Publicações - ciências biológicas
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KOCH, GABRIEL;
LUCIMERIS RUARO SCHUTA;
CALEGARIO, RENATA FAIER;
João Carlos Bespalhok Filho;
DAROS, EDELCLAITON;
Ricardo Augusto de Oliveira;
DUARTE, HENRIQUE DA SILVA SILVEIRA
Palavra-chave:
Sugarcane breeding;
ferrugem;
seleção;
ferrugem alaranjada;
ferrugem marrom
Áreas do conhecimento:
Ciências Biológicas; Biotecnologia; Biotecnologia Vegetal; Melhoramento de Plantas
resumo ...
Orange rust (OR) and brown rust (BR) are among the most important diseases of sugarcane. The objective of this work was to evaluate the efficiency of fungicides in the control of both rusts. Two experiments, one for each rust, were conducted in the state of Paraná, Brazil, in plant cane and in ratoon. A randomized block design with 8 treatments and three replications was used. The treatments were: control (T1), pyraclostrobin (T2), pyraclostrobin + epoxiconazole (T3), azoxystrobin + cyproconazole (T4), azoxystrobin + tebuconazole (T5), trifloxystrobin + tebuconazole (T6), azoxystrobin + benzovindiflupyr (T7) and pyraclostrobin + fluxapyroxad (T8). The cultivars RB72454 and RB835486 were used for their susceptibility to OR and BR, respectively. Four applications of fungicides were carried out in plant cane and also in ratoon, in a 30-day intervals. With the severity data over time, the relative area under the disease progress curve (rAUDPC) was calculated. After harvest, yield (tons of cane per hectare-TCH), yield recovery compared to the control (YR) (%), pol% cane (PC) and tons of pol per hectare (TPH) were obtained. From rAUDPC results, treatments T3, T4, T5, and T7 were the most efficient for the control of OR and treatments T2, T3, T4, T5, T7 and T8 were the most efficient for controlling BR. Yield was affected by treatments only in ratoon in both experiments. The YR ranged from 18.5% to 31.1% and from 16.1% to 37.7% to OR and BR, respectively. There were no differences between treatments for PC in both experiments and TPH was influenced by treatments in ratoon in the BR experiment.
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C A Oliveira Ribeiro;
Azevedo, Juliana S.;
Robie Alan Bombardelli;
Marco A F Randi
Palavra-chave:
MXR;
Rhamdia quelen;
Cobre Inorganico;
biomarcadores;
Estagio Inicial de desenvolvimento
Áreas do conhecimento:
Ciências Biológicas; Ecologia Aplicada; Ecotoxicologia
resumo ...
(en)
P-glycoproteins (P-gp) and Multidrug resistance protein (MRP) represent a family of ABC (ATP-binding cassette) transporters responsible for multixenobiotic resistance mechanism (MXR) in aquatic organisms. In the current study the modulation of P-gp and MRP proteins was evaluated in embryo and larvae of Rhamdia quelen fish species exposed to copper. Adult females were exposed by gavage during 60 days to copper (5 mg Cu kg-1) and eggs, embryos, and larvae from exposed and unexposed females were exposed to 30 mg Cu L-1. The activity of ABC transporters was accessed via calcein accumulation assay using the specific inhibitors: Verapamil (P-gp) and MK571 (MRP). P-gp activity was detected in all analyzed stages whereas MRP activity was observed after 36 and 96 hpf. Oocytes from females previously exposed and larvae stages (36 and 96 hpf) accumulated less calcein than no exposed oocytes, showing higher ABC transporters activity. In individuals exposed to copper, a higher inhibitory effect was observed 1 hpf. The modulation of ABC transporter proteins is time dependent throughout the development, and the initial stages are more sensible to copper. These findings highlight the MXR mechanism as a biomarker of pollutant exposure in early stages of development of R. quelen.
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C.A., OLIVEIRA RIBEIRO;
A.C.B., AZEVEDO;
R.A., BOMBARDELLI;
M. A. F. Randi
Palavra-chave:
mecanismo MXR;
Rhamdia quelem;
biomarcadores;
Cobre Inorganico;
estágios iniciais do desenvolvimento
Áreas do conhecimento:
Ciências Biológicas; Ecologia; Ecologia Aplicada
resumo ...
