Publicações - ciências biológicas
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KOLB, KATHLEEN LIEDTKE;
HUNDT, JENNIFER ELISABETH;
SHULDINER, ALAN R.;
LOPES, FABIANA LEÃO;
BOYSEN, TEIDE-JENS;
FRANKE, ANDRE;
PINTO, LUIS FELIPE RIBEIRO;
SOARES-LIMA, SHEILA COELHO;
KRETZSCHMAR, GABRIELA CANALLI;
Angelica Beate Winter Boldt;
MIRA, ANA LUIZA SPROTTE;
AUER, EDUARDO DELABIO;
BUCCO, ISABELA DALL?OGLIO;
DE LIMA E SILVA, CARLA EDUARDA;
DOS SANTOS, PRISCILA IANZEN;
HOCH, VALÉRIA BUMILLER-BINI;
OLIVEIRA, LUANA CAROLINE;
HAUSER, ALINE BORSATO
Palavra-chave:
glucocorticoide;
GR;
eixo HPA;
síndrome metabólica;
menonita;
metilação do DNA
Áreas do conhecimento:
Ciências Biológicas; Genética Humana e Médica
resumo ...
The regulation of the hypothalamic-pituitary-adrenal (HPA) axis is associated with polymorphisms and the methylation degree of the glucocorticoid receptor gene (NR3C1) and is potentially involved in the development of metabolic syndrome (MetS). In order to evaluate the association between MetS with the polymorphisms, methylation, and gene expression of the NR3C1 in the genetically isolated Brazilian Mennonite population, we genotyped 20 NR3C1 polymorphisms in 74 affected (MetS) and 138 unaffected individuals without affected first-degree relatives (Co), using exome sequencing, as well as five variants from non-exonic regions, in 70 MetS and 166 Co, using mass spectrometry. The methylation levels of 11 1F CpG sites were quantified using pyrosequencing (66 MetS and 141 Co), and the NR3C1 expression was evaluated via RT-qPCR (14 MetS and 25 Co). Age, physical activity, and family environment during childhood were associated with MetS. Susceptibility to MetS, independent of these factors, was associated with homozygosity for rs10482605*C (OR = 4.74, pcorr = 0.024) and the haplotype containing TTCGTTGATT (rs3806855*T_ rs3806854*T_rs10482605*C_rs10482614*G_rs6188*T_rs258813*T_rs33944801*G_rs34176759*A_rs17209258*T_rs6196*T, OR = 4.74, pcorr = 0.048), as well as for the CCT haplotype (rs41423247*C_ rs6877893*C_rs258763*T), OR = 6.02, pcorr = 0.030), but not to the differences in methylation or gene expression. Thus, NR3C1 polymorphisms seem to modulate the susceptibility to MetS in Mennonites, independently of lifestyle and early childhood events, and their role seems to be unrelated to DNA methylation and gene expression.
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LIMA-KEPPE, ISABELA;
SAITO, VICTOR SATORU;
SOARES, JAQUELINE FERNANDA;
NUNES-SILVA, ALINE;
Antonio Ostrensky Neto;
FERREIRA, IURI EMMANUEL DE PAULA;
PERBICHE-NEVES, GILMAR
Palavra-chave:
zooplâncton;
ecologia aquática;
Controle de larvas
Áreas do conhecimento:
Ciências Biológicas; Ecologia; Ecologia Aplicada;
Engenharias; Engenharia Mecânica; Engenharia Térmica; Controle Ambiental;
Ciências Biológicas; Zoologia; Zoologia Aplicada; Controle Populacional de Animais
resumo ...
How can the copepod Mesocyclops longisetus (Thiébaud, 1912) be used to control mosquito production effectively in pots, plates, and slabs? - Volume 39
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Aliciane de Almeida Roque;
Francisco Filipack Neto;
Claudia Cosio;
Iris Barjhoux;
C A Oliveira Ribeiro;
Damien Rioult
Palavra-chave:
Imunotoxicology;
Pesticidas;
macrófago;
ecotoxicologia;
cultivo celular
Áreas do conhecimento:
Ciências Biológicas; Ecologia Aplicada; Ecotoxicologia;
Ciências Biológicas; Morfologia; Citologia e Biologia Celular; Toxicologia Celular
resumo ...
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Rodgher S.;
Bruna Horvath Vieira;
Renata N. Haneda;
Ana Teresa Lombardi;
Maria Da Graça Gama Melão;
Michiel Adriaan Daam
Palavra-chave:
Algas;
ecologia;
ecotoxicologia
Áreas do conhecimento:
Ciências Biológicas; Ecologia; Ecologia Aplicada
resumo ...
