Publicações - ciências biológicas
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ARAÚJO, MAÍRA XAVIER;
Freddy Bravo;
Cláudio J B Carvalho
Palavra-chave:
Biomes;
Moth flies
Áreas do conhecimento:
Ciências Biológicas; Zoologia; Taxonomia dos Grupos Recentes
resumo ...
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Raciele Ivandra Guarda Korelo;
Luis Cláudio Fernandes
Palavra-chave:
ultrasonic therapy;
Cicatrização de Feridas
Áreas do conhecimento:
Ciências da Saúde; Farmacologia; Transmissão Neuromuscular;
Ciências Biológicas; Fisiologia; Fisiologia Geral
resumo ...
(en)
Introduction: the use therapeutic ultrasound (TUS) in wound healing is still discussed by the scientific community. Objetive: to determine whether the UST produces beneficial outcomes in wound healing and the parameters wich have been used to provide better dose-response. Methods: The databases PubMed, SciELO and PEDro were searched. We selected 19 clinical trials, assessed by the PEDro scale and JADAD. Results: of 19 studies, 8 were related to the application of NCLFU (Noncontact Low Frequency Ultrasound) with moderate evidence for its application; 6 were related to the application of HFU (High Frequency Ultrasound) and 1 was related to the application of LIPUS (Low Intensity Pulsed Ultrasound), both of with low evidence. Dose-response relationship there was no agreement between the studies in this review. Conclusion: there is moderate evidence of NCLFU and limited the HFU as an adjunct to wound care resource, not being able to respond clearly the best dose-response relationship.
(pt)
Introdução: o uso do ultrassom terapêutico (UST) para cicatrização de feridas ainda é discutido pela comunidade científica. Objetivo: determinar se o UST produz desfechos benéficos na cicatrização de feridas e quais tem sido os parâmetros utilizados para proporcionar melhor dose-resposta. Métodos: Foram pesquisadas as bases de dados PubMEd, SciELO e PEDro. Foram selecionados 19 ensaios clínicos, avaliados pelas escalas PEDro e JADAD. Resultados: dos 19 estudos, 8 relacionaram-se à aplicação do NCLFU (Noncontact Low Frequency Ultrasound) com evidência moderada para sua aplicação, 6 à aplicação do HFU (High Frequency Ultrasound) e 1 à aplicação de LIPUS (Low Intensity Pulsed Ultrasound), ambos com baixa evidência. Quanto a relação dose-resposta, não há concordância entre os estudos desta revisão. Conclusões: existe evidência moderada da NCLFU e limitada da HFU como recurso adjuvante para o tratamento de feridas, não sendo possível responder com clareza a melhor relação dose-resposta.
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PYRCZ, T W;
LORENC-BRUDECKA, J;
FREITAS, A V L;
BOYER, P;
DIAS, F M S;
DOLIBAINA, D R;
BARBOSA, E P;
MAGALDI, L M;
MIELKE, O H H;
Mirna Martins Casagrande
Palavra-chave:
molecular phylogeny;
morphology;
Pronophilina;
Taxonomy
Áreas do conhecimento:
Ciências Biológicas; Entomologia;
Ciências Biológicas; Zoologia; Taxonomia dos Grupos Recentes; Sistematica Bionomia e Biodiversidade;
Ciências Biológicas; Zoologia; Taxonomia dos Grupos Recentes; Sistemática e taxonomia de Lepidoptera
resumo ...
The genus Praepedaliodes Forster, 1964, the only representative of the mega-diverse mostly Andean Pedaliodes complex lineage in the Brazilian Atlantic Forest, is revised. Prior to this study, four species were known, P. phanias (Hewitson, 1862), P. granulata (Butler, 1868), P. amussis (Thieme, 1905) and P. exul (Thieme, 1905). Here, a further six are described, all from SE Brazil, expanding to 10 the number of species in this genus. Lectotypes are designated for P. phanias, P. granulata and P. amussis. The genus is most diverse in the Serra da Mantiqueira (São Paulo, Rio de Janeiro, Minas Gerais) and in the Serra Geral (Paraná, Santa Catarina) with seven species occurring in both ranges. Praepedaliodes phanias is the most widespread species and the only one found in the western part of the Atlantic Forest; only this species and P. duartei Dias, Dolibaina & Pyrcz n. sp. occurring to near sea level. Other species, P. zaccae Dolibaina, Dias & Pyrcz n. sp., P. francinii Freitas & Pyrcz n. sp., P. sequeirae Pyrcz, Dias & Dolbaina n. sp., P. landryi Pyrcz & Freitas n. sp. and P. pawlaki Pyrcz & Boyer n. sp. are strictly montane and the highest species richness is reached at 1400–1800 m. One species, P. sequeirae n. sp., is a narrow endemic found only at timberline in the Agulhas Negras massif above 2300 m. Immature stages are described for two species, P. phanias and P. landryi n. sp. Molecular data (barcode region of cytochrome oxidase, subunit I) and adult morphology, including male and female genitalia, support the genus as monophyletic, belonging to a predominantly Andean clade of the Pedaliodes Butler, 1867 complex. Morphological evidences, in particular female genitalia comparative analysis, indicate the genera Physcopedaliodes Forster, 1964 and Panyapedaliodes Forster, 1964 as possibly the closest relatives to Praepedaliodes. Molecular data are inconclusive in this respect.