(en)
P-glycoproteins (P-gp) and Multidrug resistance protein (MRP) represent a family of ABC (ATP-binding cassette) transporters responsible for multixenobiotic resistance mechanism (MXR) in aquatic organisms. In the current study the modulation of P-gp and MRP proteins was evaluated in embryo and larvae of Rhamdia quelen fish species exposed to copper. Adult females were exposed by gavage during 60 days to copper (5 mg Cu kg-1) and eggs, embryos, and larvae from exposed and unexposed females were exposed to 30 mg Cu L-1. The activity of ABC transporters was accessed via calcein accumulation assay using the specific inhibitors: Verapamil (P-gp) and MK571 (MRP). P-gp activity was detected in all analyzed stages whereas MRP activity was observed after 36 and 96 hpf. Oocytes from females previously exposed and larvae stages (36 and 96 hpf) accumulated less calcein than no exposed oocytes, showing higher ABC transporters activity. In individuals exposed to copper, a higher inhibitory effect was observed 1 hpf. The modulation of ABC transporter proteins is time dependent throughout the development, and the initial stages are more sensible to copper. These findings highlight the MXR mechanism as a biomarker of pollutant exposure in early stages of development of R. quelen.
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FIGUEIREDO, DAVID LIVINGSTONE ALVES;
RAMÃO, ANELISA;
Boldt, Angelica Beate Winter;
MOYA, CARLA FREDRICHSEN;
CHIN, CHUNG MAN;
PAULA, DANIEL DE;
RECH, DANIEL;
GRADIA, DANIELA FIORI;
Danielle Malheiros Ferreira;
VENTURINI, DANIELLE;
TAVARES, ELIANDRO REIS;
XIMENEZ, JOÃO PAULO BIANCHI;
SEIVA, FÁBIO RODRIGUES FERREIRA;
PANIS, CAROLINA;
BEZERRA, RAFAEL DOS SANTOS;
FERRASA, ADRIANO;
Petzl-Erler, M L;
CECCHINI, ALESSANDRA LOURENÇO;
MEDEIROS, ALEXANDRA IVO DE;
ALMEIDA, ANA MARISA FUSCO;
et.al
Palavra-chave:
COVID-19;
Genetic diversity;
disease susceptibility;
therapy;
SARS-CoV-2
Áreas do conhecimento:
Ciências da Saúde; Saúde Coletiva; Medicina Preventiva; Águas Contaminadas;
Ciências Biológicas; Genética; Genética Humana e Médica; Imunogenética;
Ciências Biológicas; Farmacologia; Farmacologia Geral
resumo ...
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David Livingstone Alves Figueiredo;
Anelisa Ramão;
Angelica Beate Winter Boldt;
Carla Fredrichsen Moya;
Chung Man Chin;
Daniel de Paula;
Daniel Rech;
Daniela Fiori Gradia;
Danielle Malheiros;
Danielle Venturini;
Eliandro Reis Tavares;
João Paulo Bianchi Ximenez;
Emerson Carraro;
Fábio Rodrigues Ferreira Seiva;
Carolina Panis;
Rafael dos Santos Bezerra;
Adriano Ferrasa;
Alessandra Lourenço Cecchini;
Alexandra Ivo de Medeiros;
Ana Marisa Fusco Almeida
Palavra-chave:
COVID-19;
SARS-CoV-2;
resposta imune;
vacina;
genética epidemiológica
Áreas do conhecimento:
Ciências Biológicas; Bioquímica dos Microorganismos; Enzimologia;
Ciências Biológicas; Genética; Genética Humana e Médica; Imunogenética
resumo ...
Abstract Coronavirus disease 2019 (COVID-19), caused by the Severe Acute Respiratory Syndrome...
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FIGUEIREDO, DAVID LIVINGSTONE ALVES;
RAMÃO, ANELISA;
Boldt, Angelica Beate Winter;
MOYA, CARLA FREDRICHSEN;
CHIN, CHUNG MAN;
PAULA, DANIEL DE;
RECH, DANIEL;
Daniela Fiori Gradia;
MALHEIROS, DANIELLE;
VENTURINI, DANIELLE;
TAVARES, ELIANDRO REIS;
XIMENEZ, JOÃO PAULO BIANCHI;
CARRARO, EMERSON;
SEIVA, FÁBIO RODRIGUES FERREIRA;
PANIS, CAROLINA;
BEZERRA, RAFAEL DOS SANTOS;
FERRASA, ADRIANO;
CECCHINI, ALESSANDRA LOURENÇO;
MEDEIROS, ALEXANDRA IVO DE;
ALMEIDA, ANA MARISA FUSCO
Palavra-chave:
COVID-19;
therapeutic interventions;
global health treat;
virus diversity
Áreas do conhecimento:
Ciências da Saúde; Farmacologia; Transmissão Neuromuscular;
Ciências Biológicas; Genética; Genética Humana e Médica; Imunogenética
resumo ...
