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Publicações - ciências biológicas

Biochemical characterization and application of a new lipase and its cognate foldase obtained from a metagenomic library derived from fat-contaminated soil Janaína Marques de Almeida; Viviane Paula Martini; Jorge Iulek; Robson Carlos Alnoch; Vivian Rotuno Moure; Marcelo Müller dos Santos; Emanuel Maltempi de Souza; MITCHELL, DAVID ALEXANDER; KRIEGER, NADIA INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, p. 442-454, 2019 DOI Palavra-chave: lipases; metagenômica; foldase; imobilização; Resolução de misturas racêmicas Áreas do conhecimento: Ciências Biológicas; Bioquímica dos Microorganismos; Biotecnologia resumo ...
Biochemical characterization and application of a new lipase and its cognate foldase obtained from a metagenomic library derived from fat-contaminated soil ALMEIDA, JANAINA MARQUES; Viviane P Martini; Jorge Iulek; ALNOCH, ROBSON CARLOS; Vivian Rotuno Moure; Marcelo Muller dos Santos; Souza, Emanuel Maltempi; MITCHELL, DAVID ALEXANDER; KRIEGER, NADIA INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v. 137, p. 442-454, 2019 DOI Home page Palavra-chave: metagenômica; Biocatálise; lipase Áreas do conhecimento: Ciências Biológicas; Enzimologia resumo ... LipMF3 is a new lipase isolated from a metagenomic library derived from a fat-contaminated soil. It belongs to the lipase subfamily I.1 and has identi…
Biological, biochemical and genotoxic effects of Sb in the midge Chironomus sancticaroli Strixino and Strixino, 1981 (Diptera: Chironomidae) Gisele dos Santos Morais; VIEIRA, THALITA BASTIDA; SANTOS, GUSTAVO SOUZA; BAIKA, LOANA M.; Cestari, M.M.; GRASSI, MARCO TADEU; NAVARRO DA SILVA, MÁRIO ANTÔNIO ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 176, p. 196-203, 2019 DOI Palavra-chave: ensaio cometa; Estresse Oxidativo; Genotoxicidade Áreas do conhecimento: Ciências Biológicas; Genética; genotoxicidade; Ciências Biológicas; Genética; Toxicidade resumo ...
Biological, biochemical and genotoxic effects of Sb in the midge Chironomus sancticaroli Strixino and Strixino, 1981 (Diptera: Chironomidae) Gisele dos Santos Morais; VIEIRA, THALITA BASTIDA; SANTOS, GUSTAVO SOUZA; BAIKA, LOANA M.; Cestari, M.M.; GRASSI, MARCO TADEU; Mário Antonio NavarroSilva ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 176, p. 196-203, 2019 DOI Palavra-chave: Diptera; Chironomidae; Chironomus sancticaroli; Enzimas Antioxidante Áreas do conhecimento: Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia; Ciências Biológicas; Zoologia; Fisiologia dos Grupos Recentes resumo ...
Biomass production and harvesting of Desmodesmus subspicatus cultivated in flat plate photobioreactor using chitosan as flocculant agent CORRÊA, DIEGO DE OLIVEIRA; DUARTE, MARIA EUGÊNIA RABELLO; Miguel Noseda JOURNAL OF APPLIED PHYCOLOGY, v. 31, n. 2, p. 857-866, 2019 DOI Palavra-chave: Microalgae; biomass production; photobioreactor; Chitosan; Flocculant agent; Desmodesmus subspicatus Áreas do conhecimento: Ciências Biológicas; Biotecnologia; Ciências Biológicas; Biotecnologia; Biotecnologia Ambiental e Recursos Naturais; Bioprospecção resumo ... Microalgal biomass production has been investigated since the 1950s by academic and industry sectors due to its potential and for biodiesel production. One of the main bottlenecks in microalgae production is biomass recovery and its separation from aqueous medium. For this reason, the use of a flocculation process is an important step to promote microalgae recovery in large scale. Here we provide a strategy for the harvesting of microalgal biomass using chitosan as flocculating agent in pilot scale cultures performed in flat plate photobioreactor. The results show that chitosan was effective in inducing microalgae flocs and separating them by settling. In the jar test scale the best clarification efficiency was around 99% and when scaled up using a 100-L photobioreactor, the biomass recovery efficiency was close to 98%. Comparison of the compositions of biomasses obtained by flocculation and centrifugation indicates no significant differences in terms of carbohydrates, proteins, lipids, and ashes, showing that the flocculation process is not affecting the biomass characteristics and its potential biotechnological applications. Based on the presented results, flocculation using chitosan as flocculant agent can be considered as an efficient method to harvest Desmodesmus subspicatus biomass cultured in pilot scale photobioreactors.
Bioprospection of proteases from Halobacillus andaensis for bioactive peptide production from fish muscle protein DELGADO-GARCÍA, MARIANA; FLORES-GALLEGOS, ADRIANA C.; KIRCHMAYR, MANUEL; RODRÍGUEZ-GONZÁLEZ, JORGE; MATEOS-DÍAZ, JUAN C.; AGUILAR, CRISTOBAL N.; Marcelo Muller dos Santos; CAMACHO-RUÍZ, ROSA M. ELECTRONIC JOURNAL OF BIOTECHNOLOGY, v. 39, p. 52-60, 2019 DOI Palavra-chave: Arquea Halofílica; Biocatálise Áreas do conhecimento: Ciências Biológicas; Microbiologia Aplicada resumo ...
