Publicações - ciências biológicas
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Paulo de Tarso Cunha Chaves
Palavra-chave:
estuários;
Fishing gear;
Captura incidental;
Pesca-fantasma;
Bycatch
resumo ...
Abstract Comparisons between the implications of commercial and recreational fisheries for...
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Zelinda SCHEMCZSSEN;
Fernando Hitomi Matsubara;
Andrea Senff Ribeiro;
Luiza Helena Gremski;
Silvio Sanches Veiga;
Hanna Camara Justa;
Jenifer Nowatzki;
Baldissera, A.B.;
Nayanne Louise Costacurta Polli;
Elidiana de Bona;
Izadora Volpato Rossi;
Marcel Ivan Ramirez;
João Minozzo
Palavra-chave:
Aranha marrom;
Veneno;
serpina;
Propriedades biológicas;
Propriedades funcionais
Áreas do conhecimento:
Ciências Biológicas; Morfologia; Citologia e Biologia Celular;
Ciências Biológicas; Bioquímica; Biologia Molecular
resumo ...
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DE SOUZA, IRISDORIS RODRIGUES;
LEME, DANIELA MORAIS;
CANAVEZ, ANDREZZA DI PIETRO MICALI;
SCHUCK, DESIREE CIGARAN;
GAGOSIAN, VIVIANA STEPHANIE COSTA;
DE SOUZA, ISISDORIS RODRIGUES;
Taynah Vicari;
DA SILVA TRINDADE, EDVALDO;
Cestari, M.M.;
LORENCINI, MÁRCIO
Palavra-chave:
ensaio cometa;
ZFL;
peixe
Áreas do conhecimento:
Ciências Biológicas; Genética; Genética Animal; Mutagênese Ambiental;
Ciências Biológicas; Genética; genotoxicidade
resumo ...
Fish cell spheroids are promising 3D culture models for vertebrate replacement in ecotoxicology. However, new alternative ecotoxicological methods must be adapted for applications in industry and for regulatory purposes; such methods must be cost-effective, simple to manipulate and provide rapid results. Therefore, we compared the effectiveness of the traditional hanging drop (HD), orbital shaking (OS), and HD combined with OS (HD+OS) methods on the formation of zebrafish cell line spheroids (ZFL and ZEM2S). Time in HD (3–5 days) and different 96-well plates [flat-bottom or ultra-low attachment of round-bottom (ULA-plates)] in OS were evaluated. Easy handling, rapid spheroid formation, uniform-sized spheroids, and circularity were assessed to identify the best spheroid protocol. Traditional HD alone did not result in ZFL spheroid formation, whereas HD (5 days)+OS did. When using the OS, spheroids only formed on the ULA-plate. Both HD+OS and OS were reproducible in size (177.50 ± 2.81 µm and 225.62 ± 19.20 µm, respectively) and circularity (0.83 ± 0.02 and 0.80 ± 0.01, respectively) of ZFL spheroids. Nevertheless, HD+OS required a considerable time to completely form spheroids (10 days) and intensive handling, whereas the OS was fast (5 days of incubation) and simple. OS also yielded reproducible ZEM2S spheroids in 1 day (226.23 ± 0.57 µm diameter and 0.80 ± 0.01 circularity). In conclusion, OS in ULA-plate is an effective and simple spheroid protocol for high-throughput ecotoxicity testing. This study contributes to identify a fast, reproducible, and simple protocol of single piscine spheroid formation in 96-well plates and supports the application of fish 3D model in industry and academia.
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MANTOAN, PAULO;
Eduardo Luis Cupertino Ballester;
RAMAGLIA, ANDRESSA CRISTINA;
AUGUSTO, ALESSANDRA
Palavra-chave:
prawn culture
Áreas do conhecimento:
CIENCIAS_AGRARIAS; Aqüicultura;
Ciências Biológicas; Biologia Geral; Aquacultura; Carcinicultura
resumo ...
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Tamyres Mingorance Carvalho;
Renata Montoro Dourado;
Sueli Massumi Nakatani;
Cesar Augusto Barros Duarte;
Felipe Tuon;
Sergio Ossamu Ioshii;
Irina Nastassja Riediger;
Angelica Beate Winter Boldt
Palavra-chave:
dPCR;
PCR digital;
EGFR;
Câncer de Pulmao;
FFPE;
cancer
Áreas do conhecimento:
Ciências Biológicas; Oncogenética;
Ciências Biológicas; Genética; Genética Humana e Médica; Imunogenética
resumo ...
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DE ALMEIDA, MARCUS;
Ângelo Parise Pinto;
CARVALHO, ALCIMAR
Palavra-chave:
odonates;
biological data;
zoological collections;
Taxonomy;
Arthropoda;
invertebrates
Áreas do conhecimento:
Ciências Biológicas; Zoologia; Taxonomia dos Grupos Recentes
resumo ...
