Publicações - ciências biológicas
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Silvia Almeida dos Santos;
André Padial;
Eric de Camargo Smidt;
L. L. F. RIBAS
Palavra-chave:
Endangered species;
conservation;
germination rate;
Leaf explant;
culture medium;
Micropropagation
Áreas do conhecimento:
Ciências Biológicas; Fisiologia Vegetal
resumo ...
Seed storage method for in vitro germination and propagation from leaves of Brasiliorchis picta was developed. Seeds were harvested and stored at -20 and -80°C for 1, 3, 6, and 12 months and were germinated on Knudson C (KC), Murashige and Skoog (MS), half-strength MS (½ MS macro- and micro-nutrients), and woody plant medium (WPM). Seeds stored at -20°C, the recommended temperature for seed banks, had a high germination rate (76.0%) when cultivated in WPM after 12 months of storage. WPM is the best medium for seed germination and seedling development for both harvested and stored seeds, regardless of storage time and storage temperature. Whole leaf and leaf transversal thin cell layers (tTCL) from 3-month-old in vitro grown protocorms were cultured in ½ MS supplemented with 6-benzyladenine (BA; 2.5, 5.0 and 10.0 µM) and thidiazuron (TDZ; 3.0, 6.0 and 9.0 µM) for 12 weeks. The highest frequency of regenerated protocorm-like bodies (PLBs) from explants (70.0%) occurred when whole leaves were cultured in medium containing 5.0 µM Ba, whereas the best response for leaf TCL was with the basal section in medium containing 9 µM TDZ, in which PLBs developed in all regions of leaves. Plantlets were successfully acclimatized (with a survival rate of 97%) when vermiculite was used as a substrate.
Key words: Endangered species, conservation, germination rate, leaf explant, culture medium, micropropagation, Orchidaceae, thin cell layer.
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SILVA, C.B.;
MIGUEL, M.D.;
OLIVEIRA, M.;
DIAS, J.F.;
ZANIN, S.M.W.;
Gedir de Oliveira Santos;
CÂNDIDO, A.C.S.;
PERES, M.T.L.P.;
Euclésio SIMIONATTO;
MIGUEL, O.G.
Palavra-chave:
Estresse Oxidativo;
lixiviação;
fitoherbicida
Áreas do conhecimento:
Ciências Biológicas; Fisiologia Vegetal
resumo ...
RESUMO O objetivo desse trabalho foi avaliar a atividade alelopática dos lixiviados das raízes e...
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PEREIRA, GABRIELY PINTO;
Flavio Zanette;
Luis Antonio Biasi;
Ruy Inácio Neiva de Carvalho
Ciência Florestal (UFSM. Impresso),
v. 26,
n. 1,
p. 203-211,
2016
Palavra-chave:
Araucaria;
Tetrazólio
Áreas do conhecimento:
Ciências Biológicas; Fisiologia Vegetal
resumo ...
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SCHERER, MARISA D.;
M. C. Perreira;
André Bellin Mariano;
Jóse Viriato Coelho Vargas
Palavra-chave:
Análise de ciclo de vida;
floculação
resumo ...
(en)
In this article were evaluated flocculation and environmental efficiency of flocculation agents: iron sulfate (FeCl3), sodium hydroxide (NaOH), calcium hydroxide (Ca(OH)2) and aluminum sulfate (Al2(SO4)3). For the flocculation efficiency analysis tests were conducted to determine the optimal concentration of each flocculant agent for Scenedesmus sp species., and the environmental assessment was conducted a Life Cycle Assessment (LCA) with the help of the software SimaPro 7.3 using CML method 2000. The results indicated, that the FeCl3 and Al2 (SO4)3 were highly effective for the recovery of microalgal biomass and they had lower environmental impact, however, when using the FeCl3 there was an immediate change of biomass coloring After addition of salt to a strong shade of orange, which can be associated with the iron present and the use of Al2(SO4)3 may compromise the use of biomass due to some authors associates it with the occurrence of Alzheimer's disease, being restricted the application of biomass recovered through this process for nutritional purposes, therefore, for this work it was noted that the NaOH had lower emissions in virtually all categories examined CML 2000 except for abiotic depletion and terrestrial ecotoxicity, however, was considered the standard flocculating agent such analyzes, since coagulation promoted as efficient values as 93.5% and with better environmental profile between which flocculating agents caused no visible color change in the biomass, together with the coupling technologies flocculation and centrifugation to obtain wet biomass and flocculation and thermal drying to dry biomass. The results show that it is important to consider both aspects of effectiveness of flocculation and environmental impacts to identify the best coagulants in industrial scale. However, further studies are necessary with natural coagulants such as chitosan which is manufactured from fishing waste being an alternative to improve the environmental profile of the process, as well as the use of tannin, however, these suggestions have to be analyzed in future.
