Publicações - ciências biológicas
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KITA, DIOGO H.;
MEYER, KATLYN B.;
VENTURELLI, AMANDA C.;
ADAMS, RAFAELLA;
MACHADO, DARIA L.B.;
Morais, Rosana N.;
SWAN, SHANNA H.;
GENNINGS, CHRIS;
Anderson Joel Martino Andrade
Palavra-chave:
phthalates;
anogenital distance;
flutamide;
testosterone;
rats;
reproductive toxicology
resumo ...
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Ana Marcia Delattre Zocolotti;
LIMA, MARCELO M. S.;
ANETE FERRAZ;
Bruno Carabelli;
Marco A. Mori;
KEMPE, PAULA G.;
RIZZO DE SOUZA, LUIZ E.;
Silvio Marques Zanata;
Machado, Ricardo B.;
Suchecki, Deborah;
ANDRADE DA COSTA, BELMIRA L. S.
Palavra-chave:
ácidos graxos;
Doença de Parkinson;
polyunsaturated fatty acids
Áreas do conhecimento:
Ciências Biológicas; Fisiologia; Neurociências;
Ciências Biológicas; Bioquímica; Química de Macromoléculas; Lipídeos;
Ciências Biológicas; Fisiologia; Fisiologia Geral
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Evidence suggests that idiopathic Parkinson’s disease (PD) is the consequence of a neurodevelopmental disruption, rather than strictly a consequence of aging. Thus, we hypothesized that maternal supplement of omega-3 polyunsaturated fatty acids (ω-3 PUFA) may be associated with neuroprotection mechanisms in a self-sustaining cycle of neuroinflammation and neurodegeneration in lipopolysaccharide (LPS)-model of PD. To test this hypothesis, behavioral and neurochemical assay were performed in prenatally LPS-exposed offspring at postnatal day 21. To further determine whether prenatal LPS exposure and maternal ω-3 PUFAs supplementation had persisting effects, brain injury was induced on PN 90 rats, following bilateral intranigral LPS injection. Pre- and postnatal inflammation damage not only affected dopaminergic neurons directly, but it also modified critical features, such as activated microglia and astrocyte cells, disrupting the support provided by the microenvironment. Unexpectedly, our results failed to show any involvement of caspase-dependent and independent apoptosis pathway in neuronal death mechanisms. On the other hand, learning and memory deficits detected with a second toxic exposure were significantly attenuated in maternal ω-3 PUFAs supplementation group. In addition, ω-3 PUFAs promote beneficial effect on synaptic function, maintaining the neurochemical integrity in remaining neurons, without necessarily protect them from neuronal death. Thus, our results suggest that ω-3 PUFAs affect the functional ability of the central nervous system in a complex way in a multiple inflammation-induced neurotoxicity animal model of PD and they disclose new ways of understanding how these fatty acids control responses of the brain to different challenges.
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Fabián A. Michelangeli;
Renato Goldenberg
Áreas do conhecimento:
Ciências Biológicas; Taxonomia Vegetal; Taxonomia de Fanerógamos
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Miconia papillosperma, a new species of Melastomataceae shrubs from Northern Brazil is described and illustrated. This new species is characterized by elliptic lanceolate leaves with the only pair of secondary veins running close to the margin. It is also unique in having seeds with a papillose testa, a character until now unknown in the Miconieae. The description of this new species from a relatively well collected area near a major road north of Manaus, Amazonas, Brazil, is further evidence of our lack of knowledge on plants in many Neotropical areas.
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Yohana de Oliveira-Cauduro;
Valéria Rosa Lopes;
CLAUDINE MARIA DE BONA;
GIOVANA BOMFIM DE ALCANTARA;
Luis Antonio Biasi
Plant Cell Culture & Micropropagation,
v. 12,
p. 53-60,
2016
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DOS SANTOS, EMILI BORTOLON;
FAVRETTO, MARIO ARTHUR;
DOS SANTOS COSTA, SAMUEL GEREMIAS;
Mário Antonio NavarroSilva
Palavra-chave:
Arrenurus;
Acari;
Culicidae;
Mata Atlântica
Áreas do conhecimento:
Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia;
Ciências Biológicas; Parasitologia
resumo ...
In this study, a total of 4146 culicids collected in an Atlantic Forest area in Paraná state, southern Brazil were examined for the presence of mites. Forty larval Parasitengone mites (Arrenurus spp., Arrenuridae; Durenia spp., Trombellidae; Microtrombidium spp., Microtrombidiidae) parasitized 25 specimens of mosquitoes, with the intensity varying from one to nine mites attached. Most mites were found on Aedes serratus/nubilus, Culex vomerifer, Cx. pedroi and Cx. sacchettae. The overall percentage of parasitized mosquitoes was 0.6 %. The highest intensity of mites encountered was in an individual of Cx. pedroi with nine attached mites. Regarding the attachment site, most mite specimens were attached to the abdomen (n = 25), whereas 15 were located on the thorax. Specimens of Arrenurus spp. were only found on the abdomen of mosquitoes, and the same was observed for Microtrombidium spp., while Durenia spp. attached to both the thorax (n = 15) and abdomen (n = 4). This is the first record for the genus Durenia in Brazil. Additionally, some species of mosquitoes were, for the first time, reported as being parasitized by mites.
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Fabiane Oliveira Farias;
Aline Alberti;
Alessandro Nogueira;
Ivo Motin Demiate
Palavra-chave:
by-products;
Experimental design;
fermentation kinetics;
sodium monensin
resumo ...