Brown Spider (Loxosceles) Venom Toxins as Potential Biotools for the Development of Novel Therapeutics Daniele Chaves-Moreira; OLGA CHAIM; Arni, Raghuvir Krishnaswamy; Silvio Sanches Veiga; Fernando Hitomi Matsubara; Zelinda SCHEMCZSSEN; Elidiana de Bona; Vanessa Ribeiro Heidemann; Clara Guerra Duarte; Luiza Helena Gremski; Carlos Chávez-Olórtegui; Andrea Senff Ribeiro Toxins, v. 11, n. 6, p. 355 2019 DOI Palavra-chave: Aranha marrom; Veneno; Toxinas Áreas do conhecimento: Ciências Biológicas; Morfologia; Citologia e Biologia Celular; Ciências Biológicas; Bioquímica; Biologia Molecular resumo ... Brown spider envenomation results in dermonecrosis with gravitational spreading characterized by a marked inflammatory reaction and with lower prevalence of systemic manifestations such as renal failure and hematological disturbances. Several toxins make up the venom of these species, and they are mainly peptides and proteins ranging from 5–40 kDa. The venoms have three major families of toxins: phospholipases-D, astacin-like metalloproteases, and the inhibitor cystine knot (ICK) peptides. Serine proteases, serpins, hyaluronidases, venom allergens, and a translationally controlled tumor protein (TCTP) are also present. Toxins hold essential biological properties that enable interactions with a range of distinct molecular targets. Therefore, the application of toxins as research tools and clinical products motivates repurposing their uses of interest. This review aims to discuss possibilities for brown spider venom toxins as putative models for designing molecules likely for therapeutics based on the status quo of brown spider venoms. Herein, we explore new possibilities for the venom components in the context of their biochemical and biological features, likewise their cellular targets, three-dimensional structures, and mechanisms of action.
Cage aquaculture in Neotropical waters promotes attraction and aggregation of fish PEREIRA, LARISSA STRICTAR; DEMÉTRIO, JOSÉ ANTÔNIO; Almir Manoel Cunico; João Dirço Latini; Luiz Carlos Gomes; Angelo Antonio Agostinho AQUACULTURE RESEARCH (ONLINE), v. 50, n. 10, p. 2896-2906, 2019 DOI Palavra-chave: aquaculture impact; conservation; fish‐farming; reservoir; Tilápia resumo ...
Cannabidiol attenuates mechanical allodynia in streptozotocin-induced diabetic rats via serotonergic system activation through 5-HT1A receptors JESUS, CARLOS HENRIQUE ALVES; Janaina Menezes Zanoveli; Cunha, Joice M.; REDIVO, DAIANY DARLLY BELLO; GASPARIN, ALEXIA THAMARA; SOTOMAIOR, BRUNA BITTENCOURT; DE CARVALHO, MILENE CRISTINA; GENARO, KARINA; ZUARDI, ANTONIO WALDO; HALLAK, JAIME EDUARDO CECÍLIO; CRIPPA, JOSÉ ALEXANDRE BRAIN RESEARCH, v. 1715, p. 156-164, 2019 DOI Palavra-chave: canabidiol; estreptozotocina; dor neuropática Áreas do conhecimento: Ciências Biológicas; Farmacologia da Dor; Hiperalgesia Inflamatória resumo ...
Cannabinoid CB1 receptor antagonist rimonabant decreases levels of markers of organ dysfunction and alters vascular reactivity in aortic vessels in late sepsis in rats LEITE-AVALCA, MARIANE C. G.; Fernanda Staats; Dunia Verona; de Souza, Priscila; Maria Camila de Almeida; José Eduardo da Silva Santos; Aleksander Roberto Zampronio Inflammation, v. 42, p. 618-627, 2019 DOI Palavra-chave: sepse; endocanabinóides Áreas do conhecimento: Ciências Biológicas; Farmacologia da Inflamação resumo ... Sepsis is a life-threatening condition with high mortality rates that is caused by dysregulation of the host response to infection. We previously showed that treatment with the cannabinoid CB1 receptor antagonist rimonabant reduced mortality rates in animals with sepsis that was induced by cecal ligation and puncture (CLP). This improvement in the survival rate appeared to be related to an increase in arginine vasopressin (AVP) levels 12 h after CLP. The present study investigated the effects of rimonabant on organ dysfunction, hematologic parameters, and vascular reactivity in male Wistar rats with sepsis induced by CLP. Intraperitoneal treatment with rimonabant (10 mg/kg, 4 h after CLP) abolished the increase in the plasma levels of lactate, lactate dehydrogenase, glucose, and creatinine kinase MB without altering hematological parameters (i.e., leukopenia and a reduction of platelet counts). CLP increased plasma levels of nitrate/nitrite (NOx) and induced vasoconstriction in the tail artery. The treatment of CLP rats with rimonabant did not alter NOx production but reduced the vasoconstriction. Rimonabant also attenuated the hyperreactivity to AVP induced by CLP without affecting hyporesponsiveness to phenylephrine in aortic rings. These results suggest that rimonabant reduces organ dysfunction during sepsis, and this effect may be related to AVP signaling in blood vessels. This effect may have contributed to the higher survival rate in rimonabant-treated septic animals.

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