Publicações - ciências biológicas
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Carolina Amaral Bueno Azevedo;
Regiane Stafim da Cunha;
Carolina Victoria Cruz Junho;
Jessica Verônica da Silva;
Andréa Moreno-Amaral;
Thyago Proença de Moraes;
CARNEIRO-RAMOS, MARCELA SORELLI;
Andréa Emília Marques Stinghen
Palavra-chave:
Uremic Toxins;
peritoneal dialysis;
extracellular vesicles
Áreas do conhecimento:
Ciências da Saúde; nephrology;
Ciências da Saúde; Medicina; Nefrologia Experimental;
Ciências Biológicas; Bioquímica; Bioquímica
resumo ...
Uremic toxins (UTs) accumulate in the circulation of patients with chronic kidney disease (CKD). High volume hemodiafiltration (HDF) improves clearanc…
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BERTI, FERNANDA COSTA BRANDÃO;
MATHIAS, CAROLINA;
GARCIA, LEANDRO E.;
Daniela Fiori Gradia;
DE ARAÚJO SOUZA, PATRÍCIA SAVIO;
CIPOLLA, GABRIEL ADELMAN;
DE OLIVEIRA, JAQUELINE CARVALHO;
MALHEIROS, DANIELLE
Palavra-chave:
competing endogenous;
Xist;
HPV16;
HPV18;
cervical cancer
Áreas do conhecimento:
Ciências Biológicas; Genética Humana e Médica; Genética Geral;
Ciências Biológicas; Genética; Genética Humana e Médica; Imunogenética
resumo ...
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Fernanda Kashiwagi;
Brenno Wendler Miranda;
Fábio de Oliveira Pedrosa;
Emanuel Maltempi de Souza;
Marcelo Muller dos Santos
Palavra-chave:
Interação Planta-Microrganismo;
Biologia Sintética
Áreas do conhecimento:
Ciências Biológicas; Biologia Sintética
resumo ...
Control of gene expression is crucial for several biotechnological applications, especially for implementing predictable and controllable genetic circuits. Such circuits are often implemented with a transcriptional regulator activated by a specific signal. These regulators should work independently of the host machinery, with low gratuitous induction or crosstalk with host components. Moreover, the signal should also be orthogonal, recognized only by the regulator with minimal interference with the host operation. In this context, transcriptional regulators activated by plant metabolites as flavonoids emerge as candidates to control gene expression in bacteria. However, engineering novel circuits requires the characterization of the genetic parts (e.g., genes, promoters, ribosome binding sites, and terminators) in the host of interest. Therefore, we decomposed the QdoR regulatory system of B. subtilis, responsive to the flavonoid quercetin, and reassembled its parts into genetic circuits programmed to have different levels of gene expression and noise dependent on the concentration of quercetin. We showed that only one of the promoters regulated by QdoR worked well in E. coli, enabling the construction of other circuits induced by quercetin. The QdoR expression was modulated with constitutive promoters of different transcriptional strengths, leading to low expression levels when QdoR was highly expressed and vice versa. E. coli strains expressing high and low levels of Qdo...
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KOCH, GABRIEL;
LUCIMERIS RUARO SCHUTA;
CALEGARIO, RENATA FAIER;
João Carlos Bespalhok Filho;
DAROS, EDELCLAITON;
Ricardo Augusto de Oliveira;
DUARTE, HENRIQUE DA SILVA SILVEIRA
Palavra-chave:
Sugarcane breeding;
ferrugem;
seleção;
ferrugem alaranjada;
ferrugem marrom
Áreas do conhecimento:
Ciências Biológicas; Biotecnologia; Biotecnologia Vegetal; Melhoramento de Plantas
resumo ...
Orange rust (OR) and brown rust (BR) are among the most important diseases of sugarcane. The objective of this work was to evaluate the efficiency of fungicides in the control of both rusts. Two experiments, one for each rust, were conducted in the state of Paraná, Brazil, in plant cane and in ratoon. A randomized block design with 8 treatments and three replications was used. The treatments were: control (T1), pyraclostrobin (T2), pyraclostrobin + epoxiconazole (T3), azoxystrobin + cyproconazole (T4), azoxystrobin + tebuconazole (T5), trifloxystrobin + tebuconazole (T6), azoxystrobin + benzovindiflupyr (T7) and pyraclostrobin + fluxapyroxad (T8). The cultivars RB72454 and RB835486 were used for their susceptibility to OR and BR, respectively. Four applications of fungicides were carried out in plant cane and also in ratoon, in a 30-day intervals. With the severity data over time, the relative area under the disease progress curve (rAUDPC) was calculated. After harvest, yield (tons of cane per hectare-TCH), yield recovery compared to the control (YR) (%), pol% cane (PC) and tons of pol per hectare (TPH) were obtained. From rAUDPC results, treatments T3, T4, T5, and T7 were the most efficient for the control of OR and treatments T2, T3, T4, T5, T7 and T8 were the most efficient for controlling BR. Yield was affected by treatments only in ratoon in both experiments. The YR ranged from 18.5% to 31.1% and from 16.1% to 37.7% to OR and BR, respectively. There were no differences between treatments for PC in both experiments and TPH was influenced by treatments in ratoon in the BR experiment.