Natural history collections (NHC) are guardians of biodiversity (Lane 1996) and essential to understand the natural world and its evolutionary processes. They hold samples of morphological and genetic heritages of living and extinct biotas, helping to reconstruct the timeline of life over the centuries (Gardner 2014). Primary data from specimens in NHC are crucial elements for research in many areas of biological sciences, considered the “bricks” of systematics and therefore one of the pillars for evolutionary studies (Troudet 2018). For this reason, studies carried out in NHC are essential for the development of the scientific knowledge and are pivotal for the scientific-technological progress of a nation (Camargo 2015).The digitization and availability of primary data on biodiversity from NHC represents a inexpensive, practical and secure means of exchanging information, allowing collaboration between institutions and researchers. In this sense, initiatives such as the Sistema de Informação sobre a Biodiversidade Brasileira (SiBBr), a country-level branch of the Global Biodiversity Information Facility (GBIF) platform, aim to encourage and establish ways for the informatization of biological collections and their type specimens.Known for housing one of the largest and oldest collections of insects in the world focused on Neotropical fauna, the Entomological Collection of the Museu Nacional of Federal University of Rio de Janeiro (MNRJ) had more than 3,000 primary types and approximately 12,005,000 specimens, of which about 96% were lost in the tragic fire occurred at the institution on September 2, 2018. The SiBBr project was active in that collection from 2016 to 2019 and enabled the digitization and preservation of data from the type material of many insect orders, including the charismatic dragonflies (order Odonata). Due to the end of the agreement between SiBBr and the Museu Nacional, most of the obtained primary data are pending full curation and, therefore, are not yet available to the public and researchers.The MNRJ housed the biggest and most important collection of dragonflies among all Central and South American institutions. It assembled most of the physical records of neotropical dragonfly fauna gathered over the last 80 years, many of which are of undescribed taxa. Unfortunately, almost all material was permanently lost. This study aims to gather, analyze and publicize primary data of the type material of dragonflies housed in the MNRJ, ensuring the preservation of its history, as well as providing data on the taxonomy and diversity of this marvelous group of insects.A total of 11 families, 50 genera and 131 species were recorded, belonging to the suborders Anisoptera and Zygoptera with distributional records widespread in South America.The MNRJ housed 105 holotypes of dragonflies' nomina representing 11.7% of the richness of the Brazilian Odonata fauna (901 spp.), a country with the highest number of species of the biosphere. The impact of the loss of this collection to studies of these insects is unprecedented, since some enigmatic and monotypic genera such as Brasiliogomphus, Fluminagrion and Roppaneura lost 100% of their type series, while others most diverse such as Lauromacromia, Oxyagrion and Neocordulia lost 50%, 35% and 31% of their holotypes. Therefore, due to the registration and preservation of primary biodiversity data, this work reiterates the importance of curating and digitizing biological scientific collections. Furthermore, it shows extreme relevance for preserving information on existing biodiversity permanently and providing support for future research. Digitization and interconnecting digital extended specimen data proves to be one of the main and most effective ways to protect NHC heritage and their primary data against catastrophic events.
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CERON, F. JULIANE M.;
Viviane Prodocimo;
Carolina Arruda de Oliveira Freire
Palavra-chave:
fish;
gills;
NaK-ATPase
Áreas do conhecimento:
Ciências Biológicas; Fisiologia Comparada; Fisiologia Comparativa da Osmorregulação
resumo ...
Ray-finned fishes of the superorder Ostariophysi are primarily freshwater (FW), and normally stenohaline. Differently, fishes of the superorder Acanthopterygii are essentially marine, and frequently euryhaline, with some secondary FW. Na+/K+-ATPase-immunoreactive ionocytes were localized in the branchial epithelia of 4 species of Ostariophysi and 3 of Acanthopterygii. The Ostariophysi grass carp (Ctenopharyngodon idella, Cypriniformes), twospot Astyanax (Astyanax bimaculatus) and piracanjuba (Brycon orbignyanus), Characiformes, and the jundiá (Rhamdia quelen, Siluriformes), all from FW, displayed ionocytes in the filament plus secondary lamellae (F + SL). In their turn, all the three species of Acanthopterygii showed immunoreactive ionocytes in the filaments only (F). They were the Nile tilapia (Oreochromis niloticus, Cichliformes) in FW, the dog snapper (Lutjanus jocu, Perciformes) in seawater (SW), and the green puffer (Sphoeroides greeleyi, Tetraodontiformes) in SW. Ionocytes normally extend their distribution to the secondary lamellae (F + SL) in Ostariophysi. In Acanthopterygii, we find more plasticity: ionocytes are more frequently restricted to the filament in SW, but also spread to SL in FW. It may be that the occurrence of ionocytes in SL is the ancestral condition, but some euryhaline acanthopterygians rely on the space of the SL for placement of additional ionocytes when in FW absorbing salt. Our study contributed to the identification of the pattern of ionocyte distribution in gills of Ostariophysi in respect to that of Acanthopterygii.
