Publicações - ciências biológicas


Juan J Cruz-Motta; Maritza Cardenas; Rosana Moreira da Rocha; Patricia Miloslavich; Edlin Guerra-Castro; Alejandra Hernández; Cesar Herrera; Francisco Barros; Sergio Navarrete; Roger Sepúlveda; Gregorio Bigatti
JOURNAL OF BIOGEOGRAPHY, v. 47, p. 1966-1979, 2020 DOI
Palavra-chave: costão rochoso; Invertebrados Marinhos; distribuição espacial; Beta diversidade; zona entremarés
Áreas do conhecimento: Ciências Biológicas; Ecologia; Ecologia de Ecossistemas
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Aim We evaluated whether patterns of species diversity (α, β and γ) of rocky shore assemblages followed latitudinal gradients (i.e. LDGs) along the South American coasts, and tested hypotheses relat...
REZENDE, GABRIELA CABRAL; Christoph Knogge; Fernando C. Passos; Gabriela Ludwig; Leonardo de Carvalho Oliveira; Leandro Jerusalinsky; Russell A Mittermeier
THE IUCN RED LIST OF THREATENED SPECIES, v. 2020, p. x-xx, 2020
Palavra-chave: Leontopithecus chrysopugus; Black Lion Tamarin; IUCN Red List
Áreas do conhecimento: Ciências Biológicas; Ecologia; Conservação e Manejo de Fauna; Ciências Biológicas; Zoologia; Zoologia Aplicada; Conservação das Espécies Animais; Ciências Biológicas; Ecologia; Ecologia de Ecossistemas
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MEDINA, CANDELA; LETZKUS, JOHANNES J.; ROMANO, ARTURO; DE LA FUENTE, VERÓNICA; TOM DIECK, SUSANNE; NASSIM-ASSIR, BELQUIS; DALMAY, TAMAS; BARTNIK, INA; Paula Santana Lunardi; DE OLIVEIRA ALVARES, LUCAS; SCHUMAN, ERIN M.
NEUROBIOLOGY OF LEARNING AND MEMORY, v. 173, p. 107275 2020 DOI Home page
Palavra-chave: consolidation; reconsolidation; extinction; cofilin; LIMK; Fear memory
Áreas do conhecimento: Ciências Biológicas; Neuroscience
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Mariana Alejandra Cherman; BASÍLIO, DANIEL S.; Kleber Makoto Mise; Johannes Frisch; Andrew B.T. Smith; Almeida, LM.
ZOOTAXA, v. 4896, p. 46-84, 2020 DOI
Palavra-chave: Diplotaxini; Scarabaeoidea; Systematics
Áreas do conhecimento: Ciências Biológicas; Entomologia; Ciências Biológicas; Zoologia; Taxonomia dos Grupos Recentes
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(en) The biodiversity of Liogenys Guérin-Méneville, 1831 (Coleoptera: Scarabaeidae: Melolonthinae: Diplotaxini) from the southern South American Transition Zone and boundaries is presented. Four new species are described: L. brachyclypeata Cherman, new species ; L. lucialmeidae Cherman, new species ; L. martinezi Cherman, new species ; and L. maxillaricuspis Cherman, new species . The synonymy of L. flaveola Moser, 1924 (= L. kadleci Frey, 1970) is proposed. Lectotypes are designated for L. flavida Moser, 1918; L. pallidicornis Blanchard, 1851 (currently L. xanthocera Harold, 1869); and L. rufoflava Moser, 1918. Redescriptions are provided for all the species mentioned above plus L. calcarata Frey, 1970 and L. kunzteni Moser, 1921, as well as an identification key and updated geographical distributions to all species in the region. All species are present in the Monte province, except of L. kuntzeni (Andean provinces of Chile). Liogenys flavida and L. rufoflava have the broadest distribution, the latter here expanded to Paraguay and Chile.  
Nilce May Turcatti; C A Oliveira Ribeiro; Maristela Azevedo; Garcia, J.R.E.; Angie Thaisa da Costa Souza4; Sonia Grotzner; Elton Celton de Oliveira; Alex Fabiano Paulin; Natalicio Ferreira Leite; Francisco Filipack Neto
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 187, p. 109815 2020 DOI
Palavra-chave: Tribromofenol; Estágios Iniciais de Desenvolvimento; Rhamdia quelen; Modelagem Ecológica
Áreas do conhecimento: Ciências Biológicas; Ecologia Aplicada; Ecotoxicologia; Ciências Biológicas; Zoologia; Zoologia Aplicada; Conservação das Espécies Animais
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Sabrina K Silva; ALVARENGA, LARISSA; NGUYEN, C; ISABELLA GIZZI JIACOMINI; Fanny Boursin; Arnaud Leroy; Magali Noiray; Juliana Ferreira de Moura; Nicolas Aubrey; Philippe BILLIALD
Toxins, v. 12, p. 256 2020 DOI
Palavra-chave: Venom; antivenom; neutralization; Loxosceles; sphingomielinases D; humanization
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Envenoming due to Loxosceles spider bites still remains a neglected disease of particular medical concern in the Americas. To date, there is no consensus for the treatment of envenomed patients, yet horse polyclonal antivenoms are usually infused to patients with identified severe medical conditions. It is widely known that venom proteins in the 30–35 kDa range with sphingomyelinase D (SMasesD) activity, reproduce most of the toxic effects observed in loxoscelism. Hence, we believe that monoclonal antibody fragments targeting such toxins might pose an alternative safe and effective treatment. In the present study, starting from the monoclonal antibody LimAb7, previously shown to target SMasesD from the venom of L. intermedia and neutralize its dermonecrotic activity, we designed humanized antibody V-domains, then produced and purified as recombinant single-chain antibody fragments (scFvs). These molecules were characterized in terms of humanness, structural stability, antigen-binding activity, and venom-neutralizing potential. Throughout this process, we identified some blocking points that can impact the Abs antigen-binding activity and neutralizing capacity. In silico analysis of the antigen/antibody amino acid interactions also contributed to a better understanding of the antibody’s neutralization mechanism and led to reformatting the humanized antibody fragment which, ultimately, recovered the functional characteristics for efficient in vitro venom neutralization.
