Guia de fontes

Publicações - ciências biológicas

Brown spider venom toxins rise as useful components to produce novel drugs and a rational therapy for loxoscelism Luiza Helena Gremski; Silvio Sanches Veiga; Fernando Hitomi Matsubara; Bruno Cesar Antunes; Nayanne Louise Costacurta Polli; Pedro Schluga; Hanna Camara Justa; João Minozzo; Ana Carolina M. Wille; Andrea Senff Ribeiro FRONTIERS IN MOLECULAR BIOSCIENCES, v. 8, p. 706704 2021 DOI Palavra-chave: Aranha marrom; Toxinas; Propriedades funcionais; Proteínas recombinantes; Biotecnologia Áreas do conhecimento: Ciências Biológicas; Morfologia; Citologia e Biologia Celular; Ciências Biológicas; Bioquímica; Biologia Molecular resumo ... Brown spider (genus Loxosceles) venoms are mainly composed of protein toxins used for predation and defense. Bites of these spiders most commonly produce a local dermonecrotic lesion with gravitational spread, edema and hemorrhage, which together are defined as cutaneous loxoscelism. Systemic loxoscelism, such as hematological abnormalities and renal injury, are less frequent but more lethal. Some Loxosceles venom toxins have already been isolated and extensively studied, such as phospholipases D (PLDs), which have been recombinantly expressed and were proven to reproduce toxic activities associated to the whole venom. PLDs have a notable potential to be engineered and converted in non-toxic antigens to produce a new generation of antivenoms or vaccines. PLDs also can serve as tools to discover inhibitors to be used as therapeutic agents. Other Loxosceles toxins have been identified and functionally characterized, such as hyaluronidases, allergen factor, serpin, TCTP and knottins (ICK peptides). All these toxins were produced as recombinant molecules and are biologically active molecules that can be used as tools for the potential development of chemical candidates to tackle many medical and biological threats, acting, for instance, as antitumoral, insecticides, analgesic, antigens for allergy tests and biochemical reagents for cell studies. In addition, these recombinant toxins may be useful to develop a rational therapy for loxoscelism. This review summarizes the main ca...
Brown spider venom toxins: what are the functions of astacins, serine proteases, hyaluronidases, allergens, TCTP, serpins and knottins? Luiza Helena Gremski; Fernando Hitomi Matsubara; Silvio Sanches Veiga; Hanna Camara Justa; Zelinda SCHEMCZSSEN; Antonielle Beatriz Baldissera; Pedro Schluga; Isabel Oliveira; Marianna Boia-Ferreira; MARTINS WILLE, ANA CAROLINA; Andrea Senff Ribeiro JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES, v. 27, p. 1-15, 2021 DOI Palavra-chave: Aranha marrom; Veneno; Toxinas; Avaliação biológica; Avaliação bioquímica Áreas do conhecimento: Ciências Biológicas; Morfologia; Citologia e Biologia Celular; Ciências Biológicas; Bioquímica; Biologia Molecular resumo ... Abstract Accidents caused by the bites of brown spiders (Loxosceles) generate a clinical...
