Publicações - ciências biológicas
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Antonio Ostrensky Neto;
PONT, GIORGI DAL;
LEITE, RAISSA VITÓRIA VIEIRA;
DA SILVA BERTÃO, ANA PAULA;
STICA, PAULA VALESKA;
HORODESKY, ALINE;
MÄDDER NETTO, OTTO SAMUEL;
PIE, MARCIO ROBERTO;
PESTANA, DEBORA;
ZANIN, THIAGO LUIZ
Palavra-chave:
mexilhão-dourado;
qPCR;
Biologia Molecular;
monitoramento ambiental;
Espécies aquáticas invasoras;
eDNA
Áreas do conhecimento:
Ciências Biológicas; Biologia Molecular;
Ciências Biológicas; Ecologia; Ecologia de Ecossistemas; Diagóstico Monitoramento e Recuperação Ambiental de Ambientes Aquáticos Impactados
resumo ...
The invasive mollusc Limnoperna fortunei, commonly known as the golden mussel, poses significant threats to ecosystems and economic sectors, primarily hydroelectric power generation, due to the encrustation of hydraulic structures, which leads to clogging and efficiency loss. Effective monitoring is essential for implementing management strategies against this species. This study compared two methodologies for monitoring L. fortunei: a conventional microscopic inspection, which involves visually identifying and counting the larvae of L. fortunei in water samples, and a molecular approach using environmental DNA (eDNA) via real-time PCR, which detects the presence of L. fortunei by amplifying and analysing its DNA in water samples. Both methods were used to qualitatively and quantitatively assess L. fortunei in ecological water samples across 16 watersheds in Paraná, southern Brazil, aiming to determine their efficacy and complementarity. The molecular approach demonstrated high sensitivity, with results ranging from 50.0 to 97.5% agreement with the conventional method, influenced by environmental and temporal factors. In qualitative comparisons, complete concordance between methods was observed in 72.5% of cases, with molecular tests yielding positive results in 15.5% of cases where conventional methods produced adverse outcomes. Quantitative analysis revealed a weak positive correlation between eDNA concentrations and larval densities (Spearman’s ρ = 0.1878, p = 0.2168), with no significant differences in the distributions of density categories between methods (Wilcoxon test, p = 0.3694) and weak agreement in quantitative analyses (Cohen’s Kappa κ = 0.1509). Each method presented unique strengths: the molecular approach, while highly sensitive, was prone to false negatives due to PCR inhibition, whereas the conventional method faced challenges in detecting low-density populations. The study concludes that combining both approaches provides a more robust monitoring strategy, enhancing detection accuracy across various environmental conditions, and recommends using both methodologies while considering ongoing methodological improvements for effective ecological monitoring of this invasive species.
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CUNHA, REGIANE STAFIM DA;
AZEVEDO, CAROLINA AMARAL BUENO;
MINISKISKOSKY, GUILHERME;
GREGÓRIO, PAULO CÉZAR;
Andréa Emília Marques Stinghen
Palavra-chave:
chronic kidney disease;
Endothelial dysfunction;
Vascular Calcification;
micro-RNAs
Áreas do conhecimento:
Ciências da Saúde; Medicina; Clínica Médica; Nefrologia;
Ciências Biológicas; Bioquímica; Biologia Molecular;
Ciências Biológicas; Biologia Geral; Biologia Celular
resumo ...
Abstract Chronic kidney disease (CKD) is closely associated with endothelial dysfunction and...
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POLIZELI, LEONARDO;
Ângelo Parise Pinto
Palavra-chave:
Blaberinae;
Brachycolini;
Neotropical;
Nomenclature;
Systematics;
Zetoborinae
Áreas do conhecimento:
Ciências Biológicas; Zoologia; Taxonomia dos Grupos Recentes
resumo ...
The blaberids Parahormetica hylaeceps Miranda-Ribeiro, 1936 and Bion mastrucatus Rehn, 1937 were described in the 1930s from single males collected by José Pinto da Fonseca (JPF) in the 1910s. Both specimens came from the region of Mariana municipality, Minas Gerais State, an ecotonal formation between the Atlantic Forest and Cerrado domains in southeastern Brazil. Recently, P. hylaeceps was transferred to Bionoblatta Rehn, 1940, and its strong morphological similarity with Bion mastrucatus (already in Bionoblatta) was highlighted, indicating the need to investigate the validity of these names. Here, we addressed this question by analyzing photos of the type material, comparing original descriptions, and examining data on both the collecting site and the collector’s history. We conclude that Parahormetica hylaeceps should be considered a senior synonym of Bion mastrucatus syn. nov.; hence, the valid name should be Bionoblatta hylaeceps (Miranda-Ribeiro, 1936). We also provide a new diagnosis, synonymy, and review of the distribution of this species, finding Bionoblatta hylaeceps to be endemic to the Itacolomi Peak region. We also present a short account of the work of JPF, including his relevance to Brazilian entomology and itinerary for collecting these specimens. Bionoblatta have five poorly known species of blaberids that, despite recent advances in the genus taxonomy, are still pending review.