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Ângelo Parise Pinto;
Gabriel Luis Figueira Mejdalani;
Daniela Maeda Takiya
Palavra-chave:
Cicadellinae;
Auchenorrhyncha;
leafhopper;
Leucopepla;
Neotropical region;
Taxonomy
resumo ...
(en)
The taxonomy of the Proconiini genus Diestostemma Amyot & Serville is revisited and the D. bituberculatum species complex is proposed to include D. bituberculatum (Signoret), D. rubriventris (Schmidt), and four new species. A revision of this species complex includes a new geographical record for D. rubriventris and description and illustration of three Ecuadorian and one Brazilian new species based on males and females: D. albinoi sp. nov. (Ecuador: Orellana Province), D. cavichiolii sp. nov. (Brazil: Mato Grosso State), D. gervasioi sp. nov. (Ecuador: Orellana Province), and D. olivia sp. nov. (Ecuador: Orellana Province). These species, all named as homages to the treehopper specialist Dr. Albino Morimasa Sakakibara and his former students, are very similar to D. bituberculatum in general appearance, differing mainly by the shape of the male and female terminalia, but also by slight aspects of the external morphology. The status of the name-bearing specimen of D. rubriventris is modified from lectotype to holotype. The study shows that the species diversity of Diestostemma is far from being entirely known. However, several old available names should be evaluated in a full revision context before description of other new species.
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ORTIZ-ACEVEDO, ELENA;
BONFANTTI, DAYANA;
Mirna Martins Casagrande;
MIELKE, OLAF HERMAN HENDRIK;
ESPELAND, MARIANNE;
WILLMOTT, KEITH R;
MUTANEN, MARKO
Palavra-chave:
Preponini;
Phylogeny;
Taxonomy;
morphology;
DNA
Áreas do conhecimento:
Ciências Biológicas; Entomologia;
Ciências Biológicas; Zoologia; Taxonomia dos Grupos Recentes; Sistematica Bionomia e Biodiversidade
resumo ...
Abstract. The nymphalid tribe Preponini (Lepidoptera) contains some of the most highly visible and easily recognized Neotropical butterflies, yet work is still
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PREDIGER, KAROLINE C.;
Cyntia Maria Telles Fadel-Picheth;
SUREK, MONICA;
DALLAGASSA, CIBELLE B.;
ASSIS, FLÁVIA E.A.;
PIANTAVINI, MARIO S.;
Emanuel Maltempi de Souza;
Fábio de Oliveira Pedrosa;
FARAH, SÔNIA M.S.S.;
ALBERTON, DAYANE
Palavra-chave:
Aeromonas;
carbon source;
Biolog;
mucin
Áreas do conhecimento:
Ciências Biológicas; Microbiologia; Biologia e Fisiologia dos Microorganismos; Bacteriologia;
Ciências Biológicas; Microbiologia; Biologia e Fisiologia dos Microorganismos; Virologia
resumo ...
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PREDIGER, KAROLINE C.;
Fadel-Picheth, Cyntia M.T.;
SUREK, MONICA;
DALLAGASSA, CIBELLE B.;
ASSIS, FLÁVIA E.A.;
PIANTAVINI, MARIO S.;
Emanuel Maltempi de Souza;
Fábio de Oliveira Pedrosa;
FARAH, SÔNIA M.S.S.;
KRIEGER, NADIA
Palavra-chave:
Aeromonas;
carbon source;
Biolog;
mucin
Áreas do conhecimento:
Ciências Biológicas; Microbiologia; Biologia e Fisiologia dos Microorganismos; Bacteriologia;
Ciências Biológicas; Microbiologia; Biologia e Fisiologia dos Microorganismos; Virologia
resumo ...
Bacteria in the genus Aeromonas are primarily aquatic organisms; however, some species can cause diseases in humans, ranging from wound infections to septicemia, of which diarrhea is the most commo...
