Publicações - ciências biológicas
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Miúriel de Aquino Goulart;
VAZ, FREDERICO FONTANELLI;
OLIVEIRA KOCH, MARÍLIA DE;
Carlos Czpak Kroetz;
Rosangela Locatelli Dittrich
Palavra-chave:
hematologia aves;
blood parameter;
aves silvestres
Áreas do conhecimento:
Ciências Biológicas; Medicina Veterinária
resumo ...
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PEREIRA ZAMBALDE, ERIKA;
MATHIAS, CAROLINA;
RODRIGUES, ANA CAROLINA;
SOUZA FONSECA RIBEIRO, ENILZE M.;
Daniela Fiori Gradia;
CALIN, GEORGE A.;
CARVALHO DE OLIVEIRA, JAQUELINE
Palavra-chave:
T-UCRs;
cancer;
Diseases;
long noncoding RNA;
transcribed ultraconserved regions
resumo ...
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SERENESKI-LIMA, CAROLINA;
BAGGIO, RAFAEL ANTUNES;
Pil, M. W.;
Torres Boeger, Maria Regina;
Walter Antonio Boeger
Palavra-chave:
manguezal;
Biogeografia;
filogeografia
Áreas do conhecimento:
Ciências Biológicas; Filogeografia;
Ciências Biológicas; Zoologia; Evolução
resumo ...
Laguncularia racemosa (L.) Gaertn (Combretaceae) is one of the eight tree mangrove species of the Atlantic-Caribbean-Eastern Pacific region (AEP). Thi…
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DESIDERATO, ANDREA;
Marcos Soares Barbeitos;
GILBERT, CLÉMENT;
DA LAGE, JEAN-LUC
Palavra-chave:
Alpha-amylase;
Gene loss;
Horizontal gene transfer
Áreas do conhecimento:
Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia
resumo ...
Abstract. The subfamily GH13_1 of alpha-amylases is typical of Fungi, but it is also found in some unicellular eukaryotes (e.g., Amoebozoa, choanoflagellates) a
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Hercules Tancredo Moreira;
Luciana Grange;
ISAC GEORGE ROSSET;
Robson F. Missio
Palavra-chave:
BNF;
HPLC;
Bacteria;
Triticum aestivum
Áreas do conhecimento:
Ciências Biológicas; Biotecnologia Vegetal
resumo ...
(en)
In this study, we identified the presence and quantity of flavonoids produced in wheat roots inoculated with rhizobacteria. Our goal is to confirm the efficiency of standard isolates and show new strains with biotechnological potential to promote plant growth. The experiment was carried out with different isolates under inoculation in the following situations: T1-Azospirillum brasiliense; T2-Herbaspirillum seropedicae; T3-Azospirillum brasiliense and Herbaspirillum seropedicae co-inoculation; T4-native Enterobacter sp. nº 203; T5- native Enterobacter sp. nº 208; T6-native Enterobacter sp. nº 493; T7-Control only under nitrogen fertilization (N+); T8-Control without nitrogen (N-) and bacterial inoculation. Agronomic characteristics were assessed after 42 days of inoculation. Identification and quantification of flavonoids were carried out through HPLC, using an analytical curve with four standards based on Coumarin, Quercetin, Isoflavone and Rutin. Regarding the production of total flavonoids, two (203 and 493) out of the three native strains we tested were statistically significant, exceeding the values obtained from the inoculation of standard strains, which presented association with grasses (Azopirilum e Herbaspirillum). Standard bacteria, when inoculated in isolation, presented, along with those that received N+ treatment, the highest values for length and root and aerial part dry mass. New studies need to be carried out in order to confirm the technological use of these native strains as inoculant, as these bacteria may contribute to Biological Nitrogen Fixation (BNF) in wheat culture, either by competition or synergism.
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OZAWA, PATRICIA M. M.;
URBAN, CÍCERO DE ANDRADE;
KURODA, FLAVIA;
LIMA, RUBENS S.;
ALMEIDA, RODRIGO C.;
GRADIA, DANIELA F.;
CAVALLI, IGLENIR J.;
CAVALLI, LUCIANE R.;
MALHEIROS, DANIELLE;
RIBEIRO, ENILZE M. S. F.;
VIEIRA, EVELYN;
LEMOS, DÉBORA S.;
SOUZA, INGRID L. MELO;
Silvio Marques Zanata;
PANKIEVICZ, VÂNIA C.;
TULESKI, THALITA R.;
SOUZA, EMANUEL M.;
WOWK, PRYSCILLA F.
Palavra-chave:
cancer biology;
Vesícula extracelular
Áreas do conhecimento:
Ciências Biológicas; Morfologia; Citologia e Biologia Celular
resumo ...