Brown Spiders’ Phospholipases-D with Potential Therapeutic Applications: Functional Assessment of Mutant Isoforms Thais Pereira da Silva; Arni, Raghuvir Krishnaswamy; Wille, Ana Carolina Martins; Andrea Senff Ribeiro; Luiza Helena Gremski; Silvio Sanches Veiga; Fernando Jacomini de Castro; Larissa Vuitika; Nayanne Louise Costacurta Polli; Bruno Cesar Antunes; Mariana Bóia-Ferreira; Joaõ Carlos Minozzo; Ricardo Barros Mariutti; Fernando Hitomi Matsubara Biomedicines, v. 9, p. 320 2021 DOI Palavra-chave: Loxosceles; Veneno; fosfolipase-D; Mutação sítio dirigida; Avaliação bioquímica; Avaliação biológica Áreas do conhecimento: Ciências Biológicas; Morfologia; Citologia e Biologia Celular; Ciências Biológicas; Bioquímica; Biologia Molecular resumo ... Phospholipases-D (PLDs) found in Loxosceles spiders’ venoms are responsible for the dermonecrosis triggered by envenomation. PLDs can also induce other local and systemic effects, such as massive inflammatory response, edema, and hemolysis. Recombinant PLDs reproduce all of the deleterious effects induced by Loxosceles whole venoms. Herein, wild type and mutant PLDs of two species involved in accidents—L. gaucho and L. laeta—were recombinantly expressed and characterized. The mutations are related to amino acid residues relevant for catalysis (H12-H47), magnesium ion coordination (E32-D34) and binding to phospholipid substrates (Y228 and Y228-Y229-W230). Circular dichroism and structural data demonstrated that the mutant isoforms did not undergo significant structural changes. Immunoassays showed that mutant PLDs exhibit conserved epitopes and kept their antigenic properties despite the mutations. Both in vitro (sphingomyelinase activity and hemolysis) and in vivo (capillary permeability, dermonecrotic activity, and histopathological analysis) assays showed that the PLDs with mutations H12-H47, E32-D34, and Y228-Y229-W230 displayed only residual activities. Results indicate that these mutant toxins are suitable for use as antigens to obtain neutralizing antisera with enhanced properties since they will be based on the most deleterious toxins in the venom and without causing severe harmful effects to the animals in which these sera are produced.
Cannabidiol induces antidepressant and anxiolytic¿like effects in experimental type-1 diabetic animals by multiple sites of action Yane Costa Chaves; karina genaro; CRIPPA, JOSÉ ALEXANDRE; Cunha, Joice M.; Janaína Menezes Zanoveli Metabolic Brain Disease, v. 36, p. 639-652, 2021 DOI Palavra-chave: cannabidiol; diabetes; streptozotocin; Anxiety Áreas do conhecimento: Ciências Biológicas; Farmacologia; Neuropsicofarmacologia resumo ... Cannabidiol (CBD), a phytocannabinoid compound, presents antidepressant and anxiolytic-like effects in the type-1 diabetes mellitus(DM1) animal model. Although the underlying mechanism remains unknown, the type-1A serotonin receptor (5-HT1A) and cannabinoids type-1 (CB1) and type-2 (CB2) receptors seem to play a central role in mediating the beneficial effects on emotional responses. We aimed to study the involvement of these receptors on an antidepressant- and anxiolytic-like effects of CBD and on some parameters of the diabetic condition itself. After 2 weeks of the DM1 induction in male Wistar rats by streptozotocin (60 mg/kg; i.p.), animals were treated continuously for 2-weeks with the 5-HT1A receptor antagonist WAY100635 (0.1 mg/kg, i.p.), CB1 antagonist AM251 (1 mg/kg i.p.) or CB2 antagonist AM630 (1 mg/kg i.p.) before the injection of CBD (30 mg/kg, i.p.) or vehicle (VEH, i.p.) and then, they were submitted to the elevated plus-maze and forced swimming tests. Our findings show the continuous treatment with CBD improved all parameters evaluated in these diabetic animals. The previous treatment with the antagonists − 5-HT1A, CB1, or CB2 - blocked the CBD-induced antidepressant-like effect whereas only the blockade of 5-HT1A or CB1 receptors was able to inhibit the CBD-induced anxiolytic-like effect. Regarding glycemic control, only the blockade of CB2 was able to inhibit the beneficial effect of CBD in reducing the glycemia of diabetic animals. These findings indicated a therapeutic potential for CBD in the treatment of depression/anxiety associated with diabetes pointing out a complex intrinsic mechanism in which 5-HT1A, CB1, and/or CB2 receptors are differently recruited.