(pt)
Neste artigo foram avaliados a eficiência de floculação e ambiental dos agentes floculantes: sulfato de ferro (FeCl3), hidróxido de sódio (NaOH), hidróxido de cálcio (Ca(OH)2) e sulfato de alumínio (Al2(SO4)3). Para a análise de eficiência de floculação foram realizados testes para determinar a melhor concentração de cada agente floculante para a espécie Scenedesmus sp., e para a avaliação ambiental foi realizado a Avaliação do Ciclo de Vida (ACV) com auxílio do software SimaPro 7.3 utilizando o método CML 2000. Os resultados indicaram que o FeCl3 e Al2(SO4)3 foram altamente eficazes para a recuperação da biomassa microalgal e os mesmos apresentaram menor impacto ambiental, porém, ao se utilizar o FeCl3 verificou-se a imediata mudança de coloração da biomassa após adição do sal para um tom forte de laranja, o qual pode ser associado à presença do ferro, e o uso do Al2(SO4)3 pode comprometer o uso da biomassa devido alguns autores associa-lo com a ocorrência da doença de Alzheimer, ficando restrita a aplicação da biomassa recuperada através deste processo para fins nutricionais, portanto, para esse trabalho notou-se que o NaOH apresentou menores emissões em praticamente todas as categorias analisadas do CML 2000, exceto para depleção abiótica e ecotoxicidade terrestre, no entanto, foi considerado o agente floculante padrão dessas análises, uma vez que promoveu a coagulação com valores tão eficientes quanto 93,5% e com o melhor perfil ambiental entre os agentes floculantes que não causaram alterações visíveis de cor na biomassa, juntamente com o acoplamento de tecnologias de floculação e centrifugação para obtenção da biomassa úmida e floculação e secagem térmica para biomassa seca. Os resultados mostram que é importante considerar tanto os aspectos de eficiência da floculação quanto impactos ambientais para identificar os melhores coagulantes em escala industrial. No entanto, mais estudos são necessários com coagulantes naturais, como a quitosana que é fabricado a partir de resíduos de pesca sendo uma alternativa para melhorar o perfil ambiental do processo, assim como a utilização do tanino, entretanto, essas sugestões precisam ser analisadas futuramente.
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SCHERER, MARISA D.;
M. C. Perreira;
André Bellin Mariano;
Jóse Viriato Coelho Vargas
RGSA: Revista de Gestão Social e Ambiental,
v. 5,
n. 1,
p. 92-118,
2016
Palavra-chave:
Análise de ciclo de vida;
floculação
resumo ...
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ALVES, LUIS P. S.;
MÜLLER-SANTOS, MARCELO;
TEIXEIRA, CÍCERO S.;
TIRAPELLE, EVANDRO F.;
Donatti, Lucélia;
TADRA-SFEIR, MICHELLE Z.;
STEFFENS, MARIA B. R.;
DE SOUZA, EMANUEL M.;
DE OLIVEIRA PEDROSA, FABIO;
CHUBATSU, LEDA S.
Palavra-chave:
Herbaspirillum seropedicae
Áreas do conhecimento:
Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia;
Ciências Biológicas; Morfologia
resumo ...
Phasins are important proteins controlling poly-3-hydroxybutyrate (PHB) granules formation, their number into the cell and stability. The genome sequencing of the endophytic and diazotrophic bacterium Herbaspirillum seropedicae SmR1 revealed two homologous phasin genes. To verify the role of the phasins on PHB accumulation in the parental strain H. seropedicae SmR1, isogenic strains defective in the expression of phaP1, phaP2 or both genes were obtained by gene deletion and characterized in this work. Despite of the high sequence similarity between PhaP1 and PhaP2, PhaP1 is the major phasin in H. seropedicae, since its deletion reduced PHB accumulation by ≈50% in comparison to the parental and ΔphaP2. Upon deletion of phaP1, the expression of phaP2 was sixfold enhanced in the ΔphaP1 strain. The responsive backup expression of phaP2 partially rescued the ΔphaP1 mutant, maintaining about 50% of the parental PHB level. The double mutant ΔphaP1.2 did not accumulate PHB in any growth stage and showed a severe reduction of growth when glucose was the carbon source, a clear demonstration of negative impact in the fitness. The co-occurrence of phaP1 and phaP2 homologous in bacteria relatives of H. seropedicae, including other endophytes, indicates that the mechanism of phasin compensation by phaP2 expression may be operating in other organisms, showing that PHB metabolism is a key factor to adaptation and efficiency of endophytic bacteria.