MicroRNAs derived from extracellular vesicles (EV-miRNAs) are circulating miRNAs considered as potential new diagnostic markers for cancer that can be easily detected in liquid biopsies. In this study, we performed RNA sequencing analysis as a screening strategy to identify EV-miRNAs derived from serum of clinically well-annotated breast cancer (BC) patients from the south of Brazil. EVs from three groups of samples (healthy controls (CT), luminal A (LA), and triple-negative (TNBC)) were isolated from serum using a precipitation method and analyzed by RNA-seq (screening phase). Subsequently, four EV-miRNAs (miR-142-5p, miR-150-5p, miR-320a, and miR-4433b-5p) were selected to be quantified by quantitative real-time PCR (RT-qPCR) in individual samples (test phase). A panel composed of miR-142-5p, miR-320a, and miR-4433b-5p distinguished BC patients from CT with an area under the curve (AUC) of 0.8387 (93.33% sensitivity, 68.75% specificity). The combination of miR-142-5p and miR-320a distinguished LA patients from CT with an AUC of 0.9410 (100% sensitivity, 93.80% specificity). Interestingly, decreased expression of miR-142-5p and miR-150-5p were significantly associated with more advanced tumor grades (grade III), while the decreased expression of miR-142-5p and miR-320a was associated with a larger tumor size. These results provide insights into the potential application of EVs-miRNAs from serum as novel specific markers for early diagnosis of BC.
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OZAWA, PATRICIA M. M.;
URBAN, CÍCERO DE ANDRADE;
KURODA, FLAVIA;
LIMA, RUBENS S.;
ALMEIDA, RODRIGO C.;
Daniela Fiori Gradia;
CAVALLI, IGLENIR J.;
CAVALLI, LUCIANE R.;
MALHEIROS, DANIELLE;
RIBEIRO, ENILZE M. S. F.;
VIEIRA, EVELYN;
LEMOS, DÉBORA S.;
SOUZA, INGRID L. MELO;
ZANATA, SILVIO M.;
PANKIEVICZ, VÂNIA C.;
TULESKI, THALITA R.;
SOUZA, EMANUEL M.;
WOWK, PRYSCILLA F.
Palavra-chave:
RNA-seq;
circulating microRNAs;
extracellular vesicles;
liquid biopsy;
miRNAs
Áreas do conhecimento:
Ciências Biológicas; Genética Humana e Médica
resumo ...
MicroRNAs derived from extracellular vesicles (EV-miRNAs) are circulating miRNAs considered as potential new diagnostic markers for cancer that can be easily detected in liquid biopsies. In this study, we performed RNA sequencing analysis as a screening strategy to identify EV-miRNAs derived from serum of clinically well-annotated breast cancer (BC) patients from the south of Brazil. EVs from three groups of samples (healthy controls (CT), luminal A (LA), and triple-negative (TNBC)) were isolated from serum using a precipitation method and analyzed by RNA-seq (screening phase). Subsequently, four EV-miRNAs (miR-142-5p, miR-150-5p, miR-320a, and miR-4433b-5p) were selected to be quantified by quantitative real-time PCR (RT-qPCR) in individual samples (test phase). A panel composed of miR-142-5p, miR-320a, and miR-4433b-5p distinguished BC patients from CT with an area under the curve (AUC) of 0.8387 (93.33% sensitivity, 68.75% specificity). The combination of miR-142-5p and miR-320a distinguished LA patients from CT with an AUC of 0.9410 (100% sensitivity, 93.80% specificity). Interestingly, decreased expression of miR-142-5p and miR-150-5p were significantly associated with more advanced tumor grades (grade III), while the decreased expression of miR-142-5p and miR-320a was associated with a larger tumor size. These results provide insights into the potential application of EVs-miRNAs from serum as novel specific markers for early diagnosis of BC.
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BRITO, HAISSA OLIVEIRA;
ZAMPRONIO, ALEKSANDER ROBERTO;
RADULSKI, DÉBORA;
WILHELMS, DANIEL BJÖRK;
STOJAKOVIC, ANDREA;
BRITO, LUCIANE MARIA OLIVEIRA;
GIL DA COSTA, RUI MIGUEL;
TRINDADE, EDVALDO S.;
ENGBLOM, DAVID;
FRANCO, CELIA REGINA CAVICHIOLO
Palavra-chave:
febre;
Sistema Imune;
marcadores hipotalâmicos
resumo ...