Abstract Coronavirus disease 2019 (COVID-19), caused by the Severe Acute Respiratory Syndrome...
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Hanna Karolyna dos Santos;
Patrícia da Silva Dias;
Rafael Ernesto Balen;
Izabel Volkweis Zadinelo;
Robie Allan Bombardelli;
Fábio Meurer
Palavra-chave:
bagre;
desempenho;
peixes nativos;
piscicultura;
zoologia aplicada
Áreas do conhecimento:
CIENCIAS_AGRARIAS; Aqüicultura;
CIENCIAS_AGRARIAS; Zootecnia; Aquicultura; Piscicultura;
Ciências Biológicas; Zoologia; Zoologia Aplicada; Conservação das Espécies Animais
resumo ...
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Paulo de Tarso Cunha Chaves
Palavra-chave:
estuários;
Fishing gear;
Captura incidental;
Pesca-fantasma;
Bycatch
resumo ...
Abstract Comparisons between the implications of commercial and recreational fisheries for...
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Zelinda SCHEMCZSSEN;
Fernando Hitomi Matsubara;
Andrea Senff Ribeiro;
Luiza Helena Gremski;
Silvio Sanches Veiga;
Hanna Camara Justa;
Jenifer Nowatzki;
Baldissera, A.B.;
Nayanne Louise Costacurta Polli;
Elidiana de Bona;
Izadora Volpato Rossi;
Marcel Ivan Ramirez;
João Minozzo
Palavra-chave:
Aranha marrom;
Veneno;
serpina;
Propriedades biológicas;
Propriedades funcionais
Áreas do conhecimento:
Ciências Biológicas; Morfologia; Citologia e Biologia Celular;
Ciências Biológicas; Bioquímica; Biologia Molecular
resumo ...
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DE SOUZA, IRISDORIS RODRIGUES;
LEME, DANIELA MORAIS;
CANAVEZ, ANDREZZA DI PIETRO MICALI;
SCHUCK, DESIREE CIGARAN;
GAGOSIAN, VIVIANA STEPHANIE COSTA;
DE SOUZA, ISISDORIS RODRIGUES;
Taynah Vicari;
DA SILVA TRINDADE, EDVALDO;
Cestari, M.M.;
LORENCINI, MÁRCIO
Palavra-chave:
ensaio cometa;
ZFL;
peixe
Áreas do conhecimento:
Ciências Biológicas; Genética; Genética Animal; Mutagênese Ambiental;
Ciências Biológicas; Genética; genotoxicidade
resumo ...
Fish cell spheroids are promising 3D culture models for vertebrate replacement in ecotoxicology. However, new alternative ecotoxicological methods must be adapted for applications in industry and for regulatory purposes; such methods must be cost-effective, simple to manipulate and provide rapid results. Therefore, we compared the effectiveness of the traditional hanging drop (HD), orbital shaking (OS), and HD combined with OS (HD+OS) methods on the formation of zebrafish cell line spheroids (ZFL and ZEM2S). Time in HD (3–5 days) and different 96-well plates [flat-bottom or ultra-low attachment of round-bottom (ULA-plates)] in OS were evaluated. Easy handling, rapid spheroid formation, uniform-sized spheroids, and circularity were assessed to identify the best spheroid protocol. Traditional HD alone did not result in ZFL spheroid formation, whereas HD (5 days)+OS did. When using the OS, spheroids only formed on the ULA-plate. Both HD+OS and OS were reproducible in size (177.50 ± 2.81 µm and 225.62 ± 19.20 µm, respectively) and circularity (0.83 ± 0.02 and 0.80 ± 0.01, respectively) of ZFL spheroids. Nevertheless, HD+OS required a considerable time to completely form spheroids (10 days) and intensive handling, whereas the OS was fast (5 days of incubation) and simple. OS also yielded reproducible ZEM2S spheroids in 1 day (226.23 ± 0.57 µm diameter and 0.80 ± 0.01 circularity). In conclusion, OS in ULA-plate is an effective and simple spheroid protocol for high-throughput ecotoxicity testing. This study contributes to identify a fast, reproducible, and simple protocol of single piscine spheroid formation in 96-well plates and supports the application of fish 3D model in industry and academia.
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