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BARTY DEXTRO, RAFAEL;
LOPES FIALHO, LUCIMAR;
DE ARAÚJO NÓBREGA, JOAQUIM;
POLPO, ADRIANO;
Ana Teresa Lombardi
Palavra-chave:
ecofisiologia microalgas;
ecotoxicologia
Áreas do conhecimento:
Ciências Biológicas; ECOTOXICOLOGIA
resumo ...
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RAUTENBERG, KATHLEEN ANGÉLICA;
POLITIKOS, DIMITRIS V.;
Eduardo Luis Cupertino Ballester;
Paulo Ricardo Schwingel;
André Martins Vaz dos Santos
Palavra-chave:
Polyphasic growth;
Huxley model;
Biphasic von Bertalanffy growth model;
purse seine
Áreas do conhecimento:
CIENCIAS_AGRARIAS; Tecnologia de Alimentos;
Ciências Exatas e da Terra; Oceanografia; Oceanografia Biológica; Interação entre os Organismos Marinhos e os Parâmetros Ambientais;
Ciências Biológicas; Zoologia; Taxonomia dos Grupos Recentes
resumo ...
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Leonardo Polizeli Aguiar;
Pinto, Ângelo P.;
Ângelo Parise Pinto
Palavra-chave:
Araucaria Forest;
Blattaria;
Blaberinae;
New Species;
Systematics;
Zetoborinae
Áreas do conhecimento:
Ciências Biológicas; Zoologia; Taxonomia dos Grupos Recentes;
Ciências Biológicas; Zoologia; Taxonomia dos Grupos Recentes
resumo ...
The taxonomically intricate genus of trilobite cockroaches, Parahormetica Brunner von Wattenwyl, 1865, is revised based on a comparative morphological analysis. The goals of this study are to review the nomenclature, propose hypotheses about specific delimitation, and provide diagnoses to allow identification of the taxonomic units in the genus. Based on the revised status of Parahormetica, we transferred Parahormetica hylaeceps Miranda-Ribeiro, 1936, and Parahormetica punctata Saussure, 1873, to the genus Bionoblatta Rehn, 1940. Therefore, the genus includes now four species of giant cockroaches which are predominantly distributed on the Atlantic Forest: Parahormetica bilobata (Saussure, 1864), Parahormetica cicatricosa Saussure, 1869, Parahormetica monticollis (Burmeister, 1838), and Parahormetica museunacional sp. nov. (holotype male deposited in DZUP: Brazil, Paraná). Diagnoses, key, distribution maps, images of living, non-type, and type specimens are made available. Our results make clear that the status and limits among Brachycolini genera pending a full revision.
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RODRIGUES, ANA CAROLINA;
ADAMOSKI, DOUGLAS;
Daniela Fiori Gradia;
DE OLIVEIRA, JAQUELINE CARVALHO
Palavra-chave:
transcribed ultraconserved regions;
cancer;
Biomarkers
Áreas do conhecimento:
Ciências Biológicas; Genética; Genética Animal
resumo ...
Abstract. Ultraconserved regions (UCRs), encompassing 481 genomic segments identical across humans, mice, and rats, and conserved in other species, are distributed across all human chromosomes except 21 and Y. Over 90% of these regions, termed transcribed ultraconserved regions (T-UCRs) are expressed in at least one human tissue. Although extensively studied in the context of cancer since their discovery, the prognostic significance of T-UCRs in a pan-cancer context has not been explored. For this, TCGA data extracted from the TANRIC platform was utilized, differential expression and survival analysis was performed on different tumor types. Our analysis revealed that approximately 300 UCRs were expressed in the majority of the 20 tumor tissues. Additionally, it was noted that all examined tumor tissues expressed a higher number of UCRs compared to their non-tumor counterparts. Through t-distributed stochastic neighbor embedding (t-SNE) analysis, UCR expression patterns could distinguish primary tumor origins and types. Regarding the prognostic value, we identified 100 (20,8%) T-UCRs associated with disease-specific survival (DSS) and 102 (21.2%) T-UCRs associated with progression-free interval (PFI). Those T-UCRs are particularly important in the survival outcomes of kidney renal clear cell carcinoma (KIRC) and low-grade gliomas (LGC). In addition, we verified that uc.44, uc.48, uc.135, uc.144, uc.153, uc.217, uc.255, uc.256, uc.344, uc.357, uc.390, uc.427 and uc.436 were associated with survival of more than one tumor. Particularly, uc.135 high expression was associated with poor prognosis in Kidney Renal Papillary Cell Carcinoma (KIRP) and LGC, and good prognosis in KIRC. Uc.135 are mapped on MECOM gene, coding an oncoprotein acting as transcriptional regulator and involved in apoptosis, proliferation, and cell differentiation. Our findings suggest that analysis of T-UCRs expression have the potential to be used as predictive biomarkers and can help in studying the mechanisms and specific roles of the highlighted T-UCRs in cancer.Citation Format: Ana Carolina Rodrigues, Douglas Adamoski, Daniela F. Gradia, Jaqueline Carvalho de Oliveira. Transcribed ultraconserved regions are associated with survival in multiple tumor types [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 3681.