Lipopolysaccharide (LPS) induces fever through cytokines like receptor-activator of nuclear factor κB ligand (RANKL), triggering mediators like prostaglandins (PG), endothelin-1 (ET-1), corticotrophin-releasing factor (CRF), substance P (SP) and endogenous opioids. LPS-induced fever is reduced in females compared with males except in ovariectomized (OVX) females which show increased fever mediated by PG. The present study aimed to identify the mediators involved in fever in intact and OVX female rats. Fever was induced with LPS (50 μg/kg) intraperitoneally or CRF (2.5 μg), ET-1 (1 pg), morphine (10 μg) and SP (500 ng) intracerebroventricularly in sham-operated and OVX rats. The role of RANKL was evaluated with osteoprotegerin (OPG, 1 μg, intracerebroventricularly). Expression of RANK, CRFI/II, ETB, μ-opioid (MOR) and NK1 receptors was evaluated by confocal microscopy. Besides LPS, only morphine induced fever in OVX rats while all mediators induced fever in sham-operated animals. OPG abolished LPS-induced fever in OVX but not sham-operated animals. Overall, fever involves similar central mediators in cycling females and males but only morphine induced fever in OVX females. Importantly, RANK/RANKL participates in LPS-induced fever in OVX females, as in males but not in cycling females.
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DE ARAÚJO AMATUZZI, JULIO CÉSAR;
DO NASCIMENTO VIEIRA, LEILA;
SANT?ANNA-SANTOS, BRUNO FRANCISCO;
Miguel Noseda;
PACHECO DE FREITAS FRAGA, HUGO
Palavra-chave:
macroalgae;
Seaweed;
Biostimulant activity;
Orchids;
Aqueous extract
Áreas do conhecimento:
Ciências Biológicas; Biotechnology
resumo ...
Orchidaceae is one of the largest plant families and includes species with high economic, ecological, and medicinal importance. Orchid seeds are produced in high amounts; however, these seeds show low germination percentage and have specific germination requirements. To enhance the germination and development of orchids, in vitro techniques have been successfully applied. Natural extracts can be used as a biostimulant in the culture medium, showing improved germination, elongation, and rooting. Thus, the main objective of this study was to determine the effects of Kappaphycus alvarezii aqueous extract in the in vitro development of Epidendrum secundum, evaluating its influence on plantlet morphoanatomy. The K. alvarezii extract was also characterized by nuclear magnetic resonance (NMR) spectroscopy. E. secundum plantlets obtained after the in vitro germination were subjected to in vitro elongation experiment in the presence of K. alvarezii extract (0, 6, 12, 25, 50, and 100 mg L− 1). Our results indicated that the aqueous extract of K. alvarezii has a positive effect, stimulating rooting, plantlet development, fresh mass gain, and newly formed shoots of E. secundum plantlets, mainly at 50 mg L− 1 concentration. The use of K. alvarezii extract indicates potential application in tissue culture and plant production, due to its biostimulating effect on plants. Considering the great market demand for new technologies and the growing interest in the application of marine resources, our results present relevant data on the biostimulating potential of this extract, which can be successfully applied for the cultivation of other species of orchids and species of ecological and economic interest.
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DOBRZANSKI, TATIANE;
POBRE, VÂNIA;
MORENO, LEANDRO FERREIRA;
BARBOSA, HELBA CIRINO DE SOUZA;
Rose Adele Monteiro;
Fábio de Oliveira Pedrosa;
Emanuel Maltempi de Souza;
ARRAIANO, CECÍLIA MARIA;
STEFFENS, M
Palavra-chave:
Herbaspirillum seropedicae;
nitrogen fixation;
ncRNA;
smallRNA
Áreas do conhecimento:
Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia;
Ciências Biológicas; Bioquímica; Biologia Molecular;
Ciências Biológicas; Bioquímica; Bioinformática
resumo ...
Herbaspirillum seropedicae is a diazotrophic bacterium from the β-proteobacteria class that colonizes endophytically important gramineous species, promotes their growth through phytohormone-dependent stimulation and can express nif genes and fix nitrogen inside plant tissues. Due to these properties this bacterium has great potential as a commercial inoculant for agriculture. The H. seropedicae SmR1 genome is completely sequenced and annotated but despite the availability of diverse structural and functional analysis of this genome, studies involving small non-coding RNAs (sRNAs) has not yet been done. We have conducted computational prediction and RNA-seq analysis to select and confirm the expression of sRNA genes in the H. seropedicae SmR1 genome, in the presence of two nitrogen independent sources and in presence of naringenin, a flavonoid secreted by some plants. This approach resulted in a set of 117 sRNAs distributed in riboswitch, cis-encoded and trans-encoded categories and among them 20 have Rfam homologs. The housekeeping sRNAs tmRNA, ssrS and 4.5S were found and we observed that a large number of sRNAs are more expressed in the nitrate condition rather than the control condition and in the presence of naringenin. Some sRNAs expression were confirmed in vitro and this work contributes to better understand the post transcriptional regulation in this bacterium. H. seropedicae SmR1 express sRNAs in the presence of two nitrogen sources and/or in the presence of naringenin. The functions of most of these sRNAs remains unknown but their existence in this bacterium confirms the evidence that sRNAs are involved in many different cellular activities to adapt to nutritional and environmental changes.
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