the levels of reactive oxygen species in the epiphytic bacteria by flow cytometry revealed that H. rubrisubalbicans is subjected to oxidative stress, suggesting that the alkyl hydroperoxide reductase is an important regulator of redox homeostasis in plant-bacteria interactions.
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Daniele Chaves-Moreira;
Andrea Senff Ribeiro;
Wille, Ana Carolina Martins;
Luiza Helena Gremski;
OLGA CHAIM;
Silvio Sanches Veiga
The Journal of Venomous Animals and Toxins Including Tropical Diseases (Online),
v. 23,
n. 6,
p. 1-10,
2017
DOI
Palavra-chave:
Aranha marrom;
Veneno;
Toxinas;
Avaliação biológica
Áreas do conhecimento:
Ciências Biológicas; Morfologia; Citologia e Biologia Celular;
Ciências Biológicas; Bioquímica; Biologia Molecular
resumo ...
Brown spiders are venomous arthropods that use their venom for predation and defense. In humans, bites of these animals provoke injuries including dermonecrosis with gravitational spread of lesions, hematological abnormalities and impaired renal function. The signs and symptoms observed following a brown spider bite are called loxoscelism. Brown spider venom is a complex mixture of toxins enriched in low molecular mass proteins (4–40 kDa). Characterization of the venom confirmed the presence of three highly expressed protein classes: phospholipases D, metalloproteases (astacins) and insecticidal peptides (knottins). Recently, toxins with low levels of expression have also been found in Loxosceles venom, such as serine proteases, protease inhibitors (serpins), hyaluronidases, allergen-like toxins and histamine-releasing factors. The toxin belonging to the phospholipase-D family (also known as the dermonecrotic toxin) is the most studied class of brown spider toxins. This class of toxins single-handedly can induce inflammatory response, dermonecrosis, hemolysis, thrombocytopenia and renal failure. The functional role of the hyaluronidase toxin as a spreading factor in loxoscelism has also been demonstrated. However, the biological characterization of other toxins remains unclear and the mechanism by which Loxosceles toxins exert their noxious effects is yet to be fully elucidated. The aim of this review is to provide an insight into brown spider venom toxins and toxicology, including a description of historical data already available in the literature. In this review article, the identification processes of novel Loxosceles toxins by molecular biology and proteomic approaches, their biological characterization and structural description based on x-ray crystallography and putative biotechnological uses are described along with the future perspectives in this field.
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DUTRA, FABRÍCIO MARTINS;
RONNAU, MILTON;
SPONCHIADO, DIRCELEI;
FORNECK, SANDRA CARLA;
Carolina Arruda de Oliveira Freire;
BALLESTER, EDUARDO LUIS CUPERTINO
Palavra-chave:
Macrobrachium;
Ammonia;
Nitrito;
Histologia
Áreas do conhecimento:
Ciências Biológicas; Fisiologia Comparada; Fisiologia Comparativa da Osmorregulação
resumo ...
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DORIA, HALINA BINDE;
VOIGT, CARMEN LÚCIA;
SANDRINI-NETO, LEONARDO;
CAMPOS, SANDRO XAVIER;
DE OLIVEIRA-RIBEIRO, CIRO ALBERTO;
M. A. F. Randi
Palavra-chave:
Bioaccumulation;
Biomarkers;
Chronic toxicity;
Geophagus brasiliensis;
reservoir;
toxic metals
Áreas do conhecimento:
Ciências Biológicas; Ecologia; Ecologia Aplicada
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The Alagados Reservoir located in the Brazilian city of Ponta Grossa is used to supply water for human consumption but is impacted by toxic metals. The current study combined chemical, biochemical, and multivariate analyses to determine the bioavailability of toxic metals at three sites (AL-A, AL-B, and AL-C) within the Alagados Reservoir. Metal bioaccumulation was analyzed in the liver, gills, and muscle tissue of a native fish species (Geophagus brasiliensis), and neurotoxicity, xenobiotic metabolism, and oxidative stress were evaluated using biochemical biomarkers. Additionally, histopathological studies were performed on the gills and the liver using scanning electron microscopy (SEM) and conventional light microscopy (LM), respectively. Overall, the bioaccumulation of metals, biomarkers of oxidative stress, and melanomacrophage counts indicate that the AL-C and the AL-A sites are the most and least affected by metals, respectively. The AL-B site presented the lowest acetylcholinesterase enzyme activity, a finding which was probably associated with the agricultural activities around this area of the reservoir. The biomarkers clearly revealed that toxic metals negatively affect all three sites studied herein and that human activity is the major source of pollutants. Despite the existence of different pollution levels within the Alagados Reservoir, it is still used as a human water supply.
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Oscar Alexander Aguirre-Obando;
Mário Antonio NavarroSilva
Palavra-chave:
Diptera;
Aedes albopictus;
Culicidae;
Insetos vetores
Áreas do conhecimento:
Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia;
Ciências Biológicas; Zoologia; Zoologia Aplicada; Conservação das Espécies Animais;
Ciências Biológicas; Parasitologia; Entomologia e Malacologia de Parasitos e Vetores
resumo ...
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VIDAL, LUINE ROSELE RENAUD;
Barbara Maria Cavalli;
ALMEIDA, SÉRGIO MONTEIRO DE;
RABONI, SONIA MARA;
NOGUEIRA, MERI BORDIGNON
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RIBAS, JOÃO L.C.;
SHERRY, JAMES P.;
Aleksander Roberto Zampronio;
SILVA DE ASSIS, HELENA C.;
SIMMONS, DENINA B.D.
Palavra-chave:
proteomics;
cytokine;
fish;
nitric oxide
Áreas do conhecimento:
Ciências Biológicas; Farmacologia Bioquímica e Molecular;
Ciências Biológicas; Farmacologia; Toxicologia; Toxicologia Ambiental
resumo ...
Nonsteroidal anti‐inflammatory drugs are among the most widely detected pharmaceuticals in surface water worldwide. The nonsteroidal anti‐inflammatory drug diclofenac is used to treat many types of ...
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