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ALVES, LUIS P. S.;
Marcelo Muller dos Santos;
TEIXEIRA, CÍCERO S.;
TIRAPELLE, EVANDRO F.;
DONATTI, LUCÉLIA;
TADRA-SFEIR, MICHELLE Z.;
STEFFENS, M;
DE SOUZA, EMANUEL M.;
DE OLIVEIRA PEDROSA, FABIO;
Leda Satie Chubatsu
Palavra-chave:
Herbaspirillum seropedicae;
PHB;
PHB metabolism
Áreas do conhecimento:
Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia;
Ciências Biológicas; Bioquímica; Biologia Molecular
resumo ...
Phasins are important proteins controlling poly-3-hydroxybutyrate (PHB) granules formation, their number into the cell and stability. The genome sequencing of the endophytic and diazotrophic bacterium Herbaspirillum seropedicae SmR1 revealed two homologous phasin genes. To verify the role of the phasins on PHB accumulation in the parental strain H. seropedicae SmR1, isogenic strains defective in the expression of phaP1, phaP2 or both genes were obtained by gene deletion and characterized in this work. Despite of the high sequence similarity between PhaP1 and PhaP2, PhaP1 is the major phasin in H. seropedicae, since its deletion reduced PHB accumulation by ≈50% in comparison to the parental and ΔphaP2. Upon deletion of phaP1, the expression of phaP2 was sixfold enhanced in the ΔphaP1 strain. The responsive backup expression of phaP2 partially rescued the ΔphaP1 mutant, maintaining about 50% of the parental PHB level. The double mutant ΔphaP1.2 did not accumulate PHB in any growth stage and showed a severe reduction of growth when glucose was the carbon source, a clear demonstration of negative impact in the fitness. The co-occurrence of phaP1 and phaP2 homologous in bacteria relatives of H. seropedicae, including other endophytes, indicates that the mechanism of phasin compensation by phaP2 expression may be operating in other organisms, showing that PHB metabolism is a key factor to adaptation and efficiency of endophytic bacteria.
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Rafael Anzanello;
Luis Antonio Biasi
Semina. Ciências Agrárias (Online),
v. 37,
n. 4,
p. 1811-1826,
2016
Palavra-chave:
FRUTICULTURA;
dormência
Áreas do conhecimento:
Ciências Biológicas; Fisiologia Vegetal
resumo ...
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Renato Goldenberg;
Lucas de Freitas Bacci;
Thuane Bochorny
resumo ...
Resumo A família Melastomataceae possui 22 gêneros e 150 espécies no estado do Paraná....
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Yamamoto, Flávia Yoshie;
Cestari, M.M.;
FILIPAK NETO, FRANCISCO;
Julio Azevedo;
C A Oliveira Ribeiro;
Monica Pereira;
Elisangela Lottermann;
Gustavo Souza Santos;
Tatiiana Stremel;
DORIA, H.B.;
Paloma Kachel Gusso-Choueri;
Sandro X. Campos;
Claudia Feijó Ortolani Machado
Palavra-chave:
Genotoxicidade;
peixe;
Lambari;
Acará;
Toxicidade
Áreas do conhecimento:
Ciências Biológicas; Farmacologia; toxicidade em peixes;
Ciências Biológicas; Genética; Genética Animal; Mutagênese Ambiental
resumo ...
The Iguaçu River, located at the Southern part of Brazil, has a great socioeconomic and environmental importance due to its high endemic fish fauna and its potential to generate hydroelectric power. However, Iguaçu River suffers intense discharge of pollutants in the origin of the river. In a previous report, the local environmental agency described water quality to improve along the river course. However, no study with integrated evaluation of chemical analysis and biological responses has been reported so far for the Iguaçu River. In the current study, three different Brazilian fish species (Astyanax bifasciatus, Chrenicicla iguassuensis, and Geophagus brasiliensis) were captured in the five cascading reservoirs of Iguaçu River for a multi-biomarker study. Chemical analysis in water, sediment, and muscle indicated high levels of bioavailable metals in all reservoirs. Polycyclic aromatic hydrocarbons (PAHs) were detected in the bile of the three fish species. Integration of the data through a FA/PCA analysis demonstrated the poorest environmental quality of the reservoir farthest from river’s source, which is the opposite of what has been reported by the environmental agency. The presence of hazardous chemicals in the five reservoirs of Iguaçu River, their bioaccumulation in the muscle of fish, and the biological responses showed the impacts of human activities to this area and did not confirm a gradient of pollution between the five reservoirs, from the source toward Iguaçu River’s mouth. Therefore, diffuse source of pollutants present along the river course are increasing the risk of exposure to biota and human populations.
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