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KOCH, GABRIEL;
LUCIMERIS RUARO SCHUTA;
CALEGARIO, RENATA FAIER;
João Carlos Bespalhok Filho;
DAROS, EDELCLAITON;
Ricardo Augusto de Oliveira;
DUARTE, HENRIQUE DA SILVA SILVEIRA
Palavra-chave:
Sugarcane breeding;
ferrugem;
seleção;
ferrugem alaranjada;
ferrugem marrom
Áreas do conhecimento:
Ciências Biológicas; Biotecnologia; Biotecnologia Vegetal; Melhoramento de Plantas
resumo ...
Orange rust (OR) and brown rust (BR) are among the most important diseases of sugarcane. The objective of this work was to evaluate the efficiency of fungicides in the control of both rusts. Two experiments, one for each rust, were conducted in the state of Paraná, Brazil, in plant cane and in ratoon. A randomized block design with 8 treatments and three replications was used. The treatments were: control (T1), pyraclostrobin (T2), pyraclostrobin + epoxiconazole (T3), azoxystrobin + cyproconazole (T4), azoxystrobin + tebuconazole (T5), trifloxystrobin + tebuconazole (T6), azoxystrobin + benzovindiflupyr (T7) and pyraclostrobin + fluxapyroxad (T8). The cultivars RB72454 and RB835486 were used for their susceptibility to OR and BR, respectively. Four applications of fungicides were carried out in plant cane and also in ratoon, in a 30-day intervals. With the severity data over time, the relative area under the disease progress curve (rAUDPC) was calculated. After harvest, yield (tons of cane per hectare-TCH), yield recovery compared to the control (YR) (%), pol% cane (PC) and tons of pol per hectare (TPH) were obtained. From rAUDPC results, treatments T3, T4, T5, and T7 were the most efficient for the control of OR and treatments T2, T3, T4, T5, T7 and T8 were the most efficient for controlling BR. Yield was affected by treatments only in ratoon in both experiments. The YR ranged from 18.5% to 31.1% and from 16.1% to 37.7% to OR and BR, respectively. There were no differences between treatments for PC in both experiments and TPH was influenced by treatments in ratoon in the BR experiment.
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C A Oliveira Ribeiro;
Azevedo, Juliana S.;
Robie Alan Bombardelli;
Marco A F Randi
Palavra-chave:
MXR;
Rhamdia quelen;
Cobre Inorganico;
biomarcadores;
Estagio Inicial de desenvolvimento
Áreas do conhecimento:
Ciências Biológicas; Ecologia Aplicada; Ecotoxicologia
resumo ...
(en)
P-glycoproteins (P-gp) and Multidrug resistance protein (MRP) represent a family of ABC (ATP-binding cassette) transporters responsible for multixenobiotic resistance mechanism (MXR) in aquatic organisms. In the current study the modulation of P-gp and MRP proteins was evaluated in embryo and larvae of Rhamdia quelen fish species exposed to copper. Adult females were exposed by gavage during 60 days to copper (5 mg Cu kg-1) and eggs, embryos, and larvae from exposed and unexposed females were exposed to 30 mg Cu L-1. The activity of ABC transporters was accessed via calcein accumulation assay using the specific inhibitors: Verapamil (P-gp) and MK571 (MRP). P-gp activity was detected in all analyzed stages whereas MRP activity was observed after 36 and 96 hpf. Oocytes from females previously exposed and larvae stages (36 and 96 hpf) accumulated less calcein than no exposed oocytes, showing higher ABC transporters activity. In individuals exposed to copper, a higher inhibitory effect was observed 1 hpf. The modulation of ABC transporter proteins is time dependent throughout the development, and the initial stages are more sensible to copper. These findings highlight the MXR mechanism as a biomarker of pollutant exposure in early stages of development of R. quelen.
