Publicações - ciências biológicas
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morgana de araujo evangelista;
Fernando Augusto Lavezzo Dias;
Mario Emilio Dourado Junior;
GUALDI, LUCIEN P.;
Andrea Aliverti;
Resqueti, Vanessa R.;
Fregonezi, Guilherme A. F.
Palavra-chave:
respiratory muscle;
diaphragm;
myotonic dystrophy
Áreas do conhecimento:
Ciências da Saúde; Fisioterapia Respiratória;
Ciências Biológicas; Fisiologia; Fisiologia de Órgãos e Sistemas; Fisiologia da Respiração
resumo ...
Objective To evaluate sensitivity/specificity of the maximum relaxation rate (MRR) of inspiratory muscles, amplitude of electromyographic activity of the sternocleidomastoid (SCM), scalene (SCA), parasternal (2ndIS) and rectus abdominis (RA) muscles; lung function and respiratory muscle strength in subjects with Myotonic dystrophy type 1 (DM1) compared with healthy subjects. Design and methods Quasi-experimental observational study with control group. MRR of inspiratory muscles, lung function and amplitude of the electromyographic activity of SCM, SCA, 2ndIS and RA muscles during maximum inspiratory pressure (PImax), maximum expiratory pressure (PEmax) and sniff nasal inspiratory pressure (SNIP) tests were assessed in eighteen DM1 subjects and eleven healthy. Results MRR was lower in DM1 group compared to healthy (P = 0.001) and was considered sensitive and specific to identify disease in DM1 and discard it in controls, as well as SNIP% (P = 0.0026), PImax% (P = 0.0077) and PEmax% (P = 0.0002). Contraction time of SCM and SCA was higher in DM1 compared to controls, respectively, during PImax (P = 0.023 and P = 0.017) and SNIP (P = 0.015 and P = .0004). The DM1 group showed lower PImax (P = .0006), PEmax (P = 0.0002), SNIP (P = 0.0014), and higher electromyographic activity of the SCM (P = 0.002) and SCA (P = 0.004) at rest; of 2ndIS (P = 0.003) during PEmax and of SCM (P = 0.02) and SCA (P = 0.03) during SNIP test. Conclusions MD1 subjects presented restrictive pattern, reduced respiratory muscle strength, muscular electrical activity and MRR when compared to higher compared to controls. In addition, the lower MRR found in MD1 subjects showed to be reliable to sensitivity and specificity in identifying the delayed relaxation of respiratory muscles.
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Carla Adriane Royer;
Antonio Luis Vieira Toscano de Brito;
Eric de Camargo Smidt
Palavra-chave:
biodiversity;
Flora do Paraná;
IUCN Red List;
Mata Atlântica
resumo ...
Resumo Zygostates consiste de ca. 26 espécies, das quais 15 ocorrem no Brasil. Através de...
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lucianne Leigue;
Ayrton Rodrigo Hilgert;
Adriana Fiorini;
Marise Fonseca dos Santos;
Eliane Cristina Gruszka Vendruscolo
Palavra-chave:
Staphylococcus aureus;
Ardra;
mecA gene;
MRSA
Áreas do conhecimento:
CIENCIAS_AGRARIAS; Medicina Veterinária Preventiva; Doenças Infecciosas de Animais;
Ciências Biológicas; Bioquímica; Biologia Molecular;
Ciências Biológicas; Bioquímica; Bioquímica dos Microorganismos
resumo ...
(en)
Staphylococcus aureus is one of the most common microorganisms responsible for high morbidity and mortality in humans and animals. Methicillin-resistant S. aureus are responsible for several outbreaks worldwide and therapeutic arsenal has become scarce. The present investigation verified the epidemiological profile of S. aureus strains isolated from the veterinary hospital staff, from dairy cattle workers and also from milk samples of dairy cattle presenting mastitis. Samples were characterized phenotypically by antibiogram, catalase, and coagulase tests, and also by Voges-Proskauer test. The isolated strains were characterized genotypically by specific Polymerase Chain Reaction and Amplified Ribosomal DNA Restriction Analysis (ARDRA). From the 218 isolated strains, 27 were identified as S. aureus (12%), four of them were resistant to oxacillin and two of them were classified as MRSA (Methicillin-resistant S. aureus). The prevalence of isolated strains among animal personnel care was low (2%) but all MRSA isolates were found among the clinical staff. Results of ARDRA pointed out that S. aureus strains isolated from different animal care personnel were grouped in the same cluster when HindIII and HinfII restriction enzymes were used. When ARDRA was performed with HaeIII enzyme, the formation of two clusters was observed, but the isolated strains were not correlated. The prevalence of S. aureus strains isolated was higher in clinical staff and the biochemical and molecular assays of them presented 100% of correlation.