Les bactéries du genre Aeromonas sont des organismes principalement aquatiques; toutefois, certaines espèces peuvent causer des maladies chez l’humain, allant d’infections de lésions à la septicémi...
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ZANNI, PÂMELA CRISTINA MASTELLARO DELVAS;
BONFIM-MENDONÇA, PATRICIA DE SOUZA;
NEGRI, MELYSSA;
NAKAMURA, SANDRA SAYURI;
Donatti, Lucélia;
Terezinha Inez Estivalet Svidzinski;
CONSOLARO, MARCIA EDILAINE LOPES
Palavra-chave:
Candida albicans
Áreas do conhecimento:
Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia;
Ciências Biológicas; Microbiologia; Biologia e Fisiologia dos Microorganismos; Virologia
resumo ...
ABSTRACT Vulvovaginal candidiasis (VVC) in HIV-infected (HIV+) women is a serious public health...
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SANTOS, K. F. D. N.;
Steffens, M. B. R.;
MOURE, V. R.;
HAUER, V.;
SANTOS, A. R. S;
Donatti, Lucélia;
GALVÃO, C. W.;
PEDROSA, F. O.;
SOUZA, E. M.;
WASSEM, R.
Palavra-chave:
Azospirillum brasilense
Áreas do conhecimento:
Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia
resumo ...
In this work, an ammonium-excreting strain (HM053) of A. brasilense was further characterized genetically and biochemically, and its abilities to colonize and promote wheat growth were determined. Immunoblot, reverse transcription-qPCR, and DNA sequencing were used for HM053 characterization. To analyze wheat-A. brasilense interaction nifH::gusA fusions in the wild-type FP2 (FP2-7) and HM053 (HM053-36) backgrounds were employed. HM053 glutamine synthetase (GS) was not adenylylated in response to an ammonium shock or under any condition tested. Sequencing of the glnA gene revealed a substitution of a proline residue by a leucine at position 347 of the GS. Under axenic growth condition, HM053 was capable of colonizing the surface of wheat roots and increased by 30 and 49% the shoot and root dry weight, respectively, when compared with uninoculated plants, and by 30 and 31% when compared with the parental strain FP2. Although HM053-36 and FP2-7 showed GUS activity located mainly at lateral root emergence points, HM053-36 consistently showed stronger signals and expressed the nifH gene at a level 278 fold higher than strain FP2 in planta, according to qPCR data. HM053, a spontaneous mutant in GS, increased wheat root and shoot dry weight when compared to the wild-type FP2. HM053 ability to excrete ammonium and fix nitrogen constitutively, even in the presence of high NH4
+ concentration, could explain why this mutant has a higher potential to promote plant growth than FP2 and suggests HM053 as a potential nitrogen biofertilizer. However, HM053 should be tested under field conditions to evaluate its abilities to compete with indigenous microflora.
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Taiomara Butkze Dapper;
Vanessa Cristina Arfelli;
Caroline Henn;
Márcia Regina Simões;
Marise Fonseca dos Santos;
Carla Lieko Della Torre;
José Luis da Conceição Silva;
Rita de Cássia Garcia SImão;
Marina Kimiko Kadowaki
Palavra-chave:
Aspergillus;
factorial design;
fruit waste;
invertase
Áreas do conhecimento:
Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia;
Ciências Biológicas; Biotecnologia; Biotecnologia Industrial
resumo ...
This study explores the production and characterization of an extracellular β-fructofuranosidase (FFase-I) by Aspergillus versicolor newly isolated from Atlantic Forest-Brazil. The β-fructofuranosidase production by fungus, after the optimization process using central composite design and response surface methodology, showed that 3% (w/v) apple pomace, an initial pH 7.5, and 12 days of cultivation provided the best conditions. The β-fructofuranosidase (FFase-I) was purified from the crude extract by 75% (NH4)2SO4 precipitation followed by DEAE-Sephadex, and the molecular mass of the FFase-I was estimated to be 75 kDa by SDS-PAGE. Furthermore, the enzyme exhibited unusual tolerance to Cu2+, sodium dodecyl sulfate (SDS), Tween 80, ethanol and acetone. The purified enzyme had an optimum pH of 6.0 and was stable over an acidic pH range of 3.0–6.0. The optimum temperature of the FFase-I was 55°C but was stable at 60°C for 7 h. Thus, the β-fructofuranosidase A. versicolor which has thermal stability and activity under acidic conditions would have potential application in sugar cane molasses hydrolysis for subsequent ethanol production.
Key words: Aspergillus, factorial design, fruit wastes, invertase.
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