Publicações - ciências biológicas


Loraine Beatriz Acosta Veiga; RIBEIRO, ENILZE M.S.F.; Virgínia Maria Cóser; Luciane R. Cavalli; CAVALLI, IGLENIR J.; Rodrigues, JN.; Waldir Veiga Pereira; D V Pereira; Lafayette,TCS; Bassem R Haddad
Leukemia Res., v. 28, p. 1033-1038, 2004
Palavra-chave: LLA; t(12;21)(p13;q22); fish
Áreas do conhecimento: Ciências Biológicas; Genética Humana e Médica
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Marcelo Pilonetto; Edvaldo Antonio Ribeiro Rosa; Paulo R Brofman; Daniela Baggio; Francine Calvário; Cristiano Schelp; Aguinaldo José do Nascimento; Lara Messias Reason
The Brazilian Journal of Infectious Diseases, v. 8, p. 206-210, 2004 Home page
Palavra-chave: Gown; Bacteria; hospital infection; cross contamination
Áreas do conhecimento: Ciências Biológicas; Microbiologia Aplicada; Microbiologia Médica
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M L M Fernandes; KRIEGER, NADIA; A M Baron; Patricio Guillermo Peralta-Zamora; Luiz Pereira Ramos; MITCHELL, DAVID ALEXANDER
Journal of Molecular Catalysis. B, Enzymatic (Print), v. 30, p. 43-49, 2004 DOI
Palavra-chave: lipases; Biocatálise; Micelas Invertidas
Áreas do conhecimento: Ciências Biológicas; Enzimologia; Biotecnologia; Ciências Biológicas; Bioquímica; Enzimologia
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Cynton Lourenço Corrêa; Silva, Simone Florim; Lowe, Jennifer; TORTELOTE, G; Einicker-Lamas, Marcelo; Martinez, Ana M. Blanco; ALLODI, S
Cell and Tissue Research (Print), v. 318, p. 609-615, 2004 DOI
Palavra-chave: Crustáceo; sistema visual; ultraestrutura
Áreas do conhecimento: Ciências Biológicas; Fisiologia; Neurociências
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Neurofilaments (NFs) have not been observed in crustaceans using conventional electron microscopy, and intermediate filaments have never been described in crustaceans and other arthropods by immunocytochemistry. Since polypeptides, labeled by the NN18-clone antibody, were revealed on microtubule side-arms of crayfish, we have tested, in this study, whether proteins similar to mammalian NFs are present in the protocerebral tract (PCT) of the crab Ucides cordatus. We used immunohistochemistry for light microscopy with monoclonal antibodies against three different NF subunits, high (NF-H), medium (NF-M), and light (NF-L). Labeling was observed with the NN18-clone, which recognizes NF-M. In order to confirm the results obtained with the immunohistochemical reactions, Western blotting, using the three primary antibodies, was performed and the presence of NF-M was confirmed. The NN18-clone monoclonal antibody recognized a protein of ≈160 kDa, similar to the mammaliam NF-M protein, but NF-L and NF-H were not recognized. Conventional transmission electron microscopy was used to observe the ultrastructural components of the axons and immunoelectron microscopy was used to show the distribution of the NF-M-like polypeptides along cytoskeletal elements of the PCT. Our results agree with previous studies on crustacean NF proteins that have reported negative immunoreactions against NF-H and NF-L subunits and positive immunoreactions against the mammalian NF-M subunit. However, the protein previously referred to as P600 and recognized by the NN18-clone, has a very high molecular weight, thus, being different from mammalian NF-M subunit and from the protein revealed now in our study.
André Bellin Mariano; Caroline Valente; Leonardo Kovalhuk; Juliana Maurer-Menestrina; Adaucto Bellarmino Pereira-Netto; Miguel Pedro Guerra; E G Carnieri
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, v. 47, n. 6, p. 873-879, 2004 DOI Home page
Palavra-chave: Araucaria angustifolia; Mitocondria vegetal; consumo de oxigênio
Áreas do conhecimento: Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia
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A method for the isolation of coupled mitochondria from the callus of Araucaria angustifolia is...
André Bellin Mariano; Leonardo Kovalhuk; Caroline Valente; Juliana Maurer Menestrina; Adaucto Bellarmino Pereira-Neto; Miguel Pedro Guerra; Eva Gunilla Skare Carnieri
Brazilian Archives of Biology and Technology, v. 47, p. 873-879, 2004
Palavra-chave: Araucaria angustifolia; plant mitochondria; respiratory chain; callus
Áreas do conhecimento: Ciências Biológicas; Bioquímica; Metabolismo e Bioenergética; Metabolismo Hepático; Ciências Biológicas; Botânica; Fisiologia Vegetal
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André Bellin Mariano; Adaucto Bellarmino de Pereira Netto; Leonardo Kovalhuk; Caroline Valente; J. M. MENESTRINA; Miguel Pedro Guerra; Eva Gunilla Skare Carnieri
Brazilian Archives of Biology and Technology (Impresso), v. 47, n. 6, p. 873-879, 2004 DOI Home page
Áreas do conhecimento: Ciências Biológicas; Fisiologia Vegetal; Biotecnologia Vegetal; Ciências Biológicas; Botânica; Fisiologia Vegetal
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A method for the isolation of coupled mitochondria from the callus of Araucaria angustifolia is...
Mariana S. Araujo; Valter A. Baura; Emanuel Maltempi de Souza; Elaine Machado Benelli; STEFFENS, M; Leda Satie Chubatsu; Liu Un Rigo
Protein Expression and Purification, v. 33, p. 19-24, 2004
Palavra-chave: Azospirillum Brasilense, Fixacao de Nitrogenio; GlnZ Protein; Expression and purification
Áreas do conhecimento: Ciências Biológicas; Bioquímica; Bioquímica
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Mariana S Araújo; Valter A. Baura; Emanuel Maltempi de Souza; Elaine Machado Benelli; RIGO, L; STEFFENS, M; Fábio de Oliveira Pedrosa; Leda Satie Chubatsu
Protein Expression and Purification, v. 204, p. 19-24, 2004
Palavra-chave: Azospirillum; GlnZ; Protein purification
Áreas do conhecimento: Ciências Biológicas; Biologia Molecular; Ciências Biológicas; Bioquímica; Bioquímica dos Microorganismos
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Mariana S Araújo; Valter A. Baura; Emanuel Maltempi de Souza; Elaine Machado Benelli; RIGO, L; STEFFENS, M; Fábio de Oliveira Pedrosa; Leda Satie Chubatsu
Protein Expression and Purification, v. 33, p. 19-24, 2004 DOI
Áreas do conhecimento: Ciências Biológicas; Biologia Molecular
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