(en)
Apart from creating many positive impacts, the growth of agribusiness has also generated concerns about the production of large amounts of undervalued by-products. Cheese whey and corn steep liquor (from corn flour production, a typical Brazilian food) are among these by-products that can be potential raw materials for obtaining new products such as bioethanol. The objective of this study was to apply an experimental design tool to examine different mixtures of cheese whey and corn steep liquor for the production of bioethanol. Between the worts studied, those containing 100 % corn steep liquor, and 25 % cheese whey with 75 % corn steep liquor supplemented with glucose were considered the best for the production of bioethanol, with fermentation efficiency reaching around 90 % for both of them. The use of pasteurization and antibiotics was effective in controlling lactic acid production, which results from bacterial contamination. The use of antibiotics showed advantages by reducing the maximum growth rate of yeast, which was reflected in higher rates of fermentation efficiency. The worts proposed allowed reaching ethanol productivity of up to 1.93 g L-1 h-1.
(pt)
Apart from creating many positive impacts, the growth of agribusiness has also generates concerns about the production of large amounts of undervalued by-products. Cheese whey and corn steep liquor (from corn flour production, a typical Brazilian food) are among these by-products that can be potential raw materials for obtaining new products such as bioethanol. The objective of this study was to apply an experimental design tool to examine different worts with cheese whey and corn steep liquor for the production of bioethanol. The worts containing 100 % corn steep liquor, and 25 % cheese whey with 75 % corn steep liquor supplemented with glucose were considered the best for the production of bioethanol, with fermentation efficiency reaching around 90 % for both of them. The use of pasteurization and antibiotics was effective in controlling lactic acid production, which results from bacterial contamination. The use of antibiotics showed advantages by reducing the maximum growth rate of yeast, which was reflected in higher rates of fermentation efficiency. The results obtained allowed reaching a high level of ethanol productivity.
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Jóse Viriato Coelho Vargas;
Vanessa Kava;
Wellington Balmant;
André Bellin Mariano;
ORDONEZ, J.C.
Palavra-chave:
Scenedesmus sp.;
Chlamydomonas reinhardtii;
mathematical modeling;
sustainable energy;
genetically modified organisms (GMO);
well stirred phtobioreactor
Áreas do conhecimento:
Engenharias; Engenharia Térmica; Controle Ambiental;
Ciências Biológicas; Genética; Genética Molecular e de Microorganismos
resumo ...
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OLIVEIRA, MARIA T.A.;
BENTO, RICARDO R.F.;
TEIXEIRA, ALEXANDRE M.R.;
CASSIANO, CÍCERA J.M.;
SENA, DINIZ M.;
COUTINHO, HENRIQUE D.M.;
MENEZES, IRWIN R.A.;
FIGUEREDO, FERNANDO G.;
Luiz Everson da Silva;
TOLEDO, THIAGO A.
Palavra-chave:
antimicrobial agent;
modulation;
microbial resistance
Áreas do conhecimento:
Ciências Biológicas; Farmacologia Bioquímica e Molecular
resumo ...
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MEISSNER, GABRIEL OTTO;
Silvio Sanches Veiga;
OLGA CHAIM;
Pedro Túlio de Resende Lara;
Luis Paulo Barbour Scott;
Antônio Sérgio Kimus Braz;
Daniele Chaves-Moreira;
Fernando Hitomi Matsubara;
Eduardo Mendonça Soares;
Dilza Trevisan-Silva;
Luiza Helena Gremski
Palavra-chave:
Aranha marrom;
Veneno;
ICKs;
knottinas;
modelagem molecular;
Toxina inseticida
Áreas do conhecimento:
Ciências Biológicas; Morfologia; Citologia e Biologia Celular;
Ciências Biológicas; Bioquímica; Biologia Molecular
resumo ...
Inhibitor cystine knots (ICKs) are a family of structural peptides with a large number of cysteine residues that form intramolecular disulfide bonds, resulting in a knot. These peptides are involved in a variety of biological functions including predation and defense, and are found in various species, such as spiders, scorpions, sea anemones, and plants. The Loxosceles intermedia venom gland transcriptome identified five groups of ICK peptides that represent more than 50 % of toxin-coding transcripts. Here, we describe the molecular cloning of U2-Sicaritoxin-Lit2 (U2-SCRTX-Lit2), bioinformatic characterization, structure prediction, and molecular dynamic analysis. The sequence of U2-SCRTX-Lit2 obtained from the transcriptome is similar to that of μ-Hexatoxin-Mg2, a peptide that inhibits the insect Nav channel. Bioinformatic analysis of sequences classified as ICK family members also showed a conservation of cysteine residues among ICKs from different spiders, with the three dimensional molecular model of U2-SCRTX-Lit2 similar in structure to the hexatoxin from μ-hexatoxin-Mg2a. Molecular docking experiments showed the interaction of U2-SCRTX-Lit2 to its predictable target—the Spodoptera litura voltage-gated sodium channel (SlNaVSC). After 200 ns of molecular dynamic simulation, the final structure of the complex showed stability in agreement with the experimental data. The above analysis corroborates the existence of a peptide toxin with insecticidal activity from a novel ICK family in L. intermedia venom and demonstrates that this peptide targets Nav channels.
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Juliana Flávia Ferreira e Silva Paranaiba;
Fabiano Campos Lima;
Dayane Kelly Sabec;
Eugênio Gonçalves de Araújo;
Kleber Fernando Pereira
Áreas do conhecimento:
Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia
resumo ...
Nenhuma característica dos seres vivos é tão primordial quanto a respiração, e os pulmões são os...
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