Metal pollution of aquatic ecosystems continues to be a worldwide concern, and many studies have previously been conducted evaluating metal toxicity to aquatic organisms. However, these studies have mainly been focused on temperate species and only evaluated aqueous exposure, whereas aquatic organisms under real-world conditions are also exposed to metals through their diet. Toxicity tests were conducted evaluating the effects of Cr on cell density and biochemical composition of the microalga Chlorella vulgaris. Additionally, the toxic effects of Cr on the temperate Ceriodaphnia dubia and the tropical Ceriodaphnia silvestrii cladocerans were investigated through three contamination routes: contaminated medium (CM), contaminated food (CF; C. vulgaris), and the combination of both situations (CMF; contaminated medium and food). The toxicity tests with the cladocerans evaluated the metal effects on survival, number of newborns, and feeding activity. Exposure to 48 µg L−1 total dissolved Cr reduced cell density and increased protein, carbohydrate, and lipid content in C. vulgaris. The increase in the biochemical composition and hence food quality of the microalgae after Cr exposure have contributed to the absence of toxic effects to the cladocerans in the CF treatment. No toxic effects were observed to the temperate cladoceran at any treatment. The tropical cladoceran C. silvestrii showed reduced filtration, ingestion and reproduction rates in the CM and CMF treatments at a Cr concentration below national and international standards. Implications for considering different exposure pathways in ecotoxicological studies, (tropical) risk assessments and indications for future research are discussed.
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Raul Czelujinski Varella;
Ângelo Parise Pinto
Palavra-chave:
Antactoperlaria;
Aquatic insects;
Neotropical;
Systematics;
Taxonomy
Áreas do conhecimento:
Ciências Biológicas; Zoologia; Morfologia dos Grupos Recentes;
Ciências Biológicas; Zoologia; Taxonomia dos Grupos Recentes
resumo ...
ABSTRACT A new species of Gripopteryginae stonefly, Tupiperla claudius sp. nov. (holotype male...
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ABREU, HELLEN;
BAPTISTA, PATRÍCIA ALVAREZ DA SILVA;
BONATTO, ANA CLAUDIA;
Daniela Fiori Gradia;
ADAMOSKI, DOUGLAS;
MALTEMPI DE SOUZA, EMANUEL;
CARVALHO DE OLIVEIRA, JAQUELINE;
ROYER, CARLA ADRIANE;
POITEVIN, CAROLINA GRACIA;
KOHLER, ANA FLÁVIA;
RODRIGUES, ANA CAROLINA;
RABONI, SONIA MARA;
NOGUEIRA, MERI BORDIGNON;
CARDOSO, PEDRO HENRIQUE;
ARRUDA, MONICA BARCELLOS
Palavra-chave:
virus detection;
Diagnostic Techniques and Procedures;
Surveillance;
flu;
COVID-19
resumo ...
After the Coronavirus pandemic, the importance of virus surveillance was highlighted, reinforcing the constant necessity of discussing and updating the methods for collection and diagnoses, including for other respiratory viruses. Although the nasopharyngeal swab is the gold-standard sample for detecting and genotyping SARS-CoV-2 and Influenza viruses, its collection is uncomfortable and requires specialized teams, which can be costly. During the pandemic, non-invasive saliva samples proved to be a suitable alternative for SARS-CoV-2 diagnosis, but for Influenza virus the use of this sample source is not recognized yet. In addition, most SARS-CoV-2 comparisons were conducted before the Omicron variant emerged. Here, we aimed to compare Influenza A and Omicron RT-qPCR analysis of nasopharyngeal swabs and saliva self-collection in paired samples from 663 individuals. We found that both nasopharyngeal swab and saliva collection are efficient for the diagnosis of Omicron (including sub-lineages) and for Influenza A, with high sensitivity and accuracy (>90%). The kappa index is 0.938 for Influenza A and 0.905 for SARS-CoV-2. These results showed excellent agreement between the two samples reinforcing saliva samples as a reliable source for detecting Omicron and highlighting saliva as a valid sample source for Influenza detection, considering this cheaper and more comfortable alternative.
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SANTOS, LUCINHA;
André Martins Vaz dos Santos
Palavra-chave:
sagitta;
habitat;
Habit;
otolith morphological features;
sample size
Áreas do conhecimento:
CIENCIAS_AGRARIAS; Zootecnia e Recursos Pesqueiros;
Ciências Exatas e da Terra; Oceanografia; Oceanografia Biológica; Interação entre os Organismos Marinhos e os Parâmetros Ambientais;
Ciências Biológicas; Zoologia; Zoologia Aplicada; Conservação das Espécies Animais
resumo ...