(pt)
Staphylococcus aureus está entre os microrganismos que apresentam as maiores taxas de morbidade e mortalidade em seres humanos e animais. Linhagens de S. aureus resistentes a meticilina podem causar surtos de infecção em todo o mundo, o que contribui para a escassez de arsenal terapêutico. Este trabalho analisou o perfil epidemiológico de estirpes de S. aureus isoladas de pessoas que trabalham em contato com animais em um hospital veterinário com gado leiteiro e também em amostras de leite de vacas acometidas por mastite. As estirpes de S. aureus isoladas foram caracterizadas fenotipicamente por meio de antibiograma, testes de catalase e coagulase, e pelo teste de Voges-Proskauer. As amostras também foram caracterizadas genotipicamente pela técnica de Analise de Restricao de DNA Ribossomico Amplificado (ARDRA-PCR). Das 218 estirpes isoladas, 27 foram identificados como S. aureus (12%). Entre essas, quatro estirpes foram resistentes a oxacilina e duas classificadas como SARM (S. aureus resistente a meticilina). A ocorrência de estirpes de S.aureus isoladas entre o pessoal que trabalha em contato com os animais foi baixa (2%), mas estirpes identificadas como SARM foram encontradas na equipe clínica. As análises de ARDRA realizadas com as enzimas de restrição HindIII e HinfII demonstraram que S. aureus isolados de diferentes indivíduos que trabalhavam com animais foram agrupados no mesmo cluster. Quando a ARDRA foi realizada com HaeIII foi observada formação de dois grupos, mas os isolados não se correlacionaram. Conclusão: a ocorrência de estirpes de S. aureus isoladas foi maior na equipe clínica, apresentando também correlação de 100% nos ensaios bioquímicos e moleculares.
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lucianne Leigue;
Ayrton Rodrigo Hilgert;
Adriana Fiorini;
Marise Fonseca dos Santos;
Eliane Cristina Gruszka Vendruscolo
Palavra-chave:
Staphylococcus aureus;
Ardra;
mecA gene;
MRSA
Áreas do conhecimento:
CIENCIAS_AGRARIAS; Medicina Veterinária Preventiva; Doenças Infecciosas de Animais;
Ciências Biológicas; Bioquímica; Biologia Molecular;
Ciências Biológicas; Bioquímica; Bioquímica dos Microorganismos
resumo ...
(en)
Staphylococcus aureus is one of the most common microorganisms responsible for high morbidity and mortality in humans and animals. Methicillin-resistant S. aureus are responsible for several outbreaks worldwide and therapeutic arsenal has become scarce. The present investigation verified the epidemiological profile of S. aureus strains isolated from the veterinary hospital staff, from dairy cattle workers and also from milk samples of dairy cattle presenting mastitis. Samples were characterized phenotypically by antibiogram, catalase, and coagulase tests, and also by Voges-Proskauer test. The isolated strains were characterized genotypically by specific Polymerase Chain Reaction and Amplified Ribosomal DNA Restriction Analysis (ARDRA). From the 218 isolated strains, 27 were identified as S. aureus (12%), four of them were resistant to oxacillin and two of them were classified as MRSA (Methicillin-resistant S. aureus). The prevalence of isolated strains among animal personnel care was low (2%) but all MRSA isolates were found among the clinical staff. Results of ARDRA pointed out that S. aureus strains isolated from different animal care personnel were grouped in the same cluster when HindIII and HinfII restriction enzymes were used. When ARDRA was performed with HaeIII enzyme, the formation of two clusters was observed, but the isolated strains were not correlated. The prevalence of S. aureus strains isolated was higher in clinical staff and the biochemical and molecular assays of them presented 100% of correlation.
(pt)
Staphylococcus aureus está entre os microrganismos que apresentam as maiores taxas de morbidade e mortalidade em seres humanos e animais. Linhagens de S. aureus resistentes a meticilina podem causar surtos de infecção em todo o mundo, o que contribui para a escassez de arsenal terapêutico. Este trabalho analisou o perfil epidemiológico de estirpes de S. aureus isoladas de pessoas que trabalham em contato com animais em um hospital veterinário com gado leiteiro e também em amostras de leite de vacas acometidas por mastite. As estirpes de S. aureus isoladas foram caracterizadas fenotipicamente por meio de antibiograma, testes de catalase e coagulase, e pelo teste de Voges-Proskauer. As amostras também foram caracterizadas genotipicamente pela técnica de Analise de Restricao de DNA Ribossomico Amplificado (ARDRA-PCR). Das 218 estirpes isoladas, 27 foram identificados como S. aureus (12%). Entre essas, quatro estirpes foram resistentes a oxacilina e duas classificadas como SARM (S. aureus resistente a meticilina). A ocorrência de estirpes de S.aureus isoladas entre o pessoal que trabalha em contato com os animais foi baixa (2%), mas estirpes identificadas como SARM foram encontradas na equipe clínica. As análises de ARDRA realizadas com as enzimas de restrição HindIII e HinfII demonstraram que S. aureus isolados de diferentes indivíduos que trabalhavam com animais foram agrupados no mesmo cluster. Quando a ARDRA foi realizada com HaeIII foi observada formação de dois grupos, mas os isolados não se correlacionaram. Conclusão: a ocorrência de estirpes de S. aureus isoladas foi maior na equipe clínica, apresentando também correlação de 100% nos ensaios bioquímicos e moleculares.