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JAQUELINE PRISCILA BISPO DE ALMEIDA CARDOSO MARCONDES;
Rafael Trevisan;
Maria Cecilia de Chiara Moço;
Cleusa Bona
Palavra-chave:
Eleocharis;
Anatomia do escapo;
aerenchyma;
anatomy of aquatic plant;
Evolução do aerênquima
Áreas do conhecimento:
Ciências Biológicas; Botânica; Morfologia Vegetal; Anatomia Vegetal
resumo ...
Abstract Eleocharis (Cyperaceae) includes more than 300 species of perennial or annual herbs,...
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GROSSEL, LAÍS ALINE;
JAVOROUSKI, MANOEL LUCAS;
Nei Moreira;
DE ARAUJO MONTEIRO-FILHO, EMYGDIO LEITE
Palavra-chave:
queixada;
comportamento animal;
Dominância;
hierarquia;
Tayassu pecari;
Taiassuídeo
Áreas do conhecimento:
CIENCIAS_AGRARIAS; Fisiologia Veterinária; Endocrinologia;
CIENCIAS_AGRARIAS; Medicina Veterinária; Fisiologia Veterinária; Fisiologia da Reprodução;
Ciências Biológicas; Zoologia; Comportamento Animal
resumo ...
Dominance hierarchies are typically stable, with dominants occupying central positions in social interaction networks. However, system perturbations, such as the removal of individuals, may cause instability, which varies according to the group’s resilience. If the hierarchy undergoes a restructuring, this can occur through a dynamic process of self-organization (Social Dynamics hypothesis) or through the influence of individuals’ attributes (Previous Attributes hypothesis). We analyzed the resilience of the white-lipped peccary hierarchy after the alpha’s death and observed how the rise of a new dominant occurred. Additionally, we evaluated the validity of these two hypotheses in the restructuring of the system. We observed the group of white-lipped peccary males of the Municipal Zoo of Curitiba, PR, Brazil, from May to October 2018. We recorded and analyzed the agonistic and affiliative interactions, and we collected data from the attributes: weight, testicle size, testosterone serum and age, before and after the dominant’s death (August). Due to this perturbation, the hierarchy started to show instability, but proved to be resilient. There was an increase in agonism and more than one individual pleading for the new dominant position, which was occupied by a subordinate male. Affiliative interactions were also important in the rise of the new alpha. The hierarchy was well ordered by the social dynamics among individuals, but weight and testicular volume were also correlated with the status of the individuals before, and with the serum testosterone after the alpha’s death. Thus, both hypotheses affected the restructuring of this system.
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GROSSEL, LAÍS ALINE;
JAVOROUSKI, MANOEL LUCAS;
MOREIRA, NEI;
Emygdio Leite de Araújo Monteiro-Filho
Palavra-chave:
Previous attributes;
Self-organization;
Social interactions;
Social Network Analysis;
Tayassu pecari;
Tayassuidae
Áreas do conhecimento:
Ciências Biológicas; Zoologia; Etologia;
Ciências Biológicas; Zoologia; Comportamento Animal
resumo ...
Dominance hierarchies are typically stable, with dominants occupying central positions in social interaction networks. However, system perturbations, such as the removal of individuals, may cause instability, which varies according to the group’s resilience. If the hierarchy undergoes a restructuring, this can occur through a dynamic process of self-organization (Social Dynamics hypothesis) or through the influence of individuals’ attributes (Previous Attributes hypothesis). We analyzed the resilience of the white-lipped peccary hierarchy after the alpha’s death and observed how the rise of a new dominant occurred. Additionally, we evaluated the validity of these two hypotheses in the restructuring of the system. We observed the group of white-lipped peccary males of the Municipal Zoo of Curitiba, PR, Brazil, from May to October 2018. We recorded and analyzed the agonistic and affiliative interactions, and we collected data from the attributes: weight, testicle size, testosterone serum and age, before and after the dominant’s death (August). Due to this perturbation, the hierarchy started to show instability, but proved to be resilient. There was an increase in agonism and more than one individual pleading for the new dominant position, which was occupied by a subordinate male. Affiliative interactions were also important in the rise of the new alpha. The hierarchy was well ordered by the social dynamics among individuals, but weight and testicular volume were also correlated with the status of the individuals before, and with the serum testosterone after the alpha’s death. Thus, both hypotheses affected the restructuring of this system.
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