DE SOUZA LEANDRO, ANDRÉ; DIB GONÇALVES, DANIELA; Mário Antonio NavarroSilva; MARIA PALACIO'CORTÈS, ANGELA; SCHUARTZ, VALÉRIA; CRISTIANE SIBIM, ALESSANDRA; ANTONIO CHIBA DE CASTRO, WAGNER; AVEMIR RIOS, JEAN; DA SILVA BRITTO, ANDRÉ; ROBERTO GALVÃO, SANDRO; DEFANTE LOPES, RENATA; VELEH RIVAS, AÇUCENA; AMARAL MARTINS, CAROLINE; DA SILVA, ISAAC; MICHAEL DELAI, ROBSON
TROPICAL MEDICINE & INTERNATIONAL HEALTH, v. 1, n. 0, p. 00-00, 2020 DOI
Palavra-chave: Diptera; Culicidae; Aedes aegypti; controle de mosquitos
Áreas do conhecimento: Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia; Ciências Biológicas; Zoologia; Zoologia Aplicada; Controle Populacional de Animais
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Objective In Brazil, the most common method of controlling outbreaks of arbovirus is by the use of chemical sprays, which kill the insect vector, Aedes aegypti. The main objective of this study was ...
MACHADO, TATIANE WINKLER MARQUES; RODRIGUES, JENIFER MOTA; MORO, TATIANA ROJO; DUARTE, MARIA EUGÊNIA RABELLO; Miguel Noseda
Applied Sciences-Basel, v. 10, n. 7, p. 2239 2020 DOI
Palavra-chave: Microalgae; Pavlova; polysaccharides; B-glucans
Áreas do conhecimento: Ciências Biológicas; Biotecnologia; Ciências Biológicas; Bioquímica; Bioquímica
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Different molecules have been tested as analog receptors due to their capacity to bind bacteria and prevent cell adhesion. By using in vitro assays, the present study characterized the aqueous and alkaline extracts from microalgae Pavlova lutheri and Pavlova gyrans and evaluated the capacity of these extracts to adhere to enterobacteria (Salmonella Typhimurium). The aqueous and alkaline extracts of both species were fractionated via freeze-thawing, giving rise to soluble and insoluble (precipitate) fractions in cold water. The obtained fractions were studied using thermogravimetric, methylation analyses, and using 1D and 2D NMR techniques. The cold-water-soluble fractions obtained from the aqueous extracts were mainly composed of highly branched (1→3),(1→6)-β-glucans, whereas the cold-water-precipitate fractions were constituted by (1→3)-β-glucans. The alkaline extract fractions showed similar compositions with a high protein content, and the presence of glycosides (sulfoquinovosylglycerol (SQG), digalactosylglycerol (DGG)), and free fatty acids. The linear (1→3)-β-glucans and the alkaline extract fractions showed an adhesion capacity toward Salmonella. The chemical composition of the active fractions suggested that the presence of three-linked β-glucose units, as well as microalgal proteins and glycosides, could be important in the adhesion process. Therefore, these microalgal species possess a high potential to serve as a source of anti-adhesive compounds.
Valéria Bumiller Bini; Camila Freitas Oliveira-Toré; Tamyres Mingorance Carvalho; Gabriela Canalli Kretzschmar; Letícia Boslooper Gonçalves; Nina de Moura Alencar; Miguel Ângelo Gasparetto Filho; Márcia Holsbach Beltrame; Angelica Beate Winter Boldt
GENETICS AND MOLECULAR BIOLOGY (ONLINE VERSION), v. 44, n. 1, p. e20200199 2020 DOI
Palavra-chave: MASP1; masp2; COVID-19; SARS-CoV-2; coagulação; complemento
Áreas do conhecimento: Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia; Ciências Biológicas; Genética; Genética Humana e Médica; Imunogenética; Ciências Biológicas; Genética; Genética Humana e Médica; Imunogenética
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Abstract Components of the complement system and atypical parameters of coagulation were...
Janaína Marques de Almeida; Robson Carlos Alnoch; Emanuel Maltempi de Souza; MITCHELL, DAVID ALEXANDER; KRIEGER, NADIA
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1868, p. 140320 2020 DOI
Palavra-chave: lipase; metagenomics; biocatalysis
Áreas do conhecimento: Engenharias; Engenharia Química; Processos Industriais de Engenharia Química; Processos Bioquímicos; Ciências Biológicas; Bioquímica; Enzimologia; Ciências Biológicas; Biotecnologia; Biotecnologia Industrial
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