Publicações - ciências biológicas
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OLIVEIRA, LUCIANA KELLY;
PILZ, LETÍCIA;
FURTADO, PLÍNIO SCHMIDT;
Eduardo Luis Cupertino Ballester;
DE ALMEIDA BICUDO, ÁLVARO JOSÉ
Palavra-chave:
tilápias;
bft;
nutrition
Áreas do conhecimento:
CIENCIAS_AGRARIAS; Aqüicultura;
Ciências Biológicas; Biologia Geral; Piscicultura
resumo ...
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KRETZSCHMAR, GABRIELA CANALLI;
ALENCAR, NINA MOURA;
DA SILVA, SARITHA SUELLEN LOPES;
SULZBACH, CARLA DANIELA;
MEISSNER, CAROLINE GRISBACH;
Maria Luiza Petzl-Erler;
SOUZA, RICARDO LEHTONEN R.;
Boldt, Angelica Beate Winter
Palavra-chave:
LOAD;
Alzheimer Disease;
disease susceptibility;
ncRNA;
genetic association
Áreas do conhecimento:
Ciências Biológicas; Genética Humana e Médica;
Ciências Biológicas; Bioquímica; Biologia Molecular;
Ciências Biológicas; Imunologia; Imunogenética
resumo ...
Several genome-wide association studies (GWAS) have been carried out with late-onset Alzheimer’s disease (LOAD), mainly in European and Asian populations. Different polymorphisms were associated, but several of them without a functional explanation. GWAS are fundamental for identifying loci associated with diseases, although they often do not point to causal polymorphisms. In this sense, functional investigations are a fundamental tool for discovering causality, although the failure of this validation does not necessarily indicate a non-causality. Furthermore, the allele frequency of associated genetic variants may vary widely between populations, requiring replication of these associations in other ethnicities. In this sense, our study sought to replicate in 150 AD patients and 114 elderly controls from the South Brazilian population 18 single-nucleotide polymorphisms (SNPs) associated with AD in European GWAS, with further functional investigation using bioinformatic tools for the associated SNPs. Of the 18 SNPs investigated, only four were associated in our population: rs769449 (APOE), rs10838725 (CELF1), rs6733839, and rs744373 (BIN1–CYP27C1). We identified 54 variants in linkage disequilibrium (LD) with the associated SNPs, most of which act as expression or splicing quantitative trait loci (eQTLs/sQTLs) in genes previously associated with AD or with a possible functional role in the disease, such as CELF1, MADD, MYBPC3, NR1H3, NUP160, SPI1, and TOMM40. Interestingly,...
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Gabriela Canalli Kretzschmar;
Nina de Moura Alencar;
Saritha Suelen Lopes da Silva;
Carla Daniela Sulzbach;
Caroline Grisbach Meissner;
Maria Luiza Petzl-Erler;
Ricardo Lehtonen Rodrigues de Souza;
Angelica Beate Winter Boldt
Palavra-chave:
doença de Alzheimer;
lncRNA;
GWAS;
APOE;
BIN1;
CR1
Áreas do conhecimento:
Ciências Biológicas; Genética Humana e Médica;
Ciências Biológicas; Genética; Genética Humana e Médica; Imunogenética
resumo ...
Several genome-wide association studies (GWAS) have been carried out with late-onset Alzheimer’s disease (LOAD), mainly in European and Asian populations. Different polymorphisms were associated, but several of them without a functional explanation. GWAS are fundamental for identifying loci associated with diseases, although they often do not point to causal polymorphisms. In this sense, functional investigations are a fundamental tool for discovering causality, although the failure of this validation does not necessarily indicate a non-causality. Furthermore, the allele frequency of associated genetic variants may vary widely between populations, requiring replication of these associations in other ethnicities. In this sense, our study sought to replicate in 150 AD patients and 114 elderly controls from the South Brazilian population 18 single-nucleotide polymorphisms (SNPs) associated with AD in European GWAS, with further functional investigation using bioinformatic tools for the associated SNPs. Of the 18 SNPs investigated, only four were associated in our population: rs769449 (APOE), rs10838725 (CELF1), rs6733839, and rs744373 (BIN1–CYP27C1). We identified 54 variants in linkage disequilibrium (LD) with the associated SNPs, most of which act as expression or splicing quantitative trait loci (eQTLs/sQTLs) in genes previously associated with AD or with a possible functional role in the disease, such as CELF1, MADD, MYBPC3, NR1H3, NUP160, SPI1, and TOMM40. Interestingly,...
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JUNHO, CAROLINA VICTÓRIA CRUZ;
AZEVEDO, CAROLINA AMARAL BUENO;
DA CUNHA, REGIANE STAFIM;
DE YURRE, AINHOA RODRIGUEZ;
MEDEI, EMILIANO;
Andréa Emília Marques Stinghen;
CARNEIRO-RAMOS, MARCELA SORELLI
Palavra-chave:
Heat shock proteins;
heart;
Kidney;
Cardiorenal syndrome
Áreas do conhecimento:
Ciências da Saúde; Medicina; Clínica Médica; Nefrologia;
Ciências Biológicas; Fisiologia; Fisiologia de Órgãos e Sistemas; Fisiologia Endócrina;
Ciências Biológicas; Bioquímica; Bioquímica
resumo ...
Over the development of eukaryotic cells, intrinsic mechanisms have been developed in order to provide the ability to defend against aggressive agents. In this sense, a group of proteins plays a crucial role in controlling the production of several proteins, guaranteeing cell survival. The heat shock proteins (HSPs), are a family of proteins that have been linked to different cellular functions, being activated under conditions of cellular stress, not only imposed by thermal variation but also toxins, radiation, infectious agents, hypoxia, etc. Regarding pathological situations as seen in cardiorenal syndrome (CRS), HSPs have been shown to be important mediators involved in the control of gene transcription and intracellular signaling, in addition to be an important connector with the immune system. CRS is classified as acute or chronic and according to the first organ to suffer the injury, which can be the heart (CRS type 1 and type 2), kidneys (CRS type 3 and 4) or both (CRS type 5). In all types of CRS, the immune system, redox balance, mitochondrial dysfunction, and tissue remodeling have been the subject of numerous studies in the literature in order to elucidate mechanisms and propose new therapeutic strategies. In this sense, HSPs have been targeted by researchers as important connectors between kidney and heart. Thus, the present review has a focus to present the state of the art regarding the role of HSPs in the pathophysiology of cardiac and renal alterations, as well their role in the kidney–heart axis.
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Angelica Beate Winter Boldt;
Ewalda Von Rosen Seeling Stahlke;
Thirumalaisamy P. Velavan;
Steffen Thiel;
Iara J. T. Messias-Reason;
Camila de Freitas Oliveira;
Gabriela Canalli Kretzschmar;
Hellen Weinschütz Mendes;
Sérvio Túlio Stinghen;
Fabiana Antunes de Andrade;
Valéria Bumiller Bini;
Letícia B. Gonçalves;
Anna Carolina de Moraes Braga
Palavra-chave:
HBV;
hanseníase;
complemento;
CR1;
via das lectinas;
CRIg
Áreas do conhecimento:
Ciências Biológicas; Genética Humana e Médica;
Ciências Biológicas; Genética; Genética Humana e Médica; Imunogenética
resumo ...
Thousands of leprosy patients not only suffer from physical deformities, but also either have or have had hepatitis B virus (HBV) coinfection. Polymorphisms of the complement system modulate susceptibility to leprosy, but genetic susceptibility to past or present HBV infection is unknown. We used sequencing and multiplex sequence-specific PCR to genotype 72 polymorphisms of seven genes (MBL2, FCN1, FCN2, FCN3, MASP1, MASP2, C3) encoding components of the lectin pathway, and two genes encoding complement receptors (CR1, VSIG4) in 190 patients, of which 74 were positive for HBsAg and/or anti-HBc (HBV+, 93.2% with a resolved infection) and 116 lepromatous patients, and 408 HBV-blood donors. In addition, we tested for levels of proteins of the lectin pathway. We found no difference between serum concentrations of mannan-binding lectin (MBL), MBL-associated serine proteins (MASP-1, MASP-2, MASP-3, MAp44), ficolin-3 (FCN-3), soluble complement receptor 1 (sCR1) and MBL mediated C4 activation, measured by ELISA or TRIFMA in up to 167 HBV+ and HBV− patients. Haplotypes lowering protein levels or encoding dysfunctional proteins increased susceptibility to HBV infection: MBL2*LYQC (OR = 3.4, p = 0.02), MASP1*AC_CC (OR = 4.0, p = 0.015) and MASP2*1C2-l (OR = 5.4, p = 0.03). Conversely, FCN1*3C2 haplotype, associated with higher gene expression, was protective (OR = 0.56, P = 0.033). Other haplotypes associated with HBV susceptibility were: MASP2*2B1-i (OR = 19.25, P = 0.003), CR1*3A ...
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LÓPEZ-BARRERA, ELLIE ANNE;
Grötzner, Sonia Regina;
ESQUIVEL, LUÍSE;
VOIGT, CARMEN LÚCIA;
CAMPOS, SANDRO XAVIER;
C A Oliveira Ribeiro
Palavra-chave:
biomarcadores;
histopatologia;
Rim;
Fígado;
Exposição oral;
bioensaios
Áreas do conhecimento:
Ciências Biológicas; Ecologia Aplicada; Ecotoxicologia
resumo ...
(en)
The studies about silver nanoparticles (AgNP) increased in the last years but few is known about their effects in Brazilian neotropical freshwater fish species. The current study investigated the effects of AgNP on adult silver catfish Rhamdia quelen after subchronic oral exposure. After nanoparticle (NP) size and area characterization fish were administrated with three different doses for 15 days (0.03, 0.3 and 3 μg g-1). The concentration of silver in liver and kidney was measured to evaluate the bioaccumulation and discuss its effects in the target organs. Liver bioaccumulated 15, 1.7 and 0.2 % of administered doses while kidney bioaccumulated 1.33, 0.33 and 0.9 % (respectively for 0.03, 0.3 and 3 μg g-1). The histopathological findings were considered in both organs to evaluate the effects of AgNP, according to Bernet’s Lesion Index (BLI). Also were included the melano-macrophages center (MMC) and new nephrons (NN) counting respectively in liver and posterior kidney. The results revealed morphological injuries as inflammation in both studied organs and vascular congestion and steatosis in liver, in a concentration dependent way. The presence of AgNP in the tissues revealed the bioavailability of the nanoparticle while the damages and morphological disturbs showed the potential risk of exposure in R. quelen, even under environmental relevant concentrations.
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NUNES, KELLY;
Llop, Elena;
Luiza Tamie Tsuneto;
Francisco Mauro Salzano;
Rothhammer, Francisco;
SINGLE, RICHARD;
RUIZ-LINARES, ANDRÉS;
ROCHA, JORGE;
Meyer, Diogo;
MAIA, MARIA HELENA THOMAZ;
DOS SANTOS, EDUARDO JOSÉ MELO;
DOS SANTOS, SIDNEY EMANUEL BATISTA;
GUERREIRO, JOÃO FARIAS;
Maria Luiza Petzl-Erler;
Bedoya, Gabriel;
Gallo, Carla;
Poletti, Giovanni
Palavra-chave:
natural selection;
MHC;
Native American;
Amerindians;
population genetics
Áreas do conhecimento:
Ciências Biológicas; Genética; Imunogenética; Doenças Auto Imunes;
Ciências Biológicas; Genética; Genética Humana e Médica; Imunogenética;
Ciências Biológicas; Bioquímica; Biologia Molecular
resumo ...
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Margarete Johnson;
OTUKI, M;
CABRINI, D;
Ross Rudolph;
Deborah Witherden;
Wendy Havran
Palavra-chave:
gamma delta T cell;
Hspa8;
ICAM-1
Áreas do conhecimento:
Ciências Biológicas; Imunologia Celular;
Ciências Biológicas; Farmacologia; Farmacologia Autonômica; Inflamação
resumo ...
Abstract. Tissue-resident γδ T cells form the first line of defense at barrier surfaces where they survey host tissue for signs of stress or damage. Following r
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M. Johnson;
OTUKI, M;
Daniela Almeida Cabrini;
R. Rudolph;
Deborah Witherden;
Wendy Havran
Palavra-chave:
γδ T cell;
ICAM;
Hspa8;
Wound healing
Áreas do conhecimento:
Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia;
Ciências Biológicas; Farmacologia; Farmacologia Geral
resumo ...
Abstract. Tissue-resident γδ T cells form the first line of defense at barrier surfaces where they survey host tissue for signs of stress or damage. Following r
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Francisco Grotta Neto;
PERES, PEDRO H F;
PIOVEZAN, UBIRATAN;
Fernando C. Passos;
José Mauricio Barbanti Duarte
Palavra-chave:
rabies;
Chiroptera;
Exotic Species
Áreas do conhecimento:
Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia;
Ciências Biológicas; Ecologia; Ecologia de Ecossistemas
resumo ...
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