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Paulo Rogério Lopes;
keila Cássia Santos Araújo;
Iara Maria Lopes;
Rafael Passos Rangel;
Paulo Yoshio Kageyama
SCIENTIFIC ELECTRONIC ARCHIVES,
v. 10,
n. 1,
p. 3-7,
2017
Áreas do conhecimento:
CIENCIAS_AGRARIAS; Entomologia;
Ciências Biológicas; Ecologia; Controle biológico
resumo ...
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Julissa Melissa Churata Salcedo;
Almeida, LM.;
Guillermo Gonzaléz;
R. D. GORDON
Palavra-chave:
Aulis;
Coccinelloidea;
Neotropical;
New records
Áreas do conhecimento:
Ciências Biológicas; Zoologia; Taxonomia dos Grupos Recentes
resumo ...
(en)
The subgenus Sidonis Mulsant, 1850 is elevated to generic status and two new species from Brazil are described and illustrated: Sidonis bira sp. nov. and Sidonis biguttata sp. nov. New geographic distribution records are provided. In addition, lectotypes of Sidonis consanguinea (Mulsant, 1850) and S. guttata (Sicard, 1912) are designated. Illustrations of diagnostic characters from five of six species of the genus, comments on the differences from similar species and a key to all recognized taxa are included.
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Rafael Baggio;
Stoiev, Sérgio;
Henry Louis Spach;
Walter Boeger
Journal of Biogeography (Print),
v. 1,
p. 1-1,
2017
Palavra-chave:
taxon pulse;
Geomorphology;
Genetic diversity
Áreas do conhecimento:
Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia
resumo ...
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Baggio, R. A.;
Sérgio Stoyev;
Henry Spach;
Walter Antonio Boeger
Palavra-chave:
Evolution;
Biogeografia;
taxon pulse
Áreas do conhecimento:
Ciências Biológicas; Evolução
resumo ...
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Luana de Oliveira Pitol;
Anelize Terezinha Jung Finkler;
Glauco Silva Dias;
Amanda Souza Machado;
Gisella Maria Zanin;
MITCHELL, DAVID ALEXANDER;
KRIEGER, NADIA
Palavra-chave:
solid state fermentation;
packed-bed bioreactor;
lipase;
fermented solid
Áreas do conhecimento:
Engenharias; Engenharia Química; Processos Industriais de Engenharia Química; Processos Bioquímicos;
Ciências Biológicas; Microbiologia; Microbiologia Aplicada; Microbiologia Industrial e de Fermentação
resumo ...
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Carolina Arruda de Oliveira Freire;
RIOS, LEONARDO DE P.;
GIARETA, ELOÍSA P.;
CASTELLANO, GIOVANNA C.
Palavra-chave:
osmorregulação;
camarão de água doce;
Macrobrachium;
Amonia;
oxigênio
Áreas do conhecimento:
Ciências Biológicas; Fisiologia Comparada; Fisiologia Comparativa da Osmorregulação
resumo ...
Palaemonid shrimps occur in the tropical and temperate regions of South America and the Indo-Pacific, in brackish/freshwater habitats, and marine coastal areas. They form a clade that recently (i.e., ~30 mya) invaded freshwater, and one included genus, Macrobrachium Bate, 1868, is especially successful in limnic habitats. Adult Macrobrachium acanthurus (Wiegmann, 1836) dwell in coastal freshwaters, have diadromous habit, and need brackish water to develop. Thus, they are widely recognized as euryhaline. Here we test how this species responds to a short-term exposure to increased salinity. We hypothesized that abrupt exposure to high salinity would result in reduced gill ventilation/perfusion and decreased oxygen consumption. Shrimps were subjected to control (0 psu) and experimental salinities (10, 20, 30 psu), for four and eight hours (n = 8 in each group). The water in the experimental containers was saturated with oxygen before the beginning of the experiment; aeration was interrupted before placing the shrimp in the experimental container. Dissolved oxygen (DO), ammonia concentration, and pH were measured from the aquaria water, at the start and end of each experiment. After exposure, the shrimp’s hemolymph was sampled for lactate and osmolality assays. Muscle tissue was sampled for hydration content (Muscle Water Content, MWC). Oxygen consumption was not reduced and hemolymph lactate did not increase with increased salinity. The pH of the water decreased with time, under all conditions. Ammonia excretion decreased with increased salinity. Hemolymph osmolality and MWC remained stable at 10 and 20 psu, but osmolality increased (~50%) and MWC decreased (~4%) at 30 psu. The expected reduction in oxygen consumption was not observed. This shrimp is able to tolerate significant changes in water salt concentrations for a few hours by keeping its metabolism in aerobic mode, and putatively shutting down branchial salt uptake to avoid massive salt load, thus remaining strongly hyposmotic. Aerobic metabolism may be involved in the maintainance of cell volume, concomitant with reduced protein/aminoacid catabolism upon increase in salinity. More studies should be conducted to broaden our knowledge on palaemonid hyporegulation.
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