Publicações - ciências biológicas
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ROCHA, GISELI SWERTS;
Ana Teresa Lombardi;
ESPÍNDOLA, EVALDO L.G.
Palavra-chave:
ecotoxicidade
Áreas do conhecimento:
Ciências Biológicas; ECOTOXICOLOGIA
resumo ...
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BRAZÃO, CLAUDIA CARAMELO;
KRACIZY, RAFAEL ORTIZ;
DUTRA, FABRÍCIO MARTINS;
DE OLIVEIRA, ANA PAULA;
Rafael Itamar;
Eduardo Luis Cupertino Ballester
Palavra-chave:
nitrogenados;
Toxicidade
Áreas do conhecimento:
CIENCIAS_AGRARIAS; Aqüicultura;
Ciências Biológicas; Biologia Geral; Aquacultura; Carcinicultura
resumo ...
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DZIEDZIC, DILCELE SILVA MOREIRA;
DE CARVALHO, KATHERINE ATHAYDE TEIXEIRA;
FRANCISCO, JULIO CESAR;
MOGHARBEL, BASSAM FELIPE;
IRIODA, ANA CAROLINA;
STRICKER, PRISCILA ELIAS FERREIRA;
FLORIANO, JULIANA;
DE NORONHA, LÚCIA;
ABDELWAHID, ELTYEB;
Franco, Celia R.C.
Palavra-chave:
Biomaterials;
Amniotic Membrane;
Adipose Tissue;
Promoter of Calcification in Bone Regeneration
Áreas do conhecimento:
Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia
resumo ...
Discarded tissues, like human amniotic membranes and adipose tissue, were investigated for the application of Decellularized Human Amniotic Membrane (DAM) as a viable scaffold for transplantation of Adipose-derived stromal cells (ASCs) in bone regeneration of non-healing calvarial defects in rats. Amniotic membrane was decellularized to provide a scaffold for male Wistar rats ASCs expansion and transplantation. ASCs osteoinduction in vitro promoted the deposition of a mineralized bone-like matrix by ASCs, as calcified globular accretions associated with the cells on the DAM surface and inside the collagenous matrix. Non-healing calvarial defects on male Wistar rats were randomly divided in control without treatment, treatment with four layers of DAM, or four layers of DAM associated with ASCs. After 12 weeks, tissue blocks were examined by micro-computed tomography and histology. DAM promoted osteoconduction by increasing the collagenous matrix on both DAM treatments. DAM with ASCs stimulated bone deposition, demonstrated by a higher percentage of bone volume and trabecular bone number, compared to control. Besides the osteogenic capacity in vitro, ASCs stimulated the healing of calvarial defects with significant DAM graft incorporation concomitant with higher host bone deposition. The enhanced in vivo bone regeneration by undifferentiated ASCs loaded onto DAM confirmed the potential of an easily collected autologous cell source associated with a broadly available collagenous matrix in tissue engineering.
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VELUZA, DANILA SYRIANI;
RECHETELO, JULIANA;
Emygdio Leite de Araújo Monteiro-Filho;
MESTRE, LUIZ AUGUSTO MACEDO
Palavra-chave:
aves;
manguezal;
forrageamento;
Pelecaniforme
Áreas do conhecimento:
Ciências Biológicas; Comportamento Animal;
Ciências Biológicas; Zoologia; Zoologia;
Ciências Biológicas; Ecologia; Ecologia Aplicada
resumo ...
(pt)
A eficiência de forrageio das aves, resultado do balanço entre consumo e gasto de energia, pode variar conforme condições locais. A eficiência de forrageio foi comparada entre Egretta thula (adulto N = 11) e Egretta caerulea (adulto N = 62 e jovem N = 17) no estuário da cidade de Paranaguá, Paraná, Brasil. Em 14 h de observações divididas em sessões de 2 minutos por indivíduo, foram registrados locais de forrageio (lodo ou águas rasas), número de passos, bicadas totais e bicadas bem-sucedidas, e calculadas taxas de sucesso, esforço energético e eficiência de captura. Em águas rasas E. thula foi mais eficiente que E. caerulea . Não houve diferença para E. thula entre os ambientes, enquanto adultos de E. caerulea foram mais eficientes que os jovens, com maior eficiência no lodo do que em águas rasas. Diferenças na eficiência de forrageio entre as espécies podem ter relação com o tipo de forrageio entre elas dependendo da localidade. COMPARISON OF EGRETTA CAERULEA AND EGRETTA THULA FORAGING EFFICIENCY IN ESTUARY OF OF PARANAGUÁ, PARANÁ, SOUTHERN BRAZIL : The foraging efficiency of birds may vary according to local conditions as a result of the balance between energy consumption and expenditure. The foraging efficiency of Egretta thula (adults n=11) and Egretta caerulea (adults n=69 and juveniles n= 17) was compared in Paranaguá city estuary, in Paraná, Brazil. In 14 hours of observation divided into 2 minutes sessions per individual, were recorded foraging site (mud or shallow water), number of steps, total peckings and successful peckings, and were calculated the success rates, energy effort and capture efficiency. In shallow water E.thula was more efficient than E. caerulea . There was no intraspecific differences in E. thula regarding environments. Adults of E. caerulea were more efficient than juveniles, with greater efficiency in mud than in shallow waters environments. Differences in foraging efficiency between species may be related to the type of foraging according to feeding location.
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Danila Syriani Veluza;
Juliana Rechetelo;
Emygdio L. A. Monteiro-Filho;
Luiz Augusto Macedo Mestre
Palavra-chave:
aves;
forrageamento;
manguezal;
Pelecaniformes
resumo ...