Cannabinoid Receptor 1 and 2 Signalling Pathways Involved in Sepsis LEITE-AVALCA, MARIANE C.G.; Aleksander Roberto Zampronio; LEHMANN, CHRISTIAN SHOCK, v. Publish Ahead of Print, p. Online 2021 DOI Palavra-chave: sepsis; endocanabinóides resumo ... ted immune response in sepsis. Therefore, a better understanding of the pathophysiological mechanisms may be useful for elucidating the molecular basis of sepsis and may contribute to the development of new therapeutic strategies. The endocannabinoid system (ECS) is an emerging research topic for the modulation of the host immune response under various pathological conditions. Cannabinoid receptors include the cannabinoid type 1 receptor (CB1) and the cannabinoid type 2 receptor (CB2). This review addresses the main functionality of CB1 and CB2 in sepsis, which can contribute to a better understanding about the pathophysiology of sepsis. Specifically, we discuss the role of CB1 in the cardiovascular system which is one of the biological systems that are strongly affected by sepsis and septic shock. We are also reviewing the role of CB2 in sepsis, specially CB2 activation, which exerts anti-inflammatory activities with potential benefit in sepsis. Address reprint requests to Mariane C.G. Leite-Avalca, Dalhousie University Faculty of Science Halifax, NS Canada. E-mail: marianeguttervill@gmail.com Received 30 November, 2020 Revised 16 December, 2020 Accepted 12 February, 2021 The authors declare that no competing interests exists. © 2021 by the Shock Society...
Capture-induced vulnerability in male Shortnose guitarfish during their reproductive period PRADO, ALINE CRISTINA; WOSNICK, NATASCHA; ADAMS, KYE; LEITE, RENATA DALDIN; Carolina Arruda de Oliveira Freire ANIMAL CONSERVATION, v. 2021, p. acv.12734 2021 DOI Palavra-chave: raia viola do focinho curto; capture stress; reprodução Áreas do conhecimento: Ciências Biológicas; Fisiologia da Conservação resumo ...
Carrying eggs in a semi-terrestrial environment: Physiological responses to water deprivation of mothers and embryos of the tree-climbing crab Aratus pisonii MAROCHI, MURILO ZANETTI; CASTELLANO, GIOVANNA CARSTENS; Carolina Arruda de Oliveira Freire; MASUNARI, SETUKO JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY, v. 540, p. 151547 2021 DOI Palavra-chave: caranguejos terrestres; osmorregulação; fêmeas ovígeras Áreas do conhecimento: Ciências Biológicas; Fisiologia Comparada; Fisiologia Comparativa da Osmorregulação resumo ...
Carrying eggs in a semi-terrestrial environment: Physiological responses to water deprivation of mothers and embryos of the tree-climbing crab Aratus pisonii MAROCHI, MURILO ZANETTI; CASTELLANO, GIOVANNA CARSTENS; FREIRE, CAROLINA ARRUDA; Setuko masunari JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY, v. 540, p. 151547-151554, 2021 DOI Palavra-chave: Carbonic anhydrase; osmolality; osmotic stress Áreas do conhecimento: Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia resumo ...
Case 3812 ? Choeridium latum Boucomont, 1928 (currently Ateuchus latus) (Insecta, Coleoptera, Scarabaeidae): proposed conservation of the specifc name CUPELLO, MARIO; Cibele Stramare Ribeiro-Costa; François Génier; Fernando Zagury Vaz-de-Melo Bulletin of Zoological Nomenclature, v. 78, p. 6-16, 2021 Palavra-chave: Nomenclature; Taxonomy Áreas do conhecimento: Ciências Biológicas; Zoologia; Taxonomia dos Grupos Recentes resumo ...
Chaetogastra cogniauxiana (Melastomataceae), a new Brazilian species from the Chaetogastra gracilis complex Fabrício Schmitz Meyer; Renato Goldenberg Phytotaxa, v. 511, p. 261-274, 2021 DOI Áreas do conhecimento: Ciências Biológicas; Taxonomia Vegetal; Taxonomia de Fanerógamos resumo ...

Total de registros: 7170