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C.A., OLIVEIRA RIBEIRO;
A.C.B., AZEVEDO;
R.A., BOMBARDELLI;
M. A. F. Randi
Palavra-chave:
mecanismo MXR;
Rhamdia quelem;
biomarcadores;
Cobre Inorganico;
estágios iniciais do desenvolvimento
Áreas do conhecimento:
Ciências Biológicas; Ecologia; Ecologia Aplicada
resumo ...
(en)
P-glycoproteins (P-gp) and Multidrug resistance protein (MRP) represent a family of ABC (ATP-binding cassette) transporters responsible for multixenobiotic resistance mechanism (MXR) in aquatic organisms. In the current study the modulation of P-gp and MRP proteins was evaluated in embryo and larvae of Rhamdia quelen fish species exposed to copper. Adult females were exposed by gavage during 60 days to copper (5 mg Cu kg-1) and eggs, embryos, and larvae from exposed and unexposed females were exposed to 30 mg Cu L-1. The activity of ABC transporters was accessed via calcein accumulation assay using the specific inhibitors: Verapamil (P-gp) and MK571 (MRP). P-gp activity was detected in all analyzed stages whereas MRP activity was observed after 36 and 96 hpf. Oocytes from females previously exposed and larvae stages (36 and 96 hpf) accumulated less calcein than no exposed oocytes, showing higher ABC transporters activity. In individuals exposed to copper, a higher inhibitory effect was observed 1 hpf. The modulation of ABC transporter proteins is time dependent throughout the development, and the initial stages are more sensible to copper. These findings highlight the MXR mechanism as a biomarker of pollutant exposure in early stages of development of R. quelen.
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David Livingstone Alves Figueiredo;
Anelisa Ramão;
Angelica Beate Winter Boldt;
Carla Fredrichsen Moya;
Chung Man Chin;
Daniel de Paula;
Daniel Rech;
Daniela Fiori Gradia;
Danielle Malheiros;
Danielle Venturini;
Eliandro Reis Tavares;
João Paulo Bianchi Ximenez;
Emerson Carraro;
Fábio Rodrigues Ferreira Seiva;
Carolina Panis;
Rafael dos Santos Bezerra;
Adriano Ferrasa;
Alessandra Lourenço Cecchini;
Alexandra Ivo de Medeiros;
Ana Marisa Fusco Almeida
Palavra-chave:
COVID-19;
SARS-CoV-2;
resposta imune;
vacina;
genética epidemiológica
Áreas do conhecimento:
Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia;
Ciências Biológicas; Genética; Genética Humana e Médica; Imunogenética
resumo ...
Abstract Coronavirus disease 2019 (COVID-19), caused by the Severe Acute Respiratory Syndrome...
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FIGUEIREDO, DAVID LIVINGSTONE ALVES;
RAMÃO, ANELISA;
Boldt, Angelica Beate Winter;
MOYA, CARLA FREDRICHSEN;
CHIN, CHUNG MAN;
PAULA, DANIEL DE;
RECH, DANIEL;
Daniela Fiori Gradia;
MALHEIROS, DANIELLE;
VENTURINI, DANIELLE;
TAVARES, ELIANDRO REIS;
XIMENEZ, JOÃO PAULO BIANCHI;
CARRARO, EMERSON;
SEIVA, FÁBIO RODRIGUES FERREIRA;
PANIS, CAROLINA;
BEZERRA, RAFAEL DOS SANTOS;
FERRASA, ADRIANO;
CECCHINI, ALESSANDRA LOURENÇO;
MEDEIROS, ALEXANDRA IVO DE;
ALMEIDA, ANA MARISA FUSCO
Palavra-chave:
COVID-19;
therapeutic interventions;
global health treat;
virus diversity
Áreas do conhecimento:
Ciências da Saúde; Farmacologia; Transmissão Neuromuscular;
Ciências Biológicas; Genética; Genética Humana e Médica; Imunogenética
resumo ...
Abstract Coronavirus disease 2019 (COVID-19), caused by the Severe Acute Respiratory Syndrome...
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Hanna Karolyna dos Santos;
Patrícia da Silva Dias;
Rafael Ernesto Balen;
Izabel Volkweis Zadinelo;
Robie Allan Bombardelli;
Fábio Meurer
Palavra-chave:
bagre;
desempenho;
peixes nativos;
piscicultura;
zoologia aplicada
Áreas do conhecimento:
CIENCIAS_AGRARIAS; Aqüicultura;
CIENCIAS_AGRARIAS; Zootecnia; Aquicultura; Piscicultura;
Ciências Biológicas; Zoologia; Zoologia Aplicada; Conservação das Espécies Animais
resumo ...
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