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Carolina Amaral Bueno Azevedo;
Regiane Stafim da Cunha;
Carolina Victoria Cruz Junho;
Jessica Verônica da Silva;
Andréa Moreno-Amaral;
Thyago Proença de Moraes;
CARNEIRO-RAMOS, MARCELA SORELLI;
Andréa Emília Marques Stinghen
Palavra-chave:
Uremic Toxins;
peritoneal dialysis;
extracellular vesicles
Áreas do conhecimento:
Ciências da Saúde; nephrology;
Ciências da Saúde; Medicina; Nefrologia Experimental;
Ciências Biológicas; Bioquímica; Bioquímica
resumo ...
Uremic toxins (UTs) accumulate in the circulation of patients with chronic kidney disease (CKD). High volume hemodiafiltration (HDF) improves clearanc…
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BERTI, FERNANDA COSTA BRANDÃO;
MATHIAS, CAROLINA;
GARCIA, LEANDRO E.;
Daniela Fiori Gradia;
DE ARAÚJO SOUZA, PATRÍCIA SAVIO;
CIPOLLA, GABRIEL ADELMAN;
DE OLIVEIRA, JAQUELINE CARVALHO;
MALHEIROS, DANIELLE
Palavra-chave:
competing endogenous;
Xist;
HPV16;
HPV18;
cervical cancer
Áreas do conhecimento:
Ciências Biológicas; Genética Humana e Médica; Genética Geral;
Ciências Biológicas; Genética; Genética Humana e Médica; Imunogenética
resumo ...
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Lorenzoni, Paulo José;
Scola, Rosana Herminia;
DUCCI, RENATA DAL-PRÁ;
ARNDT, RAQUEL CRISTINA;
HRYSAY, NYVIA MILICIO COBLINSKI;
FUSTES, OTTO JESUS HERNANDEZ;
TÖPF, ANA;
LOCHMÜLLER, HANNS;
Lineu Cesar Werneck;
CLAUDIA S K KAY
Palavra-chave:
miastenia congênita
Áreas do conhecimento:
Ciências Biológicas; Genética Humana e Médica; Neurologia
resumo ...
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Fernanda Kashiwagi;
Brenno Wendler Miranda;
Fábio de Oliveira Pedrosa;
Emanuel Maltempi de Souza;
Marcelo Muller dos Santos
Palavra-chave:
Interação Planta-Microrganismo;
Biologia Sintética
Áreas do conhecimento:
Ciências Biológicas; Biologia Sintética
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Control of gene expression is crucial for several biotechnological applications, especially for implementing predictable and controllable genetic circuits. Such circuits are often implemented with a transcriptional regulator activated by a specific signal. These regulators should work independently of the host machinery, with low gratuitous induction or crosstalk with host components. Moreover, the signal should also be orthogonal, recognized only by the regulator with minimal interference with the host operation. In this context, transcriptional regulators activated by plant metabolites as flavonoids emerge as candidates to control gene expression in bacteria. However, engineering novel circuits requires the characterization of the genetic parts (e.g., genes, promoters, ribosome binding sites, and terminators) in the host of interest. Therefore, we decomposed the QdoR regulatory system of B. subtilis, responsive to the flavonoid quercetin, and reassembled its parts into genetic circuits programmed to have different levels of gene expression and noise dependent on the concentration of quercetin. We showed that only one of the promoters regulated by QdoR worked well in E. coli, enabling the construction of other circuits induced by quercetin. The QdoR expression was modulated with constitutive promoters of different transcriptional strengths, leading to low expression levels when QdoR was highly expressed and vice versa. E. coli strains expressing high and low levels of Qdo...
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KOCH, GABRIEL;
LUCIMERIS RUARO SCHUTA;
CALEGARIO, RENATA FAIER;
João Carlos Bespalhok Filho;
DAROS, EDELCLAITON;
Ricardo Augusto de Oliveira;
DUARTE, HENRIQUE DA SILVA SILVEIRA
Palavra-chave:
Sugarcane breeding;
ferrugem;
seleção;
ferrugem alaranjada;
ferrugem marrom
Áreas do conhecimento:
Ciências Biológicas; Biotecnologia; Biotecnologia Vegetal; Melhoramento de Plantas
resumo ...
Orange rust (OR) and brown rust (BR) are among the most important diseases of sugarcane. The objective of this work was to evaluate the efficiency of fungicides in the control of both rusts. Two experiments, one for each rust, were conducted in the state of Paraná, Brazil, in plant cane and in ratoon. A randomized block design with 8 treatments and three replications was used. The treatments were: control (T1), pyraclostrobin (T2), pyraclostrobin + epoxiconazole (T3), azoxystrobin + cyproconazole (T4), azoxystrobin + tebuconazole (T5), trifloxystrobin + tebuconazole (T6), azoxystrobin + benzovindiflupyr (T7) and pyraclostrobin + fluxapyroxad (T8). The cultivars RB72454 and RB835486 were used for their susceptibility to OR and BR, respectively. Four applications of fungicides were carried out in plant cane and also in ratoon, in a 30-day intervals. With the severity data over time, the relative area under the disease progress curve (rAUDPC) was calculated. After harvest, yield (tons of cane per hectare-TCH), yield recovery compared to the control (YR) (%), pol% cane (PC) and tons of pol per hectare (TPH) were obtained. From rAUDPC results, treatments T3, T4, T5, and T7 were the most efficient for the control of OR and treatments T2, T3, T4, T5, T7 and T8 were the most efficient for controlling BR. Yield was affected by treatments only in ratoon in both experiments. The YR ranged from 18.5% to 31.1% and from 16.1% to 37.7% to OR and BR, respectively. There were no differences between treatments for PC in both experiments and TPH was influenced by treatments in ratoon in the BR experiment.
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