The sagitta otoliths of teleostean fishes are usually used in diverse studies. Their shapes are species-specific, and the qualitative description of their morphological features seems to be a simple task, despite its subjectivity. On the other hand, morphometric techniques have been developed with a focus on objectivity, reproducibility, and accuracy. Considering this, the otoliths morphology was reviewed and evaluated in terms of robustness as a taxonomic tool and to highlight ecomorphological patterns. The otoliths morphology of 179 teleostean fishes from the Southern Atlantic were analyzed. For each species, the fish total length range, eighteen otolith morphological features (OMFs), and relative proportions were annotated. Species habitat and habit were also recorded. Data analyses were based on exploratory analysis, correlation, nonmetric multidimensional scaling, and a two-way permutational analysis of variance. The most descriptive OMFs were: colliculum, sulcus acusticus (morphology, position, orientation, and opening), and otolith profile. The otolith morphology was significantly related to species habitat and habit, with a new pattern described for deep-ocean pelagic species. In conclusion, otoliths morphology is robust whenever it is based on the comparative method application (otoliths among otoliths), considering the constant updates of fishes’ taxonomy and the use of proper sample sizes linked to morphometric techniques.
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Estevan Luiz da Silveira;
SEMMAR, NABIL;
Eduardo Luis Cupertino Ballester;
André Martins Vaz dos Santos
Palavra-chave:
stomach content analysis;
percentages of occurrence;
multivariate analysis;
feeding trends;
fish community interactions
Áreas do conhecimento:
CIENCIAS_AGRARIAS; Recursos Pesqueiros e Engenharia de Pesca; Recursos Pesqueiros de Águas Interiores;
Ciências Biológicas; Zoologia; Zoologia Aplicada; Conservação das Espécies Animais;
Ciências Biológicas; Ecologia; Ecologia Aplicada
resumo ...
Feeding ecology is an integrative procedure to highlight different diets, associating feeding trends with governing and regulation factors characterizing foraging species and their environments, respectively. The diet variability of seven fish species forming a community in a Neotropical riverine system was analyzed to characterize the resource and consumer linkages, providing a detailed assessment of adaptive feeding behaviors of fishes living in different ecological states transiting from natural/resilient spaces to anthropic pressions-linked disturbed ones. Fishes were sampled along four sites during a year, and their stomach contents were analyzed. Feeding data were analyzed by applying quali- and quantitative methods with multi-levels and multifactor aspects to determine target food categories (percentage of occurrence) and identify feeding patterns (correspondence and cluster analyses, CA-HCA). Factors and scales governing target food categories were also tested. A total of 483 stomachs were dissected, and 30 food categories were identified. CA-HCA highlighted 10 feeding trends (FTs) combining several foods co-occurring at distinct levels. These FTs indicated characteristic diets and high diversity of feeding behaviors concerning multiple and narrow diets, different alimentary needs related to ecomorphological features, different plasticity ranges (adaptability, tolerance) and a spatial governing effect (headwater to river mouth environmental quality loss). These multiple factors provided essential information on overcoming ways of environmental constraints and optimization ways of food balances helping to better manage the richness and working of neotropical river systems.
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Martins, M. A.;
Débora Rasec Raduslki;
Thiago Rodrigues da Silva;
Ana Maria Raymundi;
Cristina Aparecida Jark Stern;
Aleksander Roberto Zampronio
Palavra-chave:
Condicionamento;
sepse;
Hipocampo Dorsal
Áreas do conhecimento:
Ciências Biológicas; Farmacologia; Neuropsicofarmacologia
resumo ...
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RODRIGUES, ANA CAROLINA;
ZAMBALDE, ERIKA PEREIRA;
BELLAN, DANIEL DE LIMA;
TRINDADE, EDVALDO DA SILVA;
RIBEIRO, ENILZE MARIA DE SOUZA FONSECA;
CALIN, GEORGE;
Daniela Fiori Gradia;
CARVALHO DE OLIVEIRA, JAQUELINE
Palavra-chave:
lnc-uc.147;
uc.147;
T-UCRs;
liver cancer;
lncRNAs
Áreas do conhecimento:
Ciências Biológicas; Genética Humana e Médica
resumo ...
Lnc-uc.147, a long non-coding RNA derived from a transcribed ultraconserved region (T-UCR), was previously evidenced in breast cancer. However, the role of this region in other tumor types was not previously investigated. The present study aimed to investigate lnc-uc.147 in different types of cancer, as well as to suggest lnc-uc.147 functional and regulation aspects. From solid tumor datasets analysis of The Cancer Genome Atlas (TCGA), deregulated lnc-uc.147 expression was associated with the histologic grade of hepatocellular carcinoma, and with the tumor stage of clear cell renal and gastric adenocarcinoma. Considering the epidemiologic relevance of liver cancer, silencing lnc-uc.147 reduced the viability and clonogenic capacity of HepG2 cell lines. Additionally, we suggest a relation between the transcription factor TEAD4 and lnc-uc.147 in liver and breast cancer cells.
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