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DE SOUZA VALENTE, CECÍLIA;
MENDES, ARIELLY FÁVARO;
DO NASCIMENTO FERREIRA, CAIO HENRIQUE;
BALDISSEROTTO, BERNARDO;
HEINZMANN, BERTA MARIA;
André Martins Vaz dos Santos;
Eduardo Luis Cupertino Ballester
Palavra-chave:
Aquaculture;
Crustacean welfare;
decapod anaesthesia;
giant river prawn;
Plant-derived anaesthetic
Áreas do conhecimento:
CIENCIAS_AGRARIAS; Tecnologia de Alimentos;
Ciências Biológicas; Zoologia; Zoologia Aplicada; Conservação das Espécies Animais
resumo ...
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DE SOUZA VALENTE, CECÍLIA;
Eduardo Luis Cupertino Ballester;
COATES, CHRISTOPHER J.;
CAGOL, LUANA;
BOMBARDELLI, ROBIE ALLAN;
BECKER, ALEXSSANDRO GEFERSON;
SCHMIDT, DENISE;
HEINZMANN, BERTA MARIA;
André Martins Vaz dos Santos;
BALDISSEROTTO, BERNARDO
Palavra-chave:
antioxidant enzymes;
Feed supplement;
Lemon verbena;
Crustacean welfare;
Innate immunity
Áreas do conhecimento:
CIENCIAS_AGRARIAS; Aqüicultura;
Ciências Biológicas; Zoologia; Zoologia Aplicada; Conservação das Espécies Animais
resumo ...
Incorporating essential oils as dietary supplements in aquaculture is increasing in popularity. In prawn farming, their use is associated with reduced disease outbreaks, enhanced immunity, and improved animal growth and welfare. Herein, we explored the use of lemon beebrush essential oil (Aloysia triphylla; EOAT) in the diet of juvenile giant freshwater prawns (Macrobrachium rosenbergii) and its potential impact on growth performance and oxidative status of the hepatopancreas. Four hundred juvenile M. rosenbergii (0.40 ± 0.19 g, similar weights between sexes) were fed diets containing concentrations of EOAT at 0.0% (control), 0.1%, 0.2%, or 0.3% for 50 days. Each experimental group had five replicates, with 20 prawns per experimental unit. Prawn survival remained above 84% for all experimental groups. Prawn weight gain, specific growth rate, feed conversion ratio, and body condition were similar among all experimental groups. Dietary EOAT increased activities of the enzymatic antioxidants, catalase and superoxide dismutase, and varied glutathione S transferase activity in the hepatopancreas of prawns when compared to those fed the control diet. Dietary EOAT did not affect malondialdehyde (lipid peroxidation) levels. While EOAT had no impact on survival and growth of M. rosenbergii, it may modulate the detoxification capacity of the hepatopancreas (i.e., metabolic resistance). Future work should prospect EOAT as part of a nutraceutical blend, especially when combined with known growth enhancers and immune stimulants. Based on our results, dietary inclusion of 0.2% EOAT is the most suitable for juvenile M. rosenbergii farming.
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AUER, EDUARDO DELABIO;
BUMILLER-BINI HOCH, VALÉRIA;
DA SILVA, EMILIANO BORGES;
ZONTA, YOHAN RICCI;
DIAS-MELICIO, LUCIANE ALARCÃO;
LAROCCA SKARE, THELMA;
PICCELI, VANESSA F.;
MESSIAS-REASON, IARA;
Angelica Beate Winter Boldt
Palavra-chave:
lupus;
NET;
NETose;
Raynaud;
neutrófilos;
autoimunidade
Áreas do conhecimento:
Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia
resumo ...
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