Publicações - ciências biológicas
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DE FIGUEIREDO, TATIANA ZIEMNICZAK PEREIRA;
VOLL, FERNANDO AUGUSTO PEDERSEN;
KRIEGER, NADIA;
MITCHELL, DAVID ALEXANDER
Palavra-chave:
lipase;
specificity constant;
Selectivity;
transesterification
Áreas do conhecimento:
Engenharias; Engenharia Química; Processos Industriais de Engenharia Química; Processos Bioquímicos;
Ciências Biológicas; Bioquímica; Enzimologia; Cinética Enzimática
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Tatiana Ziemniczak Pereira de Figueiredo;
Fernando Augusto Pedersen Voll;
KRIEGER, NADIA;
MITCHELL, DAVID ALEXANDER
Palavra-chave:
lipase;
seletividade;
constante de especificidade;
diol
Áreas do conhecimento:
Ciências Biológicas; Bioquímica; Bioquímica dos Microorganismos; Biotecnologia;
Ciências Biológicas; Bioquímica; cinética enzimática
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DOS SANTOS-SILVA, DOMINGOS LUCAS;
Jefferson Prado;
EISENLOHR, PEDRO V.
Palavra-chave:
Cerrado-Amazônia transition area;
Phytogeography;
Rocky outcrops;
Rupicolous plants
Áreas do conhecimento:
Ciências Biológicas; Botânica; Ecologia de Pteridófitas; Ecologia de Pteridófitas
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RODRIGUES, ANA CAROLINA;
ZAMBALDE, ERIKA PEREIRA;
BELLAN, DANIEL DE LIMA;
TRINDADE, EDVALDO DA SILVA;
RIBEIRO, ENILZE MARIA DE SOUZA FONSECA;
CALIN, GEORGE;
Daniela Fiori Gradia;
CARVALHO DE OLIVEIRA, JAQUELINE
Palavra-chave:
lnc-uc.147;
uc.147;
T-UCRs;
liver cancer;
lncRNAs
Áreas do conhecimento:
Ciências Biológicas; Genética Humana e Médica
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Lnc-uc.147, a long non-coding RNA derived from a transcribed ultraconserved region (T-UCR), was previously evidenced in breast cancer. However, the role of this region in other tumor types was not previously investigated. The present study aimed to investigate lnc-uc.147 in different types of cancer, as well as to suggest lnc-uc.147 functional and regulation aspects. From solid tumor datasets analysis of The Cancer Genome Atlas (TCGA), deregulated lnc-uc.147 expression was associated with the histologic grade of hepatocellular carcinoma, and with the tumor stage of clear cell renal and gastric adenocarcinoma. Considering the epidemiologic relevance of liver cancer, silencing lnc-uc.147 reduced the viability and clonogenic capacity of HepG2 cell lines. Additionally, we suggest a relation between the transcription factor TEAD4 and lnc-uc.147 in liver and breast cancer cells.
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MATHIAS, CAROLINA;
Daniela Fiori Gradia;
AOKI, MATEUS NÓBREGA;
CARVALHO DE OLIVEIRA, JAQUELINE;
MARIN, ANELIS MARIA;
KOHLER, ANA FLÁVIA;
SANCHUKI, HELOISA BRUNA SOLIGO;
SUKOW, NATALIE;
BELTRAME, MARCIA HOLSBACH;
BAAL, SUELEN CRISTINA SOARES;
Sebastião, Ana Paula Martins;
de Souza Fonseca Ribeiro, Enilze Maria
Palavra-chave:
rs3803662;
rs4415084;
rs4784227;
rs7716600;
breast cancer susceptibility
Áreas do conhecimento:
Ciências Biológicas; Genética Humana e Médica; Genética Humana e Médica
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Long noncoding RNAs (lncRNAs) are a class of non-coding RNAs that contain more than 200 nucleotides and exhibit a versatile regulatory capacity. Genomic alterations in lncRNAs have already been investigated in several complex diseases, including breast cancer (BC). BC is a highly heterogeneous disease and is the most prevalent cancer type among women worldwide. Single nucleotide polymorphisms (SNPs) in lncRNA regions appear to have an important role in BC susceptibility; however, little is known about lncRNA-SNPs in the Brazilian population. This study used Brazilian tumor samples to identify lncRNA-SNPs with a biological role in BC development. We applied a bioinformatic approach intersecting lncRNAs that are differentially expressed in BC tumor samples using The Cancer Genome Atlas (TCGA) cohort data and looked for lncRNAs with SNPs associated with BC in the Genome Wide Association Studies (GWAS) catalog. We highlight four lncRNA-SNPs—rs3803662, rs4415084, rs4784227, and rs7716600—which were genotyped in Brazilian BC samples in a case-control study. The SNPs rs4415084 and rs7716600 were associated with BC development at higher risk. These SNPs were also associated with progesterone status and lymph node status, respectively. The rs3803662/rs4784227 haplotype GT was associated with BC risk. These genomic alterations were also evaluated in light of the lncRNA’s secondary structure and gain/loss of miRNA binding sites to better understand its biological functions. We emphasize that our bioinformatics approach could find lncRNA-SNPs with a potential biological role in BC development and that lncRNA-SNPs should be more deeply investigated in a highly heterogeneous disease population.
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AOYAGI, LEONARDO MURILO;
BARACHO, DOUGLAS HENRIQUE;
Ana Teresa Lombardi
Palavra-chave:
Bioquímica;
biotecnologia microalgas;
carbohydrate;
cobre;
ecofisiologia microalgas
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Microalgae are a natural source of valuable compounds with a wide range of applications. Given their physiological plasticity, strategies aiming at increasing the content of biomolecules have been proposed. In this study, low-dose copper and blue/red lights were combined and used as a manipulative strategy to induce biomolecule accumulation in Kirchneriella contorta. Cultures were exposed to a 1.6 × 10–9 to 1.7 × 10–8 mol L−1 free copper (Cu2+) concentration range under white light for 48 h. Afterwards, the white light was replaced with blue or red lights for up to 96 h. We evaluated population growth through growth rates, photosynthesis through pulse amplitude modulated fluorescence (PAM) and biomolecules by quantifying chlorophyll a, carotenoids, carbohydrates and proteins. The results showed that at 3.8 × 10–9 mol L−1 Cu2+ and blue light, 2 × more carotenoids, 4 × more carbohydrates and 3 × more proteins were accumulated in the cells compared to the control. Among the light colors, the blue light most significantly interfered with the algal metabolism compared to the other colors. Although growth rates and maximum and operational quantum yields were not affected, non-photochemical quenching (NPQ) increased under blue light, showing that its higher energy compared to red and white lights may have impacted the photosynthetic apparatus. This study contributes to the biotechnology of microalgae by proposing a manipulative strategy that triggered the build-up of biomolecules in K. contorta.
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DA ROSA, MICHELLE DIAS HORNES;
ALVES, CRISTINA JANSEN;
DOS SANTOS, FELIPE NARDO;
DE SOUZA, ALEXANDER OSSANES;
ZAVAREZE, ELESSANDRA DA ROSA;
PINTO, ERNANI;
Miguel Noseda;
RAMOS, DANIELA;
DE PEREIRA, CLÁUDIO MARTIN PEREIRA
Palavra-chave:
macroalgae;
Microalgae;
Biomass
Áreas do conhecimento:
Ciências Biológicas; Bioquímica; Biotecnologia
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DINIZ FILHO, JOSE ALEXANDRE F.;
Cláudio J B Carvalho;
LADLE, RICHARD J.;
HORTAL, JOAQUIN;
JARDIM, LUCAS;
GUEDES, JHONNY J.M.;
MEYER, LEILA;
STROPP, JULIANA;
FRATELES, LIVIA ESTÉFANE FERNANDES;
PINTO, RAFAEL B.;
Lohmann, Lúcia G.;
TESSAROLO, GEIZIANE
Palavra-chave:
biodiversity bias;
Darwinian shortfall;
latitudinal taxonomy gradient;
Linnean shortfall;
macroecology;
Wallacean shortfall
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DE AZEVEDO, ALEXANDRE LUIZ KORTE;
CARVALHO, TAMYRES MINGORANCE;
MARA, CRISTIANE SATO;
GINER, IGOR SAMESIMA;
DE OLIVEIRA, JAQUELINE CARVALHO;
Daniela Fiori Gradia;
CAVALLI, IGLENIR JOÃO;
RIBEIRO, ENILZE M. S. F.
Palavra-chave:
Breast cancer metastasis;
Feed-forward loops;
micro-RNAs;
Cancer therapy;
Network analysis
Áreas do conhecimento:
Ciências Biológicas; Genética Humana e Médica
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Metastasis is a multi-step process that leads to the dissemination of tumor cells to new sites and, consequently, to multi-organ neoplasia. Although most lethal breast cancer cases are related to metastasis occurrence, little is known about the dysregulation of each step, and clinicians still lack reliable therapeutic targets for metastasis impairment. To fill these gaps, we constructed and analyzed gene regulatory networks for each metastasis step (cell adhesion loss, epithelial-to-mesenchymal transition, and angiogenesis). Through topological analysis, we identified E2F1, EGR1, EZH2, JUN, TP63, and miR-200c-3p as general hub-regulators, FLI1 for cell-adhesion loss specifically, and TRIM28, TCF3, and miR-429 for angiogenesis. Applying the FANMOD algorithm, we identified 60 coherent feed-forward loops regulating metastasis-related genes associated with distant metastasis-free survival prediction. miR-139-5p, miR-200c-3p, miR-454-3p, and miR-1301-3p, among others, were the FFL’s mediators. The expression of the regulators and mediators was observed to impact overall survival and to go along with metastasis occurrence. Lastly, we selected 12 key regulators and observed that they are potential therapeutic targets for canonical and candidate antineoplastics and immunomodulatory drugs, like trastuzumab, goserelin, and calcitriol. Our results highlight the relevance of miRNAs in mediating feed-forward loops and regulating the expression of metastasis-related genes. Altogether, our results contribute to understanding the multi-step metastasis complexity and identifying novel therapeutic targets and drugs for breast cancer management.
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ANDRADE, MARCOS K.;
SOUZA, LEONARDO C.;
AZEVEDO, EVELLYN M.;
BAIL, ELLEN L.;
ZANATA, SILVIO M.;
ANDREATINI, R;
maria vital
Palavra-chave:
melatonin;
Alzheimer's disease;
streptozotocin;
APP
Áreas do conhecimento:
Ciências Biológicas; Farmacologia; Neuropsicofarmacologia; Doenças Neurodegenerativas;
Ciências Biológicas; Farmacologia; Neuropsicofarmacologia
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