Publicações - ciências biológicas
|
DOS SANTOS, DEBORA REGINA;
DE OLIVEIRA RIBEIRO, CIRO ALBERTO;
YAMAMOTO, FLAVIA YOSHIE;
Filipak Neto, Francisco;
M. A. F. Randi;
GARCIA, JUAN ESQUIVEL;
COSTA, DANIELE DIETRICH MOURA;
LIEBEL, SAMUEL;
CAMPOS, SANDRO XAVIER;
VOIGT, CARMEN LÚCIA
Palavra-chave:
biomarcadores;
poluição aquática;
Qualidade de Agua;
reservatórios de água
resumo ...
The knowledge concerning associations between chronic chemical exposure and many disorders with complex etiology involving gene–environment interactions is increasing, and new methods must be developed to improve water quality monitoring. The complexity of chemical mixtures in polluted aquatic environments makes the evaluation of toxic potential in those sites difficult, but the use of biomarkers and bioindicators has been recognized as a reliable tool to assess risk of exposure to biota and also the human population. In order to evaluate the use of fish and biomarkers to assess toxic potential and bioavailability of chemicals in human-related hydric resources, an in situ experiment was accomplished in two water reservoirs designated for human supply, which were previously evaluated by the local environmental regulatory agency through a set of physical, chemical, and classical biological parameters. Molecular, biochemical, and morphological biomarkers were performed in caged Oreochromis niloticus kept for 6 months in the studied reservoirs to assess potentially useful biomarkers to evaluate the quality of water for human supply. Chemical analysis of toxic metals in liver and muscle and polycyclic aromatic hydrocarbons (PAHs) in bile was considered to assess the bioavailability of pollutants and highlight human activity impact. The reservoir previously classified by a governmental agency as less impacted presented more risk of exposure to biota. These results were supported by chemical analysis, vitellogenin expression, histopathological findings (gonads, liver, and gills), as well as indicators of neurotoxic effects and oxidative stress in liver. The inclusion of some biomarkers as parameters in regulatory monitoring programs in reservoirs designated for human supply is strongly suggested to evaluate the risks of exposure to the human population. Thus, a revision of the traditional biological and physicochemical analysis utilized to establish the conditions of water quality is necessary.
|
|
KREMER, LAURA PIOLI;
Rosana Moreira da Rocha
Palavra-chave:
ASCIDIACEA;
espécies introduzidas;
Predacao;
Experimento;
gradiente latitudinal;
SUBSTRATO ARTIFICIAL
Áreas do conhecimento:
Ciências Biológicas; Ecologia; Ecologia de Ecossistemas
resumo ...
Predation may often influence native species dynamics and so may be important for the control of introduced species as well. Here, we examine how predation can regulate fouling communities on artificial substrates in the coast of Brazil. Specifically, we tested whether predators limit colonization and establishment of introduced species. A predation experiment using plastic plates as experimental replicates (predator excluded and not excluded) was carried out in Ceará, Bahia, São Paulo and Santa Catarina, between 3°S and 27°S. Ninety-eight species colonized the plates, 14 of which were introduced. While species richness was similar among the treatments (except in Santa Catarina), community structure varied by treatment and introduced species were less abundant in the predation treatment at all sites. Also, predation was selective and controlled introduced, poorly defended, species, specifically ascidians. Thus, biotic resistance driven by predation was not ubiquitous but apparently targets one group of major economic and environmental impact. As a consequence, introduced barnacles were released from competition with dominant ascidians and thus the ecosystem service (biotic resistance) performed by fish alone was unable to completely avoid or control the introduction of non-native species. Control measures must rely on various approaches, but if we want fish to contribute in this control, it is very important to conserve the local native assembly of predators in order to reap their benefits in this ecosystem service.
|
|
Anghebem-Oliveira, Mauren Isfer;
WEBBER, SUSAN;
KRIEGER, NADIA;
Emanuel Maltempi de Souza;
Giseli Klassen;
Geraldo Pichet;
REGO, F.G.M.
Palavra-chave:
gene polymorphism;
Gestational Diabetes
Áreas do conhecimento:
Ciências Biológicas; Biologia Molecular
resumo ...
Background
The glucokinase regulatory protein (GCKR) regulates the activity of the glucokinase (GCK), which plays a key role in glucose homeostasis. Genetic variants in GCK have been associated with...
|
|
Anghebem-Oliveira, Mauren Isfer;
WEBBER, SUSAN;
Dayane Alberton;
Emanuel Maltempi de Souza;
Giseli Klassen;
Geraldo Pichet;
REGO, F.G.M.
Palavra-chave:
gene polymorphism;
Gestational Diabetes
resumo ...
|
|
Lucas de Freitas Bacci;
Mayara Krasinski Caddah;
Renato Goldenberg
Phytotaxa: a rapid international journal for accelerating the publication of botanical taxonomy,
v. 271,
p. 1-92,
2016
DOI
Áreas do conhecimento:
Ciências Biológicas; Taxonomia Vegetal; Taxonomia de Fanerógamos
resumo ...
|
|
Lineu Cesar Werneck;
Paulo José Lorenzoni;
ARNDT, RAQUEL CRISTINA;
CLAUDIA S K KAY;
Scola, Rosana Herminia
Palavra-chave:
neurologia;
imunogenética;
HLA
resumo ...
|
|
Alessandra Biz;
Maura Harumi Sugai-Guérios;
Joosu Kuivanen;
Hannu Maaheimo;
KRIEGER, NADIA;
MITCHELL, DAVID ALEXANDER;
Peter Richard
Palavra-chave:
Saccharomyces cerevisiae;
ethanol;
genetic engineering;
D-galacturonic acid
Áreas do conhecimento:
Engenharias; Engenharia Química; Processos Industriais de Engenharia Química; Processos Bioquímicos;
Ciências Biológicas; Microbiologia; Microbiologia Aplicada; Microbiologia Industrial e de Fermentação
resumo ...
Pectin-rich wastes, such as citrus pulp and sugar beet pulp, are produced in considerable amounts by the juice and sugar industry and could be used as raw materials for biorefineries. One possible process in such biorefineries is the hydrolysis of these wastes and the subsequent production of ethanol. However, the ethanol-producing organism of choice, Saccharomyces cerevisiae, is not able to catabolize d-galacturonic acid, which represents a considerable amount of the sugars in the hydrolysate, namely, 18 % (w/w) from citrus pulp and 16 % (w/w) sugar beet pulp. In the current work, we describe the construction of a strain of S. cerevisiae in which the five genes of the fungal reductive pathway for d-galacturonic acid catabolism were integrated into the yeast chromosomes: gaaA, gaaC and gaaD from Aspergillus niger and lgd1 from Trichoderma reesei, and the recently described d-galacturonic acid transporter protein, gat1, from Neurospora crassa. This strain metabolized d-galacturonic acid in a medium containing d-fructose as co-substrate. This work is the first demonstration of the expression of a functional heterologous pathway for d-galacturonic acid catabolism in Saccharomyces cerevisiae. It is a preliminary step for engineering a yeast strain for the fermentation of pectin-rich substrates to ethanol.
|
|
Alessandra Biz;
Maura Harumi Sugai-Guérios;
Joosu Kuivanen;
Hannu Maaheimo;
KRIEGER, NADIA;
MITCHELL, DAVID ALEXANDER;
Peter Richard
Palavra-chave:
Saccharomyces cerevisiae;
D-galacturonic acid;
genetic engineering
Áreas do conhecimento:
Ciências Biológicas; Microbiologia Aplicada; Microbiologia Industrial e de Fermentação
resumo ...
Pectin-rich wastes, such as citrus pulp and sugar beet pulp, are produced in considerable amounts by the juice and sugar industry and could be used as raw materials for biorefineries. One possible process in such biorefineries is the hydrolysis of these wastes and the subsequent production of ethanol. However, the ethanol-producing organism of choice, Saccharomyces cerevisiae, is not able to catabolize d-galacturonic acid, which represents a considerable amount of the sugars in the hydrolysate, namely, 18 % (w/w) from citrus pulp and 16 % (w/w) sugar beet pulp. In the current work, we describe the construction of a strain of S. cerevisiae in which the five genes of the fungal reductive pathway for d-galacturonic acid catabolism were integrated into the yeast chromosomes: gaaA, gaaC and gaaD from Aspergillus niger and lgd1 from Trichoderma reesei, and the recently described d-galacturonic acid transporter protein, gat1, from Neurospora crassa. This strain metabolized d-galacturonic acid in a medium containing d-fructose as co-substrate. This work is the first demonstration of the expression of a functional heterologous pathway for d-galacturonic acid catabolism in Saccharomyces cerevisiae. It is a preliminary step for engineering a yeast strain for the fermentation of pectin-rich substrates to ethanol.
|
|
Carlos Alberto Cioce Sampaio;
Carlos Alberto Cioce Sampaio;
Cristina de Araújo Lima
Palavra-chave:
Polish migrant architecture;
Araucaria;
Family farmer;
Organization of rural property;
Morphofunctional dynamics
Áreas do conhecimento:
Ciências Biológicas; Multidisciplinarity
resumo ...
|
|
BACHMANN, LUTZ;
FROMM, BASTIAN;
PATELLA DE AZAMBUJA, LUCIANA;
Walter Antonio Boeger
Palavra-chave:
GYRODACTYLIDAE;
DNA mitocondrial;
genoma
resumo ...
The rather species-poor oviparous gyrodactylids are restricted to South America. It was suggested that they have a basal position within the otherwise viviparous Gyrodactylidae. Accordingly, it was proposed that the species-rich viviparous gyrodactylids diversified and dispersed from there. The mitochondrial genome of Aglaiogyrodactylus forficulatus was bioinformatically assembled from next-generation illumina MiSeq sequencing reads, annotated, and compared to previously published mitochondrial genomes of other monogenoidean flatworm species. The mitochondrial genome of A. forficulatus consists of 14,371 bp with an average A + T content of 75.12 %. All expected 12 protein coding, 22 tRNA, and 2 rRNA genes were identified. Furthermore, there were two repetitive non-coding regions essentially consisting of 88 bp and 233 bp repeats, respectively. Maximum Likelihood analyses placed the mitochondrial genome of A. forficulatus in a well-supported clade together with the viviparous Gyrodactylidae species. The gene order differs in comparison to that of other monogenoidean species, with rearrangements mainly affecting tRNA genes. In comparison to Paragyrodactylus variegatus, four gene order rearrangements, i.e. three transpositions and one complex tandem-duplication-random-loss event, were detected. Mitochondrial genome sequence analyses support a basal position of the oviparous A. forficulatus within Gyrodactylidae, and a sister group relationship of the oviparous